Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
L403R/G404R/A406S | regulation of activity by phosphorylation | Saccharolobus solfataricus |
L403R/G404R/A406S | engineering of an appropriately located phosphoacceptor serine and cAMP-dependent protein kinase recognition motif to create a phosphorylation site. Km values, Vmax, optimal pH and temperature of mutant are identical to wild-type. Exposure of mutant to ATP and cAMP-dependent protein kinase is accompanied by incorporation of phosphate and by a parallel decrease in catalytic activity. Subsequent treatment with a protein phosphatase releases enzyme-bound 32Pi and restores activity to pretreatment levels | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00011 | - |
CoASH | pH 9.0, 50°C, wild-type | Saccharolobus solfataricus | |
0.00015 | - |
CoASH | pH 9.0, 50°C, mutant L403R/G404R/A406S | Saccharolobus solfataricus | |
0.0009 | - |
NADP+ | pH 9.0, 50°C, wild-type | Saccharolobus solfataricus | |
0.0013 | - |
NADP+ | pH 9.0, 50°C, mutant L403R/G404R/A406S | Saccharolobus solfataricus | |
0.0015 | - |
(R)-mevalonate | pH 9.0, 50°C, wild-type | Saccharolobus solfataricus | |
0.0015 | - |
(R)-mevalonate | wild type enzyme, at pH 9.0 and 50°C | Saccharolobus solfataricus | |
0.0017 | - |
(R)-mevalonate | pH 9.0, 50°C, mutnant L403R/G404R/A406S | Saccharolobus solfataricus | |
0.0017 | - |
(R)-mevalonate | mutant enzyme L403R/G404R/A406S, at pH 9.0 and 50°C | Saccharolobus solfataricus | |
0.0043 | - |
mevaldehyde | pH 5.0, 50°C, wild-type | Saccharolobus solfataricus | |
0.0063 | - |
mevaldehyde | pH 5.0, 50°C, mutant L403R/G404R/A406S | Saccharolobus solfataricus | |
0.045 | - |
3-hydroxy-3-methylglutaryl-CoA | wild type | Saccharolobus solfataricus | |
0.045 | - |
HMG-CoA | pH 5.5, 50°C, wild-type | Saccharolobus solfataricus | |
0.055 | - |
NADPH | wild type | Saccharolobus solfataricus | |
0.055 | - |
NADPH | pH 5.5, 50°C, wild-type | Saccharolobus solfataricus | |
0.076 | - |
3-hydroxy-3-methylglutaryl-CoA | mutant | Saccharolobus solfataricus | |
0.076 | - |
HMG-CoA | pH 5.5, 50°C, mutant L403R/G404R/A406S | Saccharolobus solfataricus | |
0.083 | - |
NADPH | mutant | Saccharolobus solfataricus | |
0.083 | - |
NADPH | pH 5.5, 50°C, mutant L403R/G404R/A406S | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-3-methylglutaryl-CoA + NADPH | Saccharolobus solfataricus | - |
(R)-mevalonate + CoA + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus | - |
enzyme is not regulated by phosphorylation | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.9 | - |
50°C, mutant L403R/G404R/A406S | Saccharolobus solfataricus |
4.9 | - |
50°C, wild-type | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-mevalonate + CoA + 2 NADP+ | - |
Saccharolobus solfataricus | (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | - |
r | |
(R)-mevalonate + CoA + NADP+ | - |
Saccharolobus solfataricus | (S)-3-hydroxy-3-methylglutaryl-CoA + NADPH + H+ | - |
r | |
3-hydroxy-3-methylglutaryl-CoA + NADPH | - |
Saccharolobus solfataricus | (R)-mevalonate + CoA + NADP+ | - |
r | |
3-hydroxy-3-methylglutaryl-CoA + NADPH | first step reaction | Saccharolobus solfataricus | (R)-mevalonate + CoA + NADP+ | - |
r | |
mevaldehyde + NADPH + H+ | - |
Saccharolobus solfataricus | (R)-mevalonate + NADP+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Saccharolobus solfataricus | |
NADPH | - |
Saccharolobus solfataricus |