Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Leishmania major |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
(R,S)-3-hydroxy-3-methylglutaryl-CoA | - |
Leishmania major | |
0.081 | - |
NADPH | - |
Leishmania major |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
alpha4, 4 * 46000, SDS-PAGE | Leishmania major |
184000 | - |
gel filtration | Leishmania major |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-3-methylglutaryl-CoA + NADPH | Leishmania major | - |
(R)-mevalonate + CoA + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Leishmania major |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0335 | - |
- |
Leishmania major |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-3-methylglutaryl-CoA + NADPH | - |
Leishmania major | (R)-mevalonate + CoA + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha4, 4 * 46000, SDS-PAGE | Leishmania major |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Leishmania major |