Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
3-hydroxy-3-methylglutaryl-CoA | - |
Streptomyces sp. | |
0.062 | - |
NADPH | - |
Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
alpha2, 2 * 41000, SDS-PAGE | Streptomyces sp. |
100000 | - |
gel filtration | Streptomyces sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-3-methylglutaryl-CoA + NADPH | Streptomyces sp. | - |
(R)-mevalonate + CoA + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
CL190 | - |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.6 | - |
- |
Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-3-methylglutaryl-CoA + NADPH | - |
Streptomyces sp. | (R)-mevalonate + CoA + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha2, 2 * 41000, SDS-PAGE | Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 60 | - |
Streptomyces sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 60 | - |
Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Streptomyces sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Streptomyces sp. |