Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 10 mM, 15% inhibition | Weissella confusa | |
4-chloromercuribenzoate | weak inhibition | Weissella confusa | |
iodoacetamide | weak inhibition | Weissella confusa | |
iodoacetate | weak inhibition | Weissella confusa | |
KCN | weak inhibition | Weissella confusa | |
additional information | reduced glutathione (20 mM) has no effect. EDTA, 2,2'-bipyridyl in 1 mM and 10 mM final concentrations have no effect on enzymatic activity | Weissella confusa | |
NADH | inhibition above 0.24 mM | Weissella confusa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
2-oxoisocaproate | pH 7.0, 30°C | Weissella confusa | |
0.065 | - |
2-oxoisovalerate | pH 7.0, 30°C | Weissella confusa | |
0.1 | - |
2-oxovalerate | pH 7.0, 30°C | Weissella confusa | |
0.1 | 2 | phenylpyruvate | pH 7.0, 30°C | Weissella confusa | |
0.1 | 2 | 2-oxocaproate | pH 7.0, 30°C | Weissella confusa | |
0.17 | - |
2-oxooctanoate | pH 7.0, 30°C | Weissella confusa | |
0.45 | - |
2-oxobutyrate | pH 7.0, 30°C | Weissella confusa | |
0.6 | - |
2-Hydroxyisovalerate | pH 8.0, 30°C | Weissella confusa | |
0.62 | - |
2-hydroxyisocaproate | pH 8.0, 30°C | Weissella confusa | |
0.64 | - |
3-Phenyllactate | pH 8.0, 30°C | Weissella confusa | |
1.8 | - |
2-hydroxyvalerate | pH 8.0, 30°C | Weissella confusa | |
1.9 | - |
2-Hydroxyoctanoate | pH 8.0, 30°C | Weissella confusa | |
2.2 | - |
2-hydroxycaproate | pH 8.0, 30°C | Weissella confusa | |
4.5 | - |
pyruvate | pH 7.0, 30°C | Weissella confusa | |
100 | - |
L-lactate | pH 8.0, 30°C | Weissella confusa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | at 1 mM no influence is observed with Mg2+, Ca2+, Co2+, Ni2+, and Cd2+ and very weak effects with Mn2+, Cu2+, and Zn2+ | Weissella confusa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
4 * 33000, SDS-PAGE | Weissella confusa |
125000 | - |
gel filtration | Weissella confusa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a 2-oxocarboxylate + NADH + H+ | Weissella confusa | - |
(2S)-2-hydroxycarboxylate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Weissella confusa | P14295 | i.e. Lactobacillus confusus | - |
Purification (Comment) | Organism |
---|---|
- |
Weissella confusa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition | maximum specific and volume activities are found at the end of the exponential growth phase, during further fermentation enzyme activity declines rapidly and is no longer detectable after 15 h | Weissella confusa | - |
Storage Stability | Organism |
---|---|
4°C, pH 6.5, concentrated enzyme solution, 10% loss of activity after 6 months | Weissella confusa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxycaproate + NAD+ | - |
Weissella confusa | 2-oxocaproate + NADH + H+ | - |
r | |
2-hydroxyisocaproate + NAD+ | the initial rate of the reduction of 2-oxoisocaproate is about seven times faster than the reverse reaction, the dehydrogenation of L-2-hydroxyisocaproate, measured in buffers at the respective pH optima | Weissella confusa | 2-oxoisocaproate + NADH + H+ | - |
r | |
2-hydroxyisovalerate + NAD+ | - |
Weissella confusa | 2-oxoisovalerate + NADH + H+ | - |
r | |
2-hydroxyoctanoate + NAD+ | - |
Weissella confusa | 2-oxooctanoate + NADH + H+ | - |
? | |
2-hydroxyvalerate + NAD+ | - |
Weissella confusa | 2-oxovalerate + NADH + H+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Weissella confusa | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxocaproate + NADH + H+ | - |
Weissella confusa | 2-hydroxycaproate + NAD+ | - |
r | |
2-oxoisocaproate + NADH + H+ | 2-oxoisocaproate is the best substrate with the lowest KM-valus of 0.065 mM. Tthe initial rate of the reduction of 2-oxoisocaproate is about seven times faster than the reverse reaction, the dehydrogenation of L-2-hydroxyisocaproate, measured in buffers at the respective pH optima | Weissella confusa | 2-hydroxyisocaproate + NAD+ | - |
r | |
2-oxoisovalerate + NADH + H+ | - |
Weissella confusa | 2-hydroxyisovalerate + NAD+ | - |
r | |
2-oxooctanoate + NADH + H+ | - |
Weissella confusa | 2-hydroxyoctanoate + NAD+ | - |
r | |
2-oxovalerate + NADH + H+ | - |
Weissella confusa | 2-hydroxyvalerate + NAD+ | - |
r | |
3-phenyllactate + NAD+ | - |
Weissella confusa | pyruvate + NADH + H+ | - |
r | |
a 2-oxocarboxylate + NADH + H+ | - |
Weissella confusa | (2S)-2-hydroxycarboxylate + NAD+ | - |
r | |
a 2-oxocarboxylate + NADH + H+ | various 2-oxocarboxylic acids are stereospecifically reduced to the corresponding (S)-2-hydroxycarboxylic acids. In the reverse reaction the NAD+-dependent dehydrogenation of L-2-hydroxycarboxylic acids is observed | Weissella confusa | (2S)-2-hydroxycarboxylate + NAD+ | - |
r | |
L-lactate + NAD+ | - |
Weissella confusa | pyruvate + NADH + H+ | - |
r | |
phenylpyruvate + NADH + H+ | - |
Weissella confusa | 3-phenyllactate + NAD+ | - |
r | |
pyruvate + NADH + H+ | - |
Weissella confusa | L-lactate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 33000, SDS-PAGE | Weissella confusa |
Synonyms | Comment | Organism |
---|---|---|
L-2-hydroxyisocaproate dehydrogenase | - |
Weissella confusa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Weissella confusa |
50 | - |
- |
Weissella confusa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | the maximal temperature is around 50°C. At this temperature the reaction proceeded at about 3fold increase of the rate compared to 30°C | Weissella confusa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
1 h, stable up to | Weissella confusa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
reduction of 2-oxoisocaproate | Weissella confusa |
8 | 8.5 | dehydrogenation of L-2-hydroxyisocaproate | Weissella confusa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | 4°C, 24 h, stable | Weissella confusa |
6.5 | - |
4°C, 6 months, concentrated enzyme solutions, 10% loss of activity | Weissella confusa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the enzyme requires NAD+ as a cofactor, no reaction with NADP+ in concentrations up to 3 mM | Weissella confusa | |
NADH | the enzyme requires NADH as a cofactor, no reaction with NADPH up to 0.24 mM | Weissella confusa |