Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 2 mM, maximum activation | Escherichia coli | |
additional information | the presence of SH compounds is required for activity | Escherichia coli | |
N-acetylglucosamine 1-phosphate | activation at low UDP-N-acetylmannosamine concentrations | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
UDP-N-acetyl-alpha-D-mannosamine | pH 10.0, 37°C, presence of 150 mM KCl | Escherichia coli | |
0.21 | - |
NAD+ | pH 10.0, 37°C | Escherichia coli | |
0.22 | - |
UDP-N-acetyl-alpha-D-mannosamine | pH 10.0, 37°C, presence of 1 mM N-acetylglucosamine 1-phosphate | Escherichia coli | |
0.38 | - |
UDP-N-acetyl-alpha-D-mannosamine | pH 10.0, 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activation at low UDP-N-acetylmannosamine concentrations | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Escherichia coli |
100000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-18°C, 20 mM Tris-HCl, pH 7.9, 3 M KC1, 2 mM 2-mercaptoethanol, and 10% glycerol, storage without loss of activity for 2 weeks, presence of bovine serum albumin stabilizes | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-mannosamine + 2 NAD+ + H2O | enzyme is strictly specific for UDP-N-acetyl-mannosamine and NAD + | Escherichia coli | UDP-N-acetyl-alpha-D-mannosaminuronate + 2 NADH + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | 10 | - |
Escherichia coli |