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Literature summary for 1.1.1.331 extracted from

  • Arneaud, S.L.; Porter, J.R.
    Investigation and expression of the secoisolariciresinol dehydrogenase gene involved in podophyllotoxin biosynthesis (2015), Mol. Biotechnol., 57, 961-973 .
    View publication on PubMed
Search for more literature for 1.1.1.331

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons, cloning and expression in Escherichiaa coli strain JM109. Confirmation that the SD gene sequence originates from Phialocephala podophylli strain PPE7 fungal gDNA and is not a product from amplification of Podophyllum peltatum gDNA plant contamination is achieved through PCR amplification of any contaminating rbcL plant gene sequences in the PPE7 fungal gDNA template samples Phialocephala podophylli
gene SDH_Pp7, DNA and amino acid sequence determination and analysis, sequence comparisons, cloning and expression in Escherichia coli strain JM109, compatible codon optimization for expression in the heterologous host Pichia pastoris Podophyllum peltatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(-)-secoisolariciresinol + 2 NAD+ Podophyllum peltatum
-
 (-)-matairesinol + 2 NADH + 2 H+
-
 ?
(-)-secoisolariciresinol + 2 NAD+ Phialocephala podophylli
-
 (-)-matairesinol + 2 NADH + 2 H+
-
 ?
(-)-secoisolariciresinol + 2 NAD+ Phialocephala podophylli PPE7
-
 (-)-matairesinol + 2 NADH + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Phialocephala podophylli A0A0M5K823 isolated from the rhizomes of Podophyllum peltatum
-
Phialocephala podophylli PPE7 A0A0M5K823 isolated from the rhizomes of Podophyllum peltatum
-
Podophyllum peltatum A0A0M4J2R3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-secoisolariciresinol + 2 NAD+
-
 Podophyllum peltatum (-)-matairesinol + 2 NADH + 2 H+
-
 ?
(-)-secoisolariciresinol + 2 NAD+
-
 Phialocephala podophylli (-)-matairesinol + 2 NADH + 2 H+
-
 ?
(-)-secoisolariciresinol + 2 NAD+
-
 Phialocephala podophylli PPE7 (-)-matairesinol + 2 NADH + 2 H+
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 32000, about, sequence calculation Podophyllum peltatum
More the enzyme forms a homotetramer composed of an alpha/beta single domain structure with a dinucleotide-binding Rossmann fold for the binding of NAD+ and an active site with a highly conserved catalytic triad of amino acids, Ser153, Tyr167 and Lys171 Podophyllum peltatum

Synonyms

Synonyms Comment Organism
PpSD
-
 Podophyllum peltatum
SDH_Pp7
-
 Podophyllum peltatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Podophyllum peltatum
30
-
 assay at Phialocephala podophylli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
 assay at Podophyllum peltatum
8.8
-
 assay at Phialocephala podophylli

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Podophyllum peltatum
NAD+
-
 Phialocephala podophylli

General Information

General Information Comment Organism
metabolism the enzyme is part of the podophyllotoxin, PPT, biosynthetic pathway Podophyllum peltatum
metabolism the enzyme is part of the podophyllotoxin, PPT, biosynthetic pathway Phialocephala podophylli
additional information the enzyme forms a homotetramer composed of an alpha/beta single domain structure with a dinucleotide-binding Rossmann fold for the binding of NAD+ and an active site with a highly conserved catalytic triad of amino acids, Ser153, Tyr167 and Lys171 Podophyllum peltatum
physiological function the enzyme secoisolariciresinol dehydrogenase facilitates the dehydrogenation of secoisolariciresinol to form matairesinol, a mid-pathway intermediate product in podophyllotoxin, PPT, biosynthesis Podophyllum peltatum
physiological function the enzyme secoisolariciresinol dehydrogenase facilitates the dehydrogenation of secoisolariciresinol to form matairesinol, a mid-pathway intermediate product in podophyllotoxin, PPT, biosynthesis Phialocephala podophylli