BRENDA - Enzyme Database show
show all sequences of 1.1.1.33

Some properties and a suggested reclassification of mevaldate reductase

Beedle, A.S.; Rees, H.H.; Goodwin, T.W.; Biochem. J. 139, 205-209 (1974)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Sodium aminobarbitone
1 mM, 48% inhibition, noncompetitive
Sus scrofa
Thiobarbituric acid
1 mM, 22% inhibition, noncompetitive
Sus scrofa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.09
-
NADPH
-
Sus scrofa
0.15
-
4-Cyanobenzaldehyde
-
Sus scrofa
0.26
-
(3RS)-mevaldate
-
Sus scrofa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
gel filtration
Sus scrofa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-3-hydroxy-3-methyl-5-oxovaleric acid + NADPH
Sus scrofa
-
(R)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NADP+
-
Sus scrofa
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sus scrofa
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate precipitation, calcium phosphate gel, gel filtration
Sus scrofa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.573
-
-
Sus scrofa
Storage Stability
Storage Stability
Organism
-20░C, 3 months, 0% loss of activity
Sus scrofa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-3-hydroxy-3-methyl-5-oxovaleric acid + NADPH
-
286556
Sus scrofa
(R)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NADP+
-
286556
Sus scrofa
ir
(S)-3-hydroxy-3-methyl-5-oxovaleric acid + NADH
-
286556
Sus scrofa
(S)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NAD+
-
286556
Sus scrofa
ir
4-cyanobenzaldehyde + NADPH
-
286556
Sus scrofa
4-cyanobenzylalcohol + NADP+
-
286556
Sus scrofa
ir
pyridine-3-carboxaldehyde + NADPH
-
286556
Sus scrofa
pyridine-3-methanol + NADP+
-
286556
Sus scrofa
ir
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
giving one-fifth of the rate obtained with NADPH
Sus scrofa
NADPH
-
Sus scrofa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
giving one-fifth of the rate obtained with NADPH
Sus scrofa
NADPH
-
Sus scrofa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Sodium aminobarbitone
1 mM, 48% inhibition, noncompetitive
Sus scrofa
Thiobarbituric acid
1 mM, 22% inhibition, noncompetitive
Sus scrofa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.09
-
NADPH
-
Sus scrofa
0.15
-
4-Cyanobenzaldehyde
-
Sus scrofa
0.26
-
(3RS)-mevaldate
-
Sus scrofa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
gel filtration
Sus scrofa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-3-hydroxy-3-methyl-5-oxovaleric acid + NADPH
Sus scrofa
-
(R)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NADP+
-
Sus scrofa
ir
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, calcium phosphate gel, gel filtration
Sus scrofa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.573
-
-
Sus scrofa
Storage Stability (protein specific)
Storage Stability
Organism
-20░C, 3 months, 0% loss of activity
Sus scrofa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-3-hydroxy-3-methyl-5-oxovaleric acid + NADPH
-
286556
Sus scrofa
(R)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NADP+
-
286556
Sus scrofa
ir
(S)-3-hydroxy-3-methyl-5-oxovaleric acid + NADH
-
286556
Sus scrofa
(S)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NAD+
-
286556
Sus scrofa
ir
4-cyanobenzaldehyde + NADPH
-
286556
Sus scrofa
4-cyanobenzylalcohol + NADP+
-
286556
Sus scrofa
ir
pyridine-3-carboxaldehyde + NADPH
-
286556
Sus scrofa
pyridine-3-methanol + NADP+
-
286556
Sus scrofa
ir
Other publictions for EC 1.1.1.33
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286556
Beedle
Some properties and a suggeste ...
Sus scrofa
Biochem. J.
139
205-209
1974
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2
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1
1
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1
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4
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2
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2
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2
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3
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1
1
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1
-
1
1
1
4
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