Application | Comment | Organism |
---|---|---|
biotechnology | production of 2-pyrone-4,6-dicarboxylic acid from protocatechuate as a precursor for biopolymers | Sphingomonas paucimobilis |
industry | the authors focus on the metabolic intermediate 2-pyrone-4,6-dicarboxylic acid as a potential raw material for novel, bio-based polymers | Sphingomonas paucimobilis |
Cloned (Comment) | Organism |
---|---|
coexpression with protocatechuate 4,5-dioxygenase in Pseudomonas putida PpY1100 | Sphingomonas paucimobilis |
into the pKT230MC vector for expression in Pseudomonas putida PpY1100 and into pUC119 for DNA amplification in Escherichia coli DH5alpha | Sphingomonas paucimobilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+ + H2O | Sphingomonas paucimobilis | - |
4-carboxy-2-hydroxy-cis,cis-muconate + NADPH + 2 H+ | - |
? | |
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+ + H2O | Sphingomonas paucimobilis SYK-6 | - |
4-carboxy-2-hydroxy-cis,cis-muconate + NADPH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas paucimobilis | - |
- |
- |
Sphingomonas paucimobilis | - |
SYK-6 | - |
Sphingomonas paucimobilis SYK-6 | - |
- |
- |
Sphingomonas paucimobilis SYK-6 | - |
SYK-6 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Sphingomonas paucimobilis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+ + H2O | - |
Sphingomonas paucimobilis | 4-carboxy-2-hydroxy-cis,cis-muconate + NADPH + 2 H+ | - |
? | |
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+ + H2O | - |
Sphingomonas paucimobilis SYK-6 | 4-carboxy-2-hydroxy-cis,cis-muconate + NADPH + 2 H+ | - |
? | |
4-carboxy-2-hydroxymuconate semialdehyde + NADP+ | - |
Sphingomonas paucimobilis | 2-pyrone-4,6-dicarboxylic acid + NADPH + H2O | - |
? | |
4-carboxy-2-hydroxymuconate semialdehyde + NADP+ | - |
Sphingomonas paucimobilis SYK-6 | 2-pyrone-4,6-dicarboxylic acid + NADPH + H2O | - |
? | |
protocatechuate + NADP+ + H2O | - |
Sphingomonas paucimobilis | 2-pyrone-4,6-dicarboxylic acid + NADPH + H+ | - |
? | |
protocatechuate + NADP+ + H2O | - |
Sphingomonas paucimobilis SYK-6 | 2-pyrone-4,6-dicarboxylic acid + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-hydroxy-4-carboxymuconate-6-semialdehyde dehydrogenase | - |
Sphingomonas paucimobilis |
4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase | - |
Sphingomonas paucimobilis |
CHMS dehydrogenase | - |
Sphingomonas paucimobilis |
CHMS hydrolase | - |
Sphingomonas paucimobilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
fermenter condition for production of 2-pyrone-4,6-dicarboxylic acid, PDC | Sphingomonas paucimobilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Sphingomonas paucimobilis |