Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.311 extracted from

  • Hoeffken, H.W.; Duong, M.; Friedrich, T.; Breuer, M.; Hauer, B.; Reinhardt, R.; Rabus, R.; Heider, J.
    Crystal structure and enzyme kinetics of the (S)-specific 1-phenylethanol dehydrogenase of the denitrifying bacterium strain EbN1 (2006), Biochemistry, 45, 82-93.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Azoarcus sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop method in a buffer containing 12-18% PEG 8000, 0.05 M potassium phosphate, pH 7.5 and 3% 2-methyl-2,4-pentanediol. The structure of the apo-form of the enzyme is solved with a resolution at 2.1 A. The enzyme soaked with NAD+ and the inhibitor (R)-1-phenylethanol forms a different crystal form containing two subunits in the asymmetric unit Azoarcus sp.

Inhibitors

Inhibitors Comment Organism Structure
(R)-1-phenylethanol 0.05 mM, 50% inhibition of acetophenone reduction activity Azoarcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
4 * 27000 Azoarcus sp.
93000
-
gel filtration Azoarcus sp.

Organism

Organism UniProt Comment Textmining
Azoarcus sp. Q5P5I4
-
-
Azoarcus sp. EbN1 Q5P5I4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Azoarcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-1-phenylethanol + NAD+
-
Azoarcus sp. acetophenone + NADH + H+
-
r
(S)-1-phenylethanol + NAD+
-
Azoarcus sp. EbN1 acetophenone + NADH + H+
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 27000 Azoarcus sp.

Synonyms

Synonyms Comment Organism
PED
-
Azoarcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Azoarcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Azoarcus sp.