General Stability | Organism |
---|---|
at 4°C, SlcC remains stable for several weeks and can be frozen and thawed without loss of activity | Chromohalobacter salexigens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | SlcC is not inhibited by pyruvate or oxaloacetate (5 mM), (S)-3-phosphoglycerate, (R)-malate, (R)-lactate, (S)-malate or (S)-lactate | Chromohalobacter salexigens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
NADH | apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C | Chromohalobacter salexigens | |
0.14 | - |
3-sulfopyruvate | apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C | Chromohalobacter salexigens | |
2 | - |
NAD+ | apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C | Chromohalobacter salexigens | |
7 | - |
(2S)-3-sulfolactate | apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C | Chromohalobacter salexigens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
2 * 34000, SDS-PAGE | Chromohalobacter salexigens |
61000 | - |
native enzyme, gel filtration | Chromohalobacter salexigens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chromohalobacter salexigens | - |
- |
- |
Chromohalobacter salexigens DSM 3043 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Mono Q column chromatography, phenyl Superose gel filtration, Superose 12 gel filtration | Chromohalobacter salexigens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:acetate-grown cell | - |
Chromohalobacter salexigens | - |
culture condition:sulfolactate-grown cell | - |
Chromohalobacter salexigens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.2 | - |
crude extracts of acetate-grown cells, using 3-sulfopyruvate as substrate, at pH 9.0 and 25°C | Chromohalobacter salexigens |
7.4 | - |
crude extracts of sulfolactate-grown cells, using 3-sulfopyruvate as substrate, at pH 9.0 and 25°C | Chromohalobacter salexigens |
675 | - |
enzyme from sulfolactate-grown cells after 91fold purification, using 3-sulfopyruvate as substrate, at pH 9.0 and 25°C | Chromohalobacter salexigens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-3-sulfolactate + NAD+ | the specific activity is only about 5% of that in the reverse direction | Chromohalobacter salexigens | 3-sulfopyruvate + NADH + H+ | - |
r | |
(2S)-3-sulfolactate + NAD+ | the specific activity is only about 5% of that in the reverse direction | Chromohalobacter salexigens DSM 3043 | 3-sulfopyruvate + NADH + H+ | - |
r | |
3-sulfopyruvate + NADH + H+ | - |
Chromohalobacter salexigens | (2S)-3-sulfolactate + NAD+ | - |
r | |
3-sulfopyruvate + NADH + H+ | - |
Chromohalobacter salexigens DSM 3043 | (2S)-3-sulfolactate + NAD+ | - |
r | |
additional information | neither pyruvate, oxaloacetate, (S)-3-phosphoglycerate, (R)-malate, (R)-lactate, (S)-malate or (S)-lactate is a substrate | Chromohalobacter salexigens | ? | - |
? | |
additional information | neither pyruvate, oxaloacetate, (S)-3-phosphoglycerate, (R)-malate, (R)-lactate, (S)-malate or (S)-lactate is a substrate | Chromohalobacter salexigens DSM 3043 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 34000, SDS-PAGE | Chromohalobacter salexigens |
Synonyms | Comment | Organism |
---|---|---|
(S)-sulfolactate oxidoreductase | - |
Chromohalobacter salexigens |
SlcC | - |
Chromohalobacter salexigens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
at 4°C, SlcC remains stable for several weeks and can be frozen and thawed without loss of activity | Chromohalobacter salexigens |