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Literature summary for 1.1.1.310 extracted from

  • Denger, K.; Cook, A.M.
    Racemase activity effected by two dehydrogenases in sulfolactate degradation by Chromohalobacter salexigens: purification of (S)-sulfolactate dehydrogenase (2010), Microbiology, 156, 967-974.
    View publication on PubMed
Search for more literature for 1.1.1.310

General Stability

General Stability Organism
at 4°C, SlcC remains stable for several weeks and can be frozen and thawed without loss of activity Chromohalobacter salexigens

Inhibitors

Inhibitors Comment Organism Structure
additional information SlcC is not inhibited by pyruvate or oxaloacetate (5 mM), (S)-3-phosphoglycerate, (R)-malate, (R)-lactate, (S)-malate or (S)-lactate Chromohalobacter salexigens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 NADH apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C Chromohalobacter salexigens
0.14
-
 3-sulfopyruvate apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C Chromohalobacter salexigens
2
-
 NAD+ apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C Chromohalobacter salexigens
7
-
 (2S)-3-sulfolactate apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C Chromohalobacter salexigens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 2 * 34000, SDS-PAGE Chromohalobacter salexigens
61000
-
 native enzyme, gel filtration Chromohalobacter salexigens

Organism

Organism UniProt Comment Textmining
Chromohalobacter salexigens
-
-
-
Chromohalobacter salexigens DSM 3043
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Mono Q column chromatography, phenyl Superose gel filtration, Superose 12 gel filtration Chromohalobacter salexigens

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:acetate-grown cell
-
 Chromohalobacter salexigens
-
culture condition:sulfolactate-grown cell
-
 Chromohalobacter salexigens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2
-
 crude extracts of acetate-grown cells, using 3-sulfopyruvate as substrate, at pH 9.0 and 25°C Chromohalobacter salexigens
7.4
-
 crude extracts of sulfolactate-grown cells, using 3-sulfopyruvate as substrate, at pH 9.0 and 25°C Chromohalobacter salexigens
675
-
 enzyme from sulfolactate-grown cells after 91fold purification, using 3-sulfopyruvate as substrate, at pH 9.0 and 25°C Chromohalobacter salexigens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-3-sulfolactate + NAD+ the specific activity is only about 5% of that in the reverse direction Chromohalobacter salexigens 3-sulfopyruvate + NADH + H+
-
 r
(2S)-3-sulfolactate + NAD+ the specific activity is only about 5% of that in the reverse direction Chromohalobacter salexigens DSM 3043 3-sulfopyruvate + NADH + H+
-
 r
3-sulfopyruvate + NADH + H+
-
 Chromohalobacter salexigens (2S)-3-sulfolactate + NAD+
-
 r
3-sulfopyruvate + NADH + H+
-
 Chromohalobacter salexigens DSM 3043 (2S)-3-sulfolactate + NAD+
-
 r
additional information neither pyruvate, oxaloacetate, (S)-3-phosphoglycerate, (R)-malate, (R)-lactate, (S)-malate or (S)-lactate is a substrate Chromohalobacter salexigens ?
-
 ?
additional information neither pyruvate, oxaloacetate, (S)-3-phosphoglycerate, (R)-malate, (R)-lactate, (S)-malate or (S)-lactate is a substrate Chromohalobacter salexigens DSM 3043 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 34000, SDS-PAGE Chromohalobacter salexigens

Synonyms

Synonyms Comment Organism
(S)-sulfolactate oxidoreductase
-
 Chromohalobacter salexigens
SlcC
-
 Chromohalobacter salexigens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 at 4°C, SlcC remains stable for several weeks and can be frozen and thawed without loss of activity Chromohalobacter salexigens