BRENDA - Enzyme Database show
show all sequences of 1.1.1.31

3-Hydroxyisobutyrate dehydrogenase-I from Pseudomonas denitrificans ATCC 13867 degrades 3-hydroxypropionic acid

Lee, P.; Raj, S.; Zhou, S.; Ashok, S.; Edwardraja, S.; Park, S.; Biotechnol. Bioprocess Eng. 19, 1-7 (2014)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
about 1575.34% activity at 1 mM
Pseudomonas denitrificans
dithiothreitol
about 182.11% activity at 1 mM
Pseudomonas denitrificans
EDTA
about 156.21% activity at 1 mM
Pseudomonas denitrificans
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Pseudomonas denitrificans
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
complete inhibition at 1 mM
Pseudomonas denitrificans
Co2+
about 68.1% residual activity at 1 mM
Pseudomonas denitrificans
Cu2+
about 15.67% residual activity at 1 mM
Pseudomonas denitrificans
Fe2+
complete inhibition at 1 mM
Pseudomonas denitrificans
Hg2+
complete inhibition at 1 mM
Pseudomonas denitrificans
K+
about 92.11% residual activity at 1 mM
Pseudomonas denitrificans
Li+
about 92.32% residual activity at 1 mM
Pseudomonas denitrificans
Mg2+
about 87.34% residual activity at 1 mM
Pseudomonas denitrificans
NH4+
about 90.17% residual activity at 1 mM
Pseudomonas denitrificans
Ni2+
about 63.67% residual activity at 1 mM
Pseudomonas denitrificans
Zn2+
about 16.13% residual activity at 1 mM
Pseudomonas denitrificans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
(S)-3-hydroxyisobutyrate
at 37°C and pH 9.0
Pseudomonas denitrificans
2.09
-
3-hydroxypropionic acid
at 37°C and pH 9.0
Pseudomonas denitrificans
10.37
-
methyl DL-serine
at 37°C and pH 9.0
Pseudomonas denitrificans
10.48
-
methyl (2S)-3-hydroxy-2-methylpropanoate
at 37°C and pH 9.0
Pseudomonas denitrificans
31.84
-
L-serine
at 37°C and pH 9.0
Pseudomonas denitrificans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
about 127.2% activity at 1 mM
Pseudomonas denitrificans
additional information
not influenced by Na+
Pseudomonas denitrificans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas denitrificans
-
-
-
Pseudomonas denitrificans ATCC 13867
-
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography and Superdex 200 gel filtration
Pseudomonas denitrificans
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.62
-
crude extract, at 37°C and pH 9.0
Pseudomonas denitrificans
58.67
-
after 36.22fold purification, at 37°C and pH 9.0
Pseudomonas denitrificans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-3-hydroxyisobutyrate + NAD+
the enzyme is very specific to (S)-3-hydroxyisobutyrate
737911
Pseudomonas denitrificans
2-methyl-3-oxopropanoate + NADH + H+
-
-
-
?
(S)-3-hydroxyisobutyrate + NAD+
the enzyme is very specific to (S)-3-hydroxyisobutyrate
737911
Pseudomonas denitrificans ATCC 13867
2-methyl-3-oxopropanoate + NADH + H+
-
-
-
?
3-hydroxypropionic acid + NAD+
-
737911
Pseudomonas denitrificans
malonate semialdehyde + NADH + H+
-
-
-
r
3-hydroxypropionic acid + NAD+
-
737911
Pseudomonas denitrificans ATCC 13867
malonate semialdehyde + NADH + H+
-
-
-
r
L-serine + NAD+
-
737911
Pseudomonas denitrificans
3-oxo-L-alanine + NADH + H+
-
-
-
r
L-serine + NAD+
-
737911
Pseudomonas denitrificans ATCC 13867
3-oxo-L-alanine + NADH + H+
-
-
-
r
methyl (2S)-3-hydroxy-2-methylpropanoate + NAD+
-
737911
Pseudomonas denitrificans
methyl (2S)-2-methyl-3-oxopropanoate + NADH + H+
-
-
-
r
methyl (2S)-3-hydroxy-2-methylpropanoate + NAD+
-
737911
Pseudomonas denitrificans ATCC 13867
methyl (2S)-2-methyl-3-oxopropanoate + NADH + H+
-
-
-
r
methyl DL-serine + NAD+
-
737911
Pseudomonas denitrificans
methyl 3-oxo-DL-alanine + NADH + H+
-
-
-
r
additional information
no activity with (R)-3-hydroxyisobutyrate
737911
Pseudomonas denitrificans
?
-
-
-
-
additional information
no activity with (R)-3-hydroxyisobutyrate
737911
Pseudomonas denitrificans ATCC 13867
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 25000, SDS-PAGE
Pseudomonas denitrificans
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Pseudomonas denitrificans
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
45
more than 50% activity between 30 and 40°C. Approximately 80% of the activity is lost at 45°C
Pseudomonas denitrificans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
7.94
-
methyl DL-serine
at 37°C and pH 9.0
Pseudomonas denitrificans
32.17
-
3-hydroxypropionic acid
at 37°C and pH 9.0
Pseudomonas denitrificans
41.35
-
methyl (2S)-3-hydroxy-2-methylpropanoate
at 37°C and pH 9.0
Pseudomonas denitrificans
47.16
-
L-serine
at 37°C and pH 9.0
Pseudomonas denitrificans
70.81
-
(S)-3-hydroxyisobutyrate
at 37°C and pH 9.0
Pseudomonas denitrificans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Pseudomonas denitrificans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
8
10
approximately 40% of the activity is retained at pH 11.0. At pH 7.0, the activity is reduced to 30% of the activity observed at pH 9.0
Pseudomonas denitrificans
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
the enzyme prefers NAD+ over NADP+ as a cofactor
Pseudomonas denitrificans
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
about 1575.34% activity at 1 mM
Pseudomonas denitrificans
dithiothreitol
about 182.11% activity at 1 mM
Pseudomonas denitrificans
EDTA
about 156.21% activity at 1 mM
Pseudomonas denitrificans
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Pseudomonas denitrificans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
the enzyme prefers NAD+ over NADP+ as a cofactor
Pseudomonas denitrificans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
complete inhibition at 1 mM
Pseudomonas denitrificans
Co2+
about 68.1% residual activity at 1 mM
Pseudomonas denitrificans
Cu2+
about 15.67% residual activity at 1 mM
Pseudomonas denitrificans
Fe2+
complete inhibition at 1 mM
Pseudomonas denitrificans
Hg2+
complete inhibition at 1 mM
Pseudomonas denitrificans
K+
about 92.11% residual activity at 1 mM
Pseudomonas denitrificans
Li+
about 92.32% residual activity at 1 mM
Pseudomonas denitrificans
Mg2+
about 87.34% residual activity at 1 mM
Pseudomonas denitrificans
NH4+
about 90.17% residual activity at 1 mM
Pseudomonas denitrificans
Ni2+
about 63.67% residual activity at 1 mM
Pseudomonas denitrificans
Zn2+
about 16.13% residual activity at 1 mM
Pseudomonas denitrificans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
(S)-3-hydroxyisobutyrate
at 37°C and pH 9.0
Pseudomonas denitrificans
2.09
-
3-hydroxypropionic acid
at 37°C and pH 9.0
Pseudomonas denitrificans
10.37
-
methyl DL-serine
at 37°C and pH 9.0
Pseudomonas denitrificans
10.48
-
methyl (2S)-3-hydroxy-2-methylpropanoate
at 37°C and pH 9.0
Pseudomonas denitrificans
31.84
-
L-serine
at 37°C and pH 9.0
Pseudomonas denitrificans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
about 127.2% activity at 1 mM
Pseudomonas denitrificans
additional information
not influenced by Na+
Pseudomonas denitrificans
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography and Superdex 200 gel filtration
Pseudomonas denitrificans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.62
-
crude extract, at 37°C and pH 9.0
Pseudomonas denitrificans
58.67
-
after 36.22fold purification, at 37°C and pH 9.0
Pseudomonas denitrificans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-3-hydroxyisobutyrate + NAD+
the enzyme is very specific to (S)-3-hydroxyisobutyrate
737911
Pseudomonas denitrificans
2-methyl-3-oxopropanoate + NADH + H+
-
-
-
?
(S)-3-hydroxyisobutyrate + NAD+
the enzyme is very specific to (S)-3-hydroxyisobutyrate
737911
Pseudomonas denitrificans ATCC 13867
2-methyl-3-oxopropanoate + NADH + H+
-
-
-
?
3-hydroxypropionic acid + NAD+
-
737911
Pseudomonas denitrificans
malonate semialdehyde + NADH + H+
-
-
-
r
3-hydroxypropionic acid + NAD+
-
737911
Pseudomonas denitrificans ATCC 13867
malonate semialdehyde + NADH + H+
-
-
-
r
L-serine + NAD+
-
737911
Pseudomonas denitrificans
3-oxo-L-alanine + NADH + H+
-
-
-
r
L-serine + NAD+
-
737911
Pseudomonas denitrificans ATCC 13867
3-oxo-L-alanine + NADH + H+
-
-
-
r
methyl (2S)-3-hydroxy-2-methylpropanoate + NAD+
-
737911
Pseudomonas denitrificans
methyl (2S)-2-methyl-3-oxopropanoate + NADH + H+
-
-
-
r
methyl (2S)-3-hydroxy-2-methylpropanoate + NAD+
-
737911
Pseudomonas denitrificans ATCC 13867
methyl (2S)-2-methyl-3-oxopropanoate + NADH + H+
-
-
-
r
methyl DL-serine + NAD+
-
737911
Pseudomonas denitrificans
methyl 3-oxo-DL-alanine + NADH + H+
-
-
-
r
additional information
no activity with (R)-3-hydroxyisobutyrate
737911
Pseudomonas denitrificans
?
-
-
-
-
additional information
no activity with (R)-3-hydroxyisobutyrate
737911
Pseudomonas denitrificans ATCC 13867
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 25000, SDS-PAGE
Pseudomonas denitrificans
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Pseudomonas denitrificans
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
45
more than 50% activity between 30 and 40°C. Approximately 80% of the activity is lost at 45°C
Pseudomonas denitrificans
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
7.94
-
methyl DL-serine
at 37°C and pH 9.0
Pseudomonas denitrificans
32.17
-
3-hydroxypropionic acid
at 37°C and pH 9.0
Pseudomonas denitrificans
41.35
-
methyl (2S)-3-hydroxy-2-methylpropanoate
at 37°C and pH 9.0
Pseudomonas denitrificans
47.16
-
L-serine
at 37°C and pH 9.0
Pseudomonas denitrificans
70.81
-
(S)-3-hydroxyisobutyrate
at 37°C and pH 9.0
Pseudomonas denitrificans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Pseudomonas denitrificans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
8
10
approximately 40% of the activity is retained at pH 11.0. At pH 7.0, the activity is reduced to 30% of the activity observed at pH 9.0
Pseudomonas denitrificans
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.75
-
methyl DL-serine
at 37°C and pH 9.0
Pseudomonas denitrificans
1.46
-
L-serine
at 37°C and pH 9.0
Pseudomonas denitrificans
4.04
-
methyl (2S)-3-hydroxy-2-methylpropanoate
at 37°C and pH 9.0
Pseudomonas denitrificans
15.4
-
3-hydroxypropionic acid
at 37°C and pH 9.0
Pseudomonas denitrificans
604.1
-
(S)-3-hydroxyisobutyrate
at 37°C and pH 9.0
Pseudomonas denitrificans
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.75
-
methyl DL-serine
at 37°C and pH 9.0
Pseudomonas denitrificans
1.46
-
L-serine
at 37°C and pH 9.0
Pseudomonas denitrificans
4.04
-
methyl (2S)-3-hydroxy-2-methylpropanoate
at 37°C and pH 9.0
Pseudomonas denitrificans
15.4
-
3-hydroxypropionic acid
at 37°C and pH 9.0
Pseudomonas denitrificans
604.1
-
(S)-3-hydroxyisobutyrate
at 37°C and pH 9.0
Pseudomonas denitrificans
Other publictions for EC 1.1.1.31
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737620
Park
Structural and biochemical cha ...
Bacillus cereus
Biochem. Biophys. Res. Commun.
474
522-527
2016
-
-
1
-
-
-
-
2
-
-
1
-
-
2
-
-
1
-
-
-
-
-
4
1
-
-
-
2
-
1
-
1
-
-
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-
-
1
1
-
-
-
-
-
-
2
-
-
1
-
-
-
-
1
-
-
-
-
4
1
-
-
-
2
-
1
-
-
-
-
-
-
2
2
737911
Lee
-
3-Hydroxyisobutyrate dehydroge ...
Pseudomonas denitrificans, Pseudomonas denitrificans ATCC 13867
Biotechnol. Bioprocess Eng.
19
1-7
2014
3
-
1
-
-
-
11
5
-
2
-
-
-
3
-
-
1
-
-
-
2
-
11
1
1
1
-
5
1
1
-
1
-
-
-
3
-
1
1
-
-
-
-
11
-
5
-
2
-
-
-
-
-
1
-
-
2
-
11
1
1
1
-
5
1
1
-
-
-
-
-
-
5
5
722472
Tasi
Characterization of 3-hydroxyi ...
Homo sapiens
J. Assist. Reprod. Genet.
30
505-512
2013
-
-
-
-
-
-
-
-
2
-
-
1
-
3
-
-
-
-
-
11
-
-
1
-
1
-
-
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1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
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1
-
-
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-
-
11
-
-
1
-
1
-
-
-
1
-
-
-
2
3
3
2
-
-
739465
Zhou
Cloning, expression and charac ...
Pseudomonas denitrificans, Pseudomonas denitrificans ATCC 13867
PLoS ONE
8
e62666
2013
3
-
1
-
-
-
8
6
-
2
-
-
-
6
-
-
1
-
-
-
-
-
10
1
1
1
-
6
1
1
-
1
-
-
-
3
-
1
1
-
-
-
-
8
-
6
-
2
-
-
-
-
-
1
-
-
-
-
10
1
1
1
-
6
1
1
-
-
-
-
-
-
6
6
695710
Yao
The catalytic property of 3-hy ...
Bacillus cereus, Bacillus cereus ATCC 14579, Bacillus cereus ATCC14579
Appl. Biochem. Biotechnol.
160
694-703
2009
1
1
1
-
-
-
3
3
-
1
1
9
-
10
-
-
1
-
-
-
3
1
18
1
1
1
1
1
2
1
-
6
-
-
-
1
1
1
6
-
-
-
-
3
-
3
-
1
1
9
-
-
-
1
-
-
3
1
18
1
1
1
1
1
2
1
-
-
-
1
1
-
1
1
688515
Murin
Expression of 3-hydroxyisobuty ...
Rattus norvegicus
J. Neurochem.
105
1176-1186
2008
-
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2
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1
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2
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5
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1
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2
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1
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5
-
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1
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-
-
-
-
-
-
-
-
-
670284
Loupatty
Clinical, biochemical, and mol ...
Homo sapiens
Mol. Genet. Metab.
87
243-248
2006
-
-
1
-
-
-
-
2
-
-
-
-
-
3
-
-
1
-
-
1
3
-
1
-
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-
-
-
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-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
1
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655051
Chowdhury
Cloning and overexpression of ...
Pseudomonas putida, Pseudomonas putida E23
Biosci. Biotechnol. Biochem.
67
438-441
2003
-
-
1
-
-
-
-
-
-
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2
-
-
4
-
-
1
-
-
-
-
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2
1
-
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-
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-
1
-
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1
1
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-
-
2
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286550
Hawes
Mammalien 3-hydroxyisobutyrate ...
Homo sapiens, Oryctolagus cuniculus, Rattus norvegicus
Methods Enzymol.
324
218-228
2000
-
-
2
-
-
-
-
2
-
-
-
-
-
3
-
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3
-
-
1
-
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2
-
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-
-
-
-
-
-
2
-
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2
-
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-
-
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-
3
-
1
-
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2
-
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286551
Chowdhury
3-Hydroxyisobutyrate dehydroge ...
Candida rugosa, Oryctolagus cuniculus, Pseudomonas putida, Pseudomonas putida E23
Biosci. Biotechnol. Biochem.
60
2043-2047
1996
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7
10
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4
3
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7
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1
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1
2
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10
3
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3
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3
3
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3
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7
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10
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4
3
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1
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1
2
-
10
3
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3
-
3
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286552
Hawes
Structural and mechanistic asp ...
Rattus norvegicus
Adv. Exp. Med. Biol.
414
395-402
1996
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1
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8
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1
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1
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1
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1
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8
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1
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1
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286544
Rougraff
Purification and characterizat ...
Oryctolagus cuniculus
J. Biol. Chem.
263
327-331
1988
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2
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2
1
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2
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1
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3
1
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1
1
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-
2
1
1
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1
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1
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-
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-
2
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-
2
1
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1
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3
1
-
1
1
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2
1
1
-
-
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286523
Worral
3-Hydroxyisobutyrate dehydroge ...
Rhodobacter sphaeroides
Biochem. J.
241
297-300
1987
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2
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1
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4
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1
1
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1
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1
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1
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2
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1
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1
1
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1
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286546
Hasegawa
-
Distribution in organisms and ...
Bos taurus, Candida parapsilosis, Candida rugosa, Corynebacterium hydrocarbons, Endomyces reessii, Micrococcus flavus, Micrococcus luteus, Nocardia lyena, Oryctolagus cuniculus, Pseudomonas aeruginosa, Pseudomonas dacunhae, Rhodotorula mucilaginosa, Sus scrofa, Trichosporon aculeatum, Tsukamurella paurometabola
Agric. Biol. Chem.
45
2899-2901
1981
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16
-
15
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1
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4
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16
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3
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3
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16
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1
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4
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16
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286547
Hasegawa
-
Purification, crystallization ...
Candida rugosa
Agric. Biol. Chem.
45
2805-2814
1981
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1
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3
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2
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1
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1
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1
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1
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1
1
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3
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2
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1
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1
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286548
De Paul Marshall
Regulation of valine catabolis ...
Pseudomonas putida
J. Bacteriol.
110
1073-1081
1972
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1
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1
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1
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286549
Robinson
The purification and propertie ...
Sus scrofa
J. Biol. Chem.
225
511-521
1956
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-
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3
2
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1
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1
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3
1
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1
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1
1
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1
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1
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3
-
2
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1
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3
1
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1
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1
1
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