BRENDA - Enzyme Database show
show all sequences of 1.1.1.31

The catalytic property of 3-hydroxyisobutyrate dehydrogenase from Bacillus cereus on 3-hydroxypropionate

Yao, T.; Xu, L.; Ying, H.; Huang, H.; Yan, M.; Appl. Biochem. Biotechnol. 160, 694-703 (2009)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
ethylene glycol tetraacetic acid or EDTA (all at 0.005 and 0.2 mM) do not affect 3-HIBADH activity
Bacillus cereus
Application
Application
Commentary
Organism
additional information
3-HIBADH may play a role in biosynthesis of 3-hydroxypropionate as a biological source
Bacillus cereus
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21 (DE3); MmsB gene, overexpression in Escherichia coli strain BL21, subcloning in strain DH5alpha; pEB81 recombinant vector introduced into Escherichia coli BL21 to overexpress 3-HIBADH protein
Bacillus cereus
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
is not inhibited by 1-propanol, lactate potassium, malate potassium, malonate potassium, acetaldehyde, ethanol, and glycerin
Bacillus cereus
Zn2+
inhibits at 0.2 mM
Bacillus cereus
ZnCl2
0.2 mM, 60% inhibition; inhibits approximately 60% of 3-HIBADH activity at 0.2 mM but shows no effect at 0.005 mM
Bacillus cereus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.25
-
NADP+
; pH 8.5, 37C
Bacillus cereus
2.4
-
NAD+
; pH 8.5, 37C
Bacillus cereus
16.8
-
3-hydroxypropionate
; pH 8.5, 37C, KM-value for 3-hydroxyisobutyrate is approximately 20fold lower
Bacillus cereus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
has no metal ion requirement, MnSO4, CuSO4, CaCl2, MgSO4, and FeSO4 have no effect; no metal ion requirement
Bacillus cereus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
gel filtration
Bacillus cereus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-hydroxy-2-methylpropanoate + NAD+
Bacillus cereus
-
2-methyl-3-oxopropanoate + NADH + H+
-
-
r
3-hydroxy-2-methylpropanoate + NAD+
Bacillus cereus ATCC 14579
-
2-methyl-3-oxopropanoate + NADH + H+
-
-
r
3-hydroxy-2-methylpropanoate + NAD+
Bacillus cereus ATCC14579
-
2-methyl-3-oxopropanoate + NADH + H+
-
-
r
3-hydroxy-2-methylpropanoate + NADP+
Bacillus cereus
-
2-methyl-3-oxopropanoate + NADPH + H+
-
-
r
3-hydroxy-2-methylpropanoate + NADP+
Bacillus cereus ATCC 14579
-
2-methyl-3-oxopropanoate + NADPH + H+
-
-
r
3-hydroxy-2-methylpropanoate + NADP+
Bacillus cereus ATCC14579
-
2-methyl-3-oxopropanoate + NADPH + H+
-
-
r
additional information
Bacillus cereus
MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59
?
-
-
-
additional information
Bacillus cereus ATCC 14579
MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59
?
-
-
-
additional information
Bacillus cereus ATCC14579
MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus cereus
-
; ATCC 14579; MmsB gene
-
Bacillus cereus ATCC 14579
-
-
-
Bacillus cereus ATCC14579
-
MmsB gene
-
Purification (Commentary)
Commentary
Organism
96fold purified at 4C by ammonium sulfate fractionation and gel filtration; ammonium sulfate precipitation, hydrophobic interaction chromatography (Phenyl-Sepharose), anion exchange chromatography; native enzyme 100fold by ammonium sulfate fractionation, and hydrophobic interaction and anion exchange chromatography
Bacillus cereus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.091
-
homogenate
Bacillus cereus
8.7
-
purified enzyme
Bacillus cereus
8.77
-
pH 8.5, 37C; purified enzyme, pH 8.5, 37C
Bacillus cereus
Storage Stability
Storage Stability
Organism
-20C, 50 mM Tris-HCl buffer, pH 8.5, 2 mM EDTA, 2 mM DTT, 50% glycerol
Bacillus cereus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-hydroxy-2-methylpropanoate + NAD+
-
695710
Bacillus cereus
2-methyl-3-oxopropanoate + NADH + H+
-
-
-
r
3-hydroxy-2-methylpropanoate + NAD+
-
695710
Bacillus cereus ATCC 14579
2-methyl-3-oxopropanoate + NADH + H+
-
-
-
r
3-hydroxy-2-methylpropanoate + NAD+
-
695710
Bacillus cereus ATCC14579
2-methyl-3-oxopropanoate + NADH + H+
-
-
-
r
3-hydroxy-2-methylpropanoate + NADP+
-
695710
Bacillus cereus
2-methyl-3-oxopropanoate + NADPH + H+
-
-
-
r
3-hydroxy-2-methylpropanoate + NADP+
-
695710
Bacillus cereus ATCC 14579
2-methyl-3-oxopropanoate + NADPH + H+
-
-
-
r
3-hydroxy-2-methylpropanoate + NADP+
-
695710
Bacillus cereus ATCC14579
2-methyl-3-oxopropanoate + NADPH + H+
-
-
-
r
3-hydroxyisobutyrate + NAD+
-
695710
Bacillus cereus
methylmalonate semialdehyde + NADH + H+
-
-
-
r
3-hydroxypropanoate + NAD(P)+
is more active with NADP+ than NAD+
695710
Bacillus cereus
?
-
-
-
?
3-hydroxypropanoate + NAD(P)+
is more active with NADP+ than NAD+
695710
Bacillus cereus ATCC 14579
?
-
-
-
?
3-hydroxypropanoate + NAD(P)+
is more active with NADP+ than NAD+
695710
Bacillus cereus ATCC14579
?
-
-
-
?
3-hydroxypropanoate + NAD+
-
695710
Bacillus cereus
malonate semialdehyde + NADH + H+
-
-
-
r
3-hydroxypropanoate + NADP+
-
695710
Bacillus cereus
malonate semialdehyde + NADPH + H+
-
-
-
r
additional information
MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59
695710
Bacillus cereus
?
-
-
-
-
additional information
wide substrate specificity of the 3-HIBADH
695710
Bacillus cereus
?
-
-
-
-
additional information
MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59
695710
Bacillus cereus ATCC 14579
?
-
-
-
-
additional information
wide substrate specificity of the 3-HIBADH
695710
Bacillus cereus ATCC 14579
?
-
-
-
-
additional information
MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59
695710
Bacillus cereus ATCC14579
?
-
-
-
-
additional information
wide substrate specificity of the 3-HIBADH
695710
Bacillus cereus ATCC14579
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 32000, SDS-PAGE
Bacillus cereus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
; assay at
Bacillus cereus
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
35
45
shows more than 90% of its optimized activity
Bacillus cereus
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
45
-
enzyme activity is not decreased during preincubation at 45C within 30 min, but preincubation at 55C in 3 min can significantly denaturate and deactivate the enzyme; stable at 45C for 30 min, about 40% activity after 3 min at 55C
Bacillus cereus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.21
-
3-hydroxypropionate
; pH 8.5, 37C
Bacillus cereus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
assay at
Bacillus cereus
8.8
9
; oxidation of 3-hydroxypropionate by 3-HIBADH
Bacillus cereus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
10
; oxidation of 3-hydroxypropionate by 3-HIBADH
Bacillus cereus
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
3-HIBADH is more active with NADP+ than NAD+
Bacillus cereus
NAD(P)+
is more active with NADP+ than NAD+
Bacillus cereus
NAD+
-
Bacillus cereus
NADH
-
Bacillus cereus
NADP+
-
Bacillus cereus
NADPH
-
Bacillus cereus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
ethylene glycol tetraacetic acid or EDTA (all at 0.005 and 0.2 mM) do not affect 3-HIBADH activity
Bacillus cereus
Application (protein specific)
Application
Commentary
Organism
additional information
3-HIBADH may play a role in biosynthesis of 3-hydroxypropionate as a biological source
Bacillus cereus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21 (DE3); MmsB gene, overexpression in Escherichia coli strain BL21, subcloning in strain DH5alpha; pEB81 recombinant vector introduced into Escherichia coli BL21 to overexpress 3-HIBADH protein
Bacillus cereus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
3-HIBADH is more active with NADP+ than NAD+
Bacillus cereus
NAD(P)+
is more active with NADP+ than NAD+
Bacillus cereus
NAD+
-
Bacillus cereus
NADH
-
Bacillus cereus
NADP+
-
Bacillus cereus
NADPH
-
Bacillus cereus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
is not inhibited by 1-propanol, lactate potassium, malate potassium, malonate potassium, acetaldehyde, ethanol, and glycerin
Bacillus cereus
Zn2+
inhibits at 0.2 mM
Bacillus cereus
ZnCl2
0.2 mM, 60% inhibition; inhibits approximately 60% of 3-HIBADH activity at 0.2 mM but shows no effect at 0.005 mM
Bacillus cereus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.25
-
NADP+
; pH 8.5, 37C
Bacillus cereus
2.4
-
NAD+
; pH 8.5, 37C
Bacillus cereus
16.8
-
3-hydroxypropionate
; pH 8.5, 37C, KM-value for 3-hydroxyisobutyrate is approximately 20fold lower
Bacillus cereus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
has no metal ion requirement, MnSO4, CuSO4, CaCl2, MgSO4, and FeSO4 have no effect; no metal ion requirement
Bacillus cereus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
gel filtration
Bacillus cereus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-hydroxy-2-methylpropanoate + NAD+
Bacillus cereus
-
2-methyl-3-oxopropanoate + NADH + H+
-
-
r
3-hydroxy-2-methylpropanoate + NAD+
Bacillus cereus ATCC 14579
-
2-methyl-3-oxopropanoate + NADH + H+
-
-
r
3-hydroxy-2-methylpropanoate + NAD+
Bacillus cereus ATCC14579
-
2-methyl-3-oxopropanoate + NADH + H+
-
-
r
3-hydroxy-2-methylpropanoate + NADP+
Bacillus cereus
-
2-methyl-3-oxopropanoate + NADPH + H+
-
-
r
3-hydroxy-2-methylpropanoate + NADP+
Bacillus cereus ATCC 14579
-
2-methyl-3-oxopropanoate + NADPH + H+
-
-
r
3-hydroxy-2-methylpropanoate + NADP+
Bacillus cereus ATCC14579
-
2-methyl-3-oxopropanoate + NADPH + H+
-
-
r
additional information
Bacillus cereus
MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59
?
-
-
-
additional information
Bacillus cereus ATCC 14579
MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59
?
-
-
-
additional information
Bacillus cereus ATCC14579
MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
96fold purified at 4C by ammonium sulfate fractionation and gel filtration; ammonium sulfate precipitation, hydrophobic interaction chromatography (Phenyl-Sepharose), anion exchange chromatography; native enzyme 100fold by ammonium sulfate fractionation, and hydrophobic interaction and anion exchange chromatography
Bacillus cereus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.091
-
homogenate
Bacillus cereus
8.7
-
purified enzyme
Bacillus cereus
8.77
-
pH 8.5, 37C; purified enzyme, pH 8.5, 37C
Bacillus cereus
Storage Stability (protein specific)
Storage Stability
Organism
-20C, 50 mM Tris-HCl buffer, pH 8.5, 2 mM EDTA, 2 mM DTT, 50% glycerol
Bacillus cereus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-hydroxy-2-methylpropanoate + NAD+
-
695710
Bacillus cereus
2-methyl-3-oxopropanoate + NADH + H+
-
-
-
r
3-hydroxy-2-methylpropanoate + NAD+
-
695710
Bacillus cereus ATCC 14579
2-methyl-3-oxopropanoate + NADH + H+
-
-
-
r
3-hydroxy-2-methylpropanoate + NAD+
-
695710
Bacillus cereus ATCC14579
2-methyl-3-oxopropanoate + NADH + H+
-
-
-
r
3-hydroxy-2-methylpropanoate + NADP+
-
695710
Bacillus cereus
2-methyl-3-oxopropanoate + NADPH + H+
-
-
-
r
3-hydroxy-2-methylpropanoate + NADP+
-
695710
Bacillus cereus ATCC 14579
2-methyl-3-oxopropanoate + NADPH + H+
-
-
-
r
3-hydroxy-2-methylpropanoate + NADP+
-
695710
Bacillus cereus ATCC14579
2-methyl-3-oxopropanoate + NADPH + H+
-
-
-
r
3-hydroxyisobutyrate + NAD+
-
695710
Bacillus cereus
methylmalonate semialdehyde + NADH + H+
-
-
-
r
3-hydroxypropanoate + NAD(P)+
is more active with NADP+ than NAD+
695710
Bacillus cereus
?
-
-
-
?
3-hydroxypropanoate + NAD(P)+
is more active with NADP+ than NAD+
695710
Bacillus cereus ATCC 14579
?
-
-
-
?
3-hydroxypropanoate + NAD(P)+
is more active with NADP+ than NAD+
695710
Bacillus cereus ATCC14579
?
-
-
-
?
3-hydroxypropanoate + NAD+
-
695710
Bacillus cereus
malonate semialdehyde + NADH + H+
-
-
-
r
3-hydroxypropanoate + NADP+
-
695710
Bacillus cereus
malonate semialdehyde + NADPH + H+
-
-
-
r
additional information
MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59
695710
Bacillus cereus
?
-
-
-
-
additional information
wide substrate specificity of the 3-HIBADH
695710
Bacillus cereus
?
-
-
-
-
additional information
MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59
695710
Bacillus cereus ATCC 14579
?
-
-
-
-
additional information
wide substrate specificity of the 3-HIBADH
695710
Bacillus cereus ATCC 14579
?
-
-
-
-
additional information
MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59
695710
Bacillus cereus ATCC14579
?
-
-
-
-
additional information
wide substrate specificity of the 3-HIBADH
695710
Bacillus cereus ATCC14579
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 32000, SDS-PAGE
Bacillus cereus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
; assay at
Bacillus cereus
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
35
45
shows more than 90% of its optimized activity
Bacillus cereus
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
45
-
enzyme activity is not decreased during preincubation at 45C within 30 min, but preincubation at 55C in 3 min can significantly denaturate and deactivate the enzyme; stable at 45C for 30 min, about 40% activity after 3 min at 55C
Bacillus cereus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.21
-
3-hydroxypropionate
; pH 8.5, 37C
Bacillus cereus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
assay at
Bacillus cereus
8.8
9
; oxidation of 3-hydroxypropionate by 3-HIBADH
Bacillus cereus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
10
; oxidation of 3-hydroxypropionate by 3-HIBADH
Bacillus cereus
General Information
General Information
Commentary
Organism
physiological function
3-hydroxyisobutyrate dehydrogenase is a key enzyme for the metabolism of valine and some keto-bodies. It can catalyze reversible conversion of 3-hydroxyisobutyrate to methylmalonate semialdehyde
Bacillus cereus
General Information (protein specific)
General Information
Commentary
Organism
physiological function
3-hydroxyisobutyrate dehydrogenase is a key enzyme for the metabolism of valine and some keto-bodies. It can catalyze reversible conversion of 3-hydroxyisobutyrate to methylmalonate semialdehyde
Bacillus cereus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0125
-
3-hydroxypropionate
pH 8.5, 37C
Bacillus cereus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0125
-
3-hydroxypropionate
pH 8.5, 37C
Bacillus cereus
Other publictions for EC 1.1.1.31
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737620
Park
Structural and biochemical cha ...
Bacillus cereus
Biochem. Biophys. Res. Commun.
474
522-527
2016
-
-
1
-
-
-
-
2
-
-
1
-
-
2
-
-
1
-
-
-
-
-
4
1
-
-
-
2
-
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
1
-
-
-
-
1
-
-
-
-
4
1
-
-
-
2
-
1
-
-
-
-
-
-
2
2
737911
Lee
-
3-Hydroxyisobutyrate dehydroge ...
Pseudomonas denitrificans, Pseudomonas denitrificans ATCC 13867
Biotechnol. Bioprocess Eng.
19
1-7
2014
3
-
1
-
-
-
11
5
-
2
-
-
-
3
-
-
1
-
-
-
2
-
11
1
1
1
-
5
1
1
-
1
-
-
-
3
-
1
1
-
-
-
-
11
-
5
-
2
-
-
-
-
-
1
-
-
2
-
11
1
1
1
-
5
1
1
-
-
-
-
-
-
5
5
722472
Tasi
Characterization of 3-hydroxyi ...
Homo sapiens
J. Assist. Reprod. Genet.
30
505-512
2013
-
-
-
-
-
-
-
-
2
-
-
1
-
3
-
-
-
-
-
11
-
-
1
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
11
-
-
1
-
1
-
-
-
1
-
-
-
2
3
3
2
-
-
739465
Zhou
Cloning, expression and charac ...
Pseudomonas denitrificans, Pseudomonas denitrificans ATCC 13867
PLoS ONE
8
e62666
2013
3
-
1
-
-
-
8
6
-
2
-
-
-
6
-
-
1
-
-
-
-
-
10
1
1
1
-
6
1
1
-
1
-
-
-
3
-
1
1
-
-
-
-
8
-
6
-
2
-
-
-
-
-
1
-
-
-
-
10
1
1
1
-
6
1
1
-
-
-
-
-
-
6
6
695710
Yao
The catalytic property of 3-hy ...
Bacillus cereus, Bacillus cereus ATCC 14579, Bacillus cereus ATCC14579
Appl. Biochem. Biotechnol.
160
694-703
2009
1
1
1
-
-
-
3
3
-
1
1
9
-
10
-
-
1
-
-
-
3
1
18
1
1
1
1
1
2
1
-
6
-
-
-
1
1
1
6
-
-
-
-
3
-
3
-
1
1
9
-
-
-
1
-
-
3
1
18
1
1
1
1
1
2
1
-
-
-
1
1
-
1
1
688515
Murin
Expression of 3-hydroxyisobuty ...
Rattus norvegicus
J. Neurochem.
105
1176-1186
2008
-
-
-
-
-
-
-
-
2
-
-
1
-
2
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670284
Loupatty
Clinical, biochemical, and mol ...
Homo sapiens
Mol. Genet. Metab.
87
243-248
2006
-
-
1
-
-
-
-
2
-
-
-
-
-
3
-
-
1
-
-
1
3
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
1
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655051
Chowdhury
Cloning and overexpression of ...
Pseudomonas putida, Pseudomonas putida E23
Biosci. Biotechnol. Biochem.
67
438-441
2003
-
-
1
-
-
-
-
-
-
-
2
-
-
4
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286550
Hawes
Mammalien 3-hydroxyisobutyrate ...
Homo sapiens, Oryctolagus cuniculus, Rattus norvegicus
Methods Enzymol.
324
218-228
2000
-
-
2
-
-
-
-
2
-
-
-
-
-
3
-
-
3
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
3
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286551
Chowdhury
3-Hydroxyisobutyrate dehydroge ...
Candida rugosa, Oryctolagus cuniculus, Pseudomonas putida, Pseudomonas putida E23
Biosci. Biotechnol. Biochem.
60
2043-2047
1996
-
-
-
-
-
-
7
10
-
-
4
3
-
7
-
-
1
-
-
1
2
-
10
3
-
-
-
-
3
-
3
3
-
-
-
-
-
-
3
-
-
-
-
7
-
10
-
-
4
3
-
-
-
1
-
1
2
-
10
3
-
-
-
-
3
-
3
-
-
-
-
-
-
-
286552
Hawes
Structural and mechanistic asp ...
Rattus norvegicus
Adv. Exp. Med. Biol.
414
395-402
1996
-
-
-
-
1
-
-
8
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
8
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286544
Rougraff
Purification and characterizat ...
Oryctolagus cuniculus
J. Biol. Chem.
263
327-331
1988
-
-
-
-
-
-
2
-
-
-
2
1
-
2
-
-
1
-
-
3
1
-
1
1
-
-
-
2
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
2
1
-
-
-
1
-
3
1
-
1
1
-
-
-
2
1
1
-
-
-
-
-
-
-
-
286523
Worral
3-Hydroxyisobutyrate dehydroge ...
Rhodobacter sphaeroides
Biochem. J.
241
297-300
1987
-
-
-
-
-
-
-
2
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286546
Hasegawa
-
Distribution in organisms and ...
Bos taurus, Candida parapsilosis, Candida rugosa, Corynebacterium hydrocarbons, Endomyces reessii, Micrococcus flavus, Micrococcus luteus, Nocardia lyena, Oryctolagus cuniculus, Pseudomonas aeruginosa, Pseudomonas dacunhae, Rhodotorula mucilaginosa, Sus scrofa, Trichosporon aculeatum, Tsukamurella paurometabola
Agric. Biol. Chem.
45
2899-2901
1981
-
-
-
-
-
-
-
-
-
-
-
16
-
15
-
-
1
-
-
4
-
-
16
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
16
-
-
-
1
-
4
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286547
Hasegawa
-
Purification, crystallization ...
Candida rugosa
Agric. Biol. Chem.
45
2805-2814
1981
-
-
-
1
-
-
-
3
-
-
2
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
3
-
-
2
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286548
De Paul Marshall
Regulation of valine catabolis ...
Pseudomonas putida
J. Bacteriol.
110
1073-1081
1972
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286549
Robinson
The purification and propertie ...
Sus scrofa
J. Biol. Chem.
225
511-521
1956
-
-
-
-
-
-
3
2
-
-
-
-
-
1
-
-
1
-
-
3
1
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
2
-
-
-
-
-
-
-
1
-
3
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-