BRENDA - Enzyme Database show
show all sequences of 1.1.1.31

Structural and mechanistic aspects of a new family of dehydrogenases, the beta-hydroxyacid dehydrogenases

Hawes, J.W.; Harper, E.T.; Crabb, D.W.; Harris, R.A.; Adv. Exp. Med. Biol. 414, 395-402 (1996)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
D33R
higher affinity to cofactor NADP+ than to natural cofactor NAD+
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.022
-
NAD+
wild type
Rattus norvegicus
0.056
-
L-3-hydroxyisobutyrate
wild type, NAD as cofactor
Rattus norvegicus
0.104
-
L-3-hydroxyisobutyrate
wild type, NADP as cofactor
Rattus norvegicus
0.11
-
L-3-hydroxyisobutyrate
mutant D33R, NADP as cofactor
Rattus norvegicus
0.112
-
L-3-hydroxyisobutyrate
mutant D33R, NAD as cofactor
Rattus norvegicus
0.24
-
NADP+
mutant D33R
Rattus norvegicus
0.96
-
NADP+
wild type
Rattus norvegicus
1.24
-
NAD+
mutant D33R
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-3-hydroxyisobutyrate + NAD+
Rattus norvegicus
-
L-2-methyl-3-oxopropionate + NADH
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-3-hydroxyisobutyrate + NAD+
-
286552
Rattus norvegicus
L-2-methyl-3-oxopropionate + NADH
-
-
-
?
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D33R
higher affinity to cofactor NADP+ than to natural cofactor NAD+
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.022
-
NAD+
wild type
Rattus norvegicus
0.056
-
L-3-hydroxyisobutyrate
wild type, NAD as cofactor
Rattus norvegicus
0.104
-
L-3-hydroxyisobutyrate
wild type, NADP as cofactor
Rattus norvegicus
0.11
-
L-3-hydroxyisobutyrate
mutant D33R, NADP as cofactor
Rattus norvegicus
0.112
-
L-3-hydroxyisobutyrate
mutant D33R, NAD as cofactor
Rattus norvegicus
0.24
-
NADP+
mutant D33R
Rattus norvegicus
0.96
-
NADP+
wild type
Rattus norvegicus
1.24
-
NAD+
mutant D33R
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-3-hydroxyisobutyrate + NAD+
Rattus norvegicus
-
L-2-methyl-3-oxopropionate + NADH
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-3-hydroxyisobutyrate + NAD+
-
286552
Rattus norvegicus
L-2-methyl-3-oxopropionate + NADH
-
-
-
?
Other publictions for EC 1.1.1.31
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737620
Park
Structural and biochemical cha ...
Bacillus cereus
Biochem. Biophys. Res. Commun.
474
522-527
2016
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1
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2
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1
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2
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1
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1
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1
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1
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1
1
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1
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4
1
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2
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1
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2
2
737911
Lee
-
3-Hydroxyisobutyrate dehydroge ...
Pseudomonas denitrificans, Pseudomonas denitrificans ATCC 13867
Biotechnol. Bioprocess Eng.
19
1-7
2014
3
-
1
-
-
-
11
5
-
2
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3
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1
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2
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11
1
1
1
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5
1
1
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1
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3
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1
1
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11
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5
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2
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1
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2
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11
1
1
1
-
5
1
1
-
-
-
-
-
-
5
5
722472
Tasi
Characterization of 3-hydroxyi ...
Homo sapiens
J. Assist. Reprod. Genet.
30
505-512
2013
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2
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2
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1
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-
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11
-
-
1
-
1
-
-
-
1
-
-
-
2
3
3
2
-
-
739465
Zhou
Cloning, expression and charac ...
Pseudomonas denitrificans, Pseudomonas denitrificans ATCC 13867
PLoS ONE
8
e62666
2013
3
-
1
-
-
-
8
6
-
2
-
-
-
6
-
-
1
-
-
-
-
-
10
1
1
1
-
6
1
1
-
1
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-
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3
-
1
1
-
-
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-
8
-
6
-
2
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-
-
-
1
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-
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10
1
1
1
-
6
1
1
-
-
-
-
-
-
6
6
695710
Yao
The catalytic property of 3-hy ...
Bacillus cereus, Bacillus cereus ATCC 14579, Bacillus cereus ATCC14579
Appl. Biochem. Biotechnol.
160
694-703
2009
1
1
1
-
-
-
3
3
-
1
1
9
-
10
-
-
1
-
-
-
3
1
18
1
1
1
1
1
2
1
-
6
-
-
-
1
1
1
6
-
-
-
-
3
-
3
-
1
1
9
-
-
-
1
-
-
3
1
18
1
1
1
1
1
2
1
-
-
-
1
1
-
1
1
688515
Murin
Expression of 3-hydroxyisobuty ...
Rattus norvegicus
J. Neurochem.
105
1176-1186
2008
-
-
-
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-
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2
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1
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2
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5
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1
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2
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1
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5
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1
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-
-
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-
-
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-
-
-
-
670284
Loupatty
Clinical, biochemical, and mol ...
Homo sapiens
Mol. Genet. Metab.
87
243-248
2006
-
-
1
-
-
-
-
2
-
-
-
-
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3
-
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1
-
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1
3
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1
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1
-
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1
1
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-
-
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2
-
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-
1
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1
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655051
Chowdhury
Cloning and overexpression of ...
Pseudomonas putida, Pseudomonas putida E23
Biosci. Biotechnol. Biochem.
67
438-441
2003
-
-
1
-
-
-
-
-
-
-
2
-
-
4
-
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1
-
-
-
-
-
2
1
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1
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1
1
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2
-
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-
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1
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-
-
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2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286550
Hawes
Mammalien 3-hydroxyisobutyrate ...
Homo sapiens, Oryctolagus cuniculus, Rattus norvegicus
Methods Enzymol.
324
218-228
2000
-
-
2
-
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2
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3
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3
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1
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2
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2
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2
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3
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1
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2
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-
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-
286551
Chowdhury
3-Hydroxyisobutyrate dehydroge ...
Candida rugosa, Oryctolagus cuniculus, Pseudomonas putida, Pseudomonas putida E23
Biosci. Biotechnol. Biochem.
60
2043-2047
1996
-
-
-
-
-
-
7
10
-
-
4
3
-
7
-
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1
-
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1
2
-
10
3
-
-
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3
-
3
3
-
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-
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3
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-
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7
-
10
-
-
4
3
-
-
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1
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1
2
-
10
3
-
-
-
-
3
-
3
-
-
-
-
-
-
-
286552
Hawes
Structural and mechanistic asp ...
Rattus norvegicus
Adv. Exp. Med. Biol.
414
395-402
1996
-
-
-
-
1
-
-
8
-
-
-
1
-
1
-
-
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-
-
-
-
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1
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1
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8
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1
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1
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286544
Rougraff
Purification and characterizat ...
Oryctolagus cuniculus
J. Biol. Chem.
263
327-331
1988
-
-
-
-
-
-
2
-
-
-
2
1
-
2
-
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1
-
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3
1
-
1
1
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-
-
2
1
1
-
1
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-
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1
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-
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2
-
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2
1
-
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1
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3
1
-
1
1
-
-
-
2
1
1
-
-
-
-
-
-
-
-
286523
Worral
3-Hydroxyisobutyrate dehydroge ...
Rhodobacter sphaeroides
Biochem. J.
241
297-300
1987
-
-
-
-
-
-
-
2
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1
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4
-
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1
1
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1
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1
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1
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2
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1
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1
1
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1
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286546
Hasegawa
-
Distribution in organisms and ...
Bos taurus, Candida parapsilosis, Candida rugosa, Corynebacterium hydrocarbons, Endomyces reessii, Micrococcus flavus, Micrococcus luteus, Nocardia lyena, Oryctolagus cuniculus, Pseudomonas aeruginosa, Pseudomonas dacunhae, Rhodotorula mucilaginosa, Sus scrofa, Trichosporon aculeatum, Tsukamurella paurometabola
Agric. Biol. Chem.
45
2899-2901
1981
-
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16
-
15
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1
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4
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16
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3
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3
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16
-
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1
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4
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16
-
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286547
Hasegawa
-
Purification, crystallization ...
Candida rugosa
Agric. Biol. Chem.
45
2805-2814
1981
-
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1
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3
-
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2
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1
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1
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1
1
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3
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1
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1
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286548
De Paul Marshall
Regulation of valine catabolis ...
Pseudomonas putida
J. Bacteriol.
110
1073-1081
1972
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286549
Robinson
The purification and propertie ...
Sus scrofa
J. Biol. Chem.
225
511-521
1956
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3
2
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1
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1
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1
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1
1
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