Literature summary for 1.1.1.307 extracted from

Suzuki, T.; Yokoyama, S.; Kinoshita, Y.; Yamada, H.; Hatsu, M.; Takamizawa, K.; Kawai, K.
Expression of xyrA gene encoding for D-xylose reductase of Candida tropicalis and production of xylitol in Escherichia coli (1999), J. Biosci. Bioeng., 87, 280-284 .

Search for more literature for 1.1.1.307

Application

Application	Comment	Organism
synthesis	Escherichia coli expressing XylA synthesizes 13.3 g/l of xylitol during 20 h of cultivation, when D-xylose (50 g/l) and D-glucose (5 g/l) are added to IPTG-induced cells	Candida tropicalis

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Candida tropicalis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0091	-	NADPH	recombinant enzyme, pH not specified in the publication, temperature not specified in the publication	Candida tropicalis
0.0141	-	NADPH	native enzyme, pH not specified in the publication, temperature not specified in the publication	Candida tropicalis
26.9	-	D-xylose	native enzyme, pH not specified in the publication, temperature not specified in the publication	Candida tropicalis
38.2	-	D-xylose	recombinant enzyme, pH not specified in the publication, temperature not specified in the publication	Candida tropicalis

Organism

Organism	UniProt	Comment	Textmining
Candida tropicalis	O13283	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-galactose + NADH + H+	-	Candida tropicalis	? + NAD+	-	?
D-glucose + NADH + H+	-	Candida tropicalis	? + NAD+	-	?
D-xylose + NADPH + H+	-	Candida tropicalis	xylitol + NADP+	-	?
DL-glyceraldehyde + NADH + H+	-	Candida tropicalis	xylitol + NAD+	-	?
L-arabinose + NADPH + H+	-	Candida tropicalis	? + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
XyrA	-	Candida tropicalis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Candida tropicalis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Candida tropicalis

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Release 2023.1 (January 2023)