Crystallization (Comment) | Organism |
---|---|
structures of enzyme bound to NADP+ and NAD+ show two different protein conformations capable of accommodating the presence or absence of the coenzyme 2'-phosphate group | Yamadazyma tenuis |
Protein Variants | Comment | Organism |
---|---|---|
K274G | mutant shows an increase in NADH versus NADPH selectivity with only modest alterations of the original NADH-linked xylose specificity and catalytic-centre activity | Yamadazyma tenuis |
K274M | mutant shows an increase in NADH versus NADPH selectivity with only modest alterations of the original NADH-linked xylose specificity and catalytic-centre activity | Yamadazyma tenuis |
K274R | mutant shows an increase in NADH versus NADPH selectivity with only modest alterations of the original NADH-linked xylose specificity and catalytic-centre activity | Yamadazyma tenuis |
K274R/N276D | mutant shows an increase in NADH versus NADPH selectivity with only modest alterations of the original NADH-linked xylose specificity and catalytic-centre activity. Mutant exhibits a 5fold preference for NADH over NADPH | Yamadazyma tenuis |
N276D | mutant shows an increase in NADH versus NADPH selectivity with only modest alterations of the original NADH-linked xylose specificity and catalytic-centre activity | Yamadazyma tenuis |
R280H | mutant shows an increase in NADH versus NADPH selectivity with only modest alterations of the original NADH-linked xylose specificity and catalytic-centre activity | Yamadazyma tenuis |
S275A | mutant shows an increase in NADH versus NADPH selectivity with only modest alterations of the original NADH-linked xylose specificity and catalytic-centre activity | Yamadazyma tenuis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yamadazyma tenuis | O74237 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NADH + H+ | - |
Yamadazyma tenuis | xylitol + NAD+ | - |
? | |
D-xylose + NADPH + H+ | - |
Yamadazyma tenuis | xylitol + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AKR2B5 | - |
Yamadazyma tenuis |
XYL1 | - |
Yamadazyma tenuis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | 6 | NADPH | mutant K274M, pH 7, 25°C | Yamadazyma tenuis | |
8 | - |
NADH | mutant K274R, pH 7, 25°C | Yamadazyma tenuis | |
10 | - |
NADPH | mutant K274G, pH 7, 25°C | Yamadazyma tenuis | |
11 | - |
NADH | wild-type, pH 7, 25°C | Yamadazyma tenuis | |
12 | - |
NADH | mutant K274R/N276D, pH 7, 25°C | Yamadazyma tenuis | |
13 | - |
NADPH | wild-type, pH 7, 25°C | Yamadazyma tenuis | |
14 | - |
NADH | mutant N276D, pH 7, 25°C | Yamadazyma tenuis | |
15 | - |
NADH | mutant K274G, pH 7, 25°C | Yamadazyma tenuis | |
15 | - |
NADPH | mutant R280H , pH 7, 25°C | Yamadazyma tenuis | |
19 | - |
NADH | mutant K274M, pH 7, 25°C | Yamadazyma tenuis | |
21 | - |
NADH | mutant R280H , pH 7, 25°C | Yamadazyma tenuis | |
24 | - |
NADH | mutant S275A, pH 7, 25°C | Yamadazyma tenuis | |
26 | - |
NADPH | mutant K274R, pH 7, 25°C | Yamadazyma tenuis | |
29 | - |
NADPH | mutant S275A, pH 7, 25°C | Yamadazyma tenuis | |
30 | - |
NADPH | mutant K274R/N276D, pH 7, 25°C | Yamadazyma tenuis | |
37 | - |
NADPH | mutant N276D, pH 7, 25°C | Yamadazyma tenuis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | NADPH is preferred approx. 33fold over NADH | Yamadazyma tenuis | |
NADPH | NADPH is preferred approx. 33fold over NADH | Yamadazyma tenuis |