Cloned (Comment) | Organism |
---|---|
recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Thermochaetoides thermophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Thermochaetoides thermophila | |
0.119 | - |
NADH | recombinant enzyme, pH 6.5, 30°C, with D-xylose | Thermochaetoides thermophila | |
0.135 | - |
NADPH | recombinant enzyme, pH 6.5, 30°C, with D-xylose | Thermochaetoides thermophila | |
22.3 | - |
D-xylose | recombinant enzyme, pH 6.5, 30°C | Thermochaetoides thermophila | |
25.4 | - |
D-xylose | recombinant enzyme, pH 6.5, 55°C | Thermochaetoides thermophila | |
62.5 | - |
L-arabinose | recombinant enzyme, pH 6.5, 30°C, with NADPH | Thermochaetoides thermophila | |
241 | - |
D-galactose | recombinant enzyme, pH 6.5, 30°C, with NADPH | Thermochaetoides thermophila | |
471 | - |
D-glucose | recombinant enzyme, pH 6.5, 30°C, with NADPH | Thermochaetoides thermophila |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Thermochaetoides thermophila | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NADPH + H+ | Thermochaetoides thermophila | - |
xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | Thermochaetoides thermophila IMI 039719 | - |
xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | Thermochaetoides thermophila DSM 1495 | - |
xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | Thermochaetoides thermophila CBS 144.50 | - |
xylitol + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermochaetoides thermophila | G0SCE7 | - |
- |
Thermochaetoides thermophila CBS 144.50 | G0SCE7 | - |
- |
Thermochaetoides thermophila DSM 1495 | G0SCE7 | - |
- |
Thermochaetoides thermophila IMI 039719 | G0SCE7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme 25.5fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration to near homogeneity | Thermochaetoides thermophila |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | optimal growth temperature of the organism is 50-55°C | Thermochaetoides thermophila | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + NADPH + H+ | - |
Thermochaetoides thermophila | galactitol + NADP+ | - |
r | |
D-galactose + NADPH + H+ | - |
Thermochaetoides thermophila IMI 039719 | galactitol + NADP+ | - |
r | |
D-galactose + NADPH + H+ | - |
Thermochaetoides thermophila DSM 1495 | galactitol + NADP+ | - |
r | |
D-galactose + NADPH + H+ | - |
Thermochaetoides thermophila CBS 144.50 | galactitol + NADP+ | - |
r | |
D-glucose + NADPH + H+ | - |
Thermochaetoides thermophila | glucitol + NADP+ | - |
r | |
D-ribose + NADPH + H+ | - |
Thermochaetoides thermophila | ribitol + NADP+ | - |
r | |
D-xylose + NADH + H+ | - |
Thermochaetoides thermophila | xylitol + NAD+ | - |
r | |
D-xylose + NADH + H+ | - |
Thermochaetoides thermophila IMI 039719 | xylitol + NAD+ | - |
r | |
D-xylose + NADH + H+ | - |
Thermochaetoides thermophila DSM 1495 | xylitol + NAD+ | - |
r | |
D-xylose + NADH + H+ | - |
Thermochaetoides thermophila CBS 144.50 | xylitol + NAD+ | - |
r | |
D-xylose + NADPH + H+ | - |
Thermochaetoides thermophila | xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | best substrates | Thermochaetoides thermophila | xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | - |
Thermochaetoides thermophila IMI 039719 | xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | best substrates | Thermochaetoides thermophila IMI 039719 | xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | - |
Thermochaetoides thermophila DSM 1495 | xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | best substrates | Thermochaetoides thermophila DSM 1495 | xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | - |
Thermochaetoides thermophila CBS 144.50 | xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | best substrates | Thermochaetoides thermophila CBS 144.50 | xylitol + NADP+ | - |
r | |
L-arabinose + NADPH + H+ | - |
Thermochaetoides thermophila | arabitol + NADP+ | - |
r | |
additional information | the enzyme exhibits dual cofactor specificity for NADPH and NADH and prefers D-xylose over other pentoses and investigated hexoses. No or poor activity with D-lyxose, D-mannose, L-xylose, and D-arabinose. L-arabinose, being structurally similar to D-xylose, has a kcat/KM value of still 33% of the value for D-xylose, whereas D-galactose and D-glucose result in only 6.3% and 1.3% of the kcat/KM for D-xylose. The difference in the catalytic efficiency is mainly a result from different KM values rather than differences in kcat | Thermochaetoides thermophila | ? | - |
- |
|
additional information | the enzyme exhibits dual cofactor specificity for NADPH and NADH and prefers D-xylose over other pentoses and investigated hexoses. No or poor activity with D-lyxose, D-mannose, L-xylose, and D-arabinose. L-arabinose, being structurally similar to D-xylose, has a kcat/KM value of still 33% of the value for D-xylose, whereas D-galactose and D-glucose result in only 6.3% and 1.3% of the kcat/KM for D-xylose. The difference in the catalytic efficiency is mainly a result from different KM values rather than differences in kcat | Thermochaetoides thermophila IMI 039719 | ? | - |
- |
|
additional information | the enzyme exhibits dual cofactor specificity for NADPH and NADH and prefers D-xylose over other pentoses and investigated hexoses. No or poor activity with D-lyxose, D-mannose, L-xylose, and D-arabinose. L-arabinose, being structurally similar to D-xylose, has a kcat/KM value of still 33% of the value for D-xylose, whereas D-galactose and D-glucose result in only 6.3% and 1.3% of the kcat/KM for D-xylose. The difference in the catalytic efficiency is mainly a result from different KM values rather than differences in kcat | Thermochaetoides thermophila DSM 1495 | ? | - |
- |
|
additional information | the enzyme exhibits dual cofactor specificity for NADPH and NADH and prefers D-xylose over other pentoses and investigated hexoses. No or poor activity with D-lyxose, D-mannose, L-xylose, and D-arabinose. L-arabinose, being structurally similar to D-xylose, has a kcat/KM value of still 33% of the value for D-xylose, whereas D-galactose and D-glucose result in only 6.3% and 1.3% of the kcat/KM for D-xylose. The difference in the catalytic efficiency is mainly a result from different KM values rather than differences in kcat | Thermochaetoides thermophila CBS 144.50 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 39244, sequence calculation, x * 41000, recombinant His-tagged enzyme, SDS-PAGE | Thermochaetoides thermophila |
Synonyms | Comment | Organism |
---|---|---|
CTHT_0056950 | - |
Thermochaetoides thermophila |
CtXR | - |
Thermochaetoides thermophila |
NAD(P)H-dependent D-xylose reductase-like protein | UniProt | Thermochaetoides thermophila |
xylose reductase | - |
Thermochaetoides thermophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
higher activity at 55°C compared to 30°C | Thermochaetoides thermophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
purified recombinant His-tagged enzyme, no loss of activity after 3 h at 30°C | Thermochaetoides thermophila |
55 | 75 | purified recombinant His-tagged enzyme, half-lifes are 1.8 min at 55°C, 4.2 s at 65°C, and 2.8 s at 75°C, respectively | Thermochaetoides thermophila |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
D-glucose | recombinant enzyme, pH 6.5, 30°C, with NADPH | Thermochaetoides thermophila | |
3.2 | - |
NADH | recombinant enzyme, pH 6.5, 30°C, with D-xylose | Thermochaetoides thermophila | |
6.5 | - |
D-galactose | recombinant enzyme, pH 6.5, 30°C, with NADPH | Thermochaetoides thermophila | |
8.8 | - |
L-arabinose | recombinant enzyme, pH 6.5, 30°C, with NADPH | Thermochaetoides thermophila | |
9.2 | - |
D-xylose | recombinant enzyme, pH 6.5, 30°C, with NADPH | Thermochaetoides thermophila | |
11.4 | - |
NADPH | recombinant enzyme, pH 6.5, 30°C, with D-xylose | Thermochaetoides thermophila | |
35.2 | - |
D-xylose | recombinant enzyme, pH 6.5, 55°C, with NADPH | Thermochaetoides thermophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | in 50 mM citrate and 50 mM phosphate buffer | Thermochaetoides thermophila |
7 | - |
in a citrate-disodium phosphate buffer | Thermochaetoides thermophila |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | over 40% of maximal activity within this range, profile overview | Thermochaetoides thermophila |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme exhibits dual cofactor specificity for NADPH and NADH, the catalytic efficiency is significantly higher for NADPH | Thermochaetoides thermophila | |
NADP+ | - |
Thermochaetoides thermophila | |
NADPH | modeled cofactor-binding site in CtXR, overview | Thermochaetoides thermophila |
General Information | Comment | Organism |
---|---|---|
additional information | structure homology modeling based on a XR structure from Candida sp. (CaXR) as template (PDB ID 1SM9), analysis of the architecture of the cofactor binding site. Notable CaXR-to-CtXR replacements include N276T, L277R, R280I and Q283S | Thermochaetoides thermophila |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
D-glucose | recombinant enzyme, pH 6.5, 30°C, with NADPH | Thermochaetoides thermophila | |
0.027 | - |
D-galactose | recombinant enzyme, pH 6.5, 30°C, with NADPH | Thermochaetoides thermophila | |
0.141 | - |
L-arabinose | recombinant enzyme, pH 6.5, 30°C, with NADPH | Thermochaetoides thermophila | |
0.413 | - |
D-xylose | recombinant enzyme, pH 6.5, 30°C | Thermochaetoides thermophila | |
1.386 | - |
D-xylose | recombinant enzyme, pH 6.5, 55°C | Thermochaetoides thermophila | |
26.9 | - |
NADH | recombinant enzyme, pH 6.5, 30°C, with D-xylose | Thermochaetoides thermophila | |
84.4 | - |
NADPH | recombinant enzyme, pH 6.5, 30°C, with D-xylose | Thermochaetoides thermophila |