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Literature summary for 1.1.1.307 extracted from

  • Woodyer, R.; Simurdiak, M.; van der Donk, W.A.; Zhao, H.
    Heterologous expression, purification, and characterization of a highly active xylose reductase from Neurospora crassa (2005), Appl. Environ. Microbiol., 71, 1642-1647.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin 1 mg/ml, 30% activation Neurospora crassa
EDTA 1 mM, 5% activation Neurospora crassa

Application

Application Comment Organism
synthesis this enzyme is one of the most active xylose reductases and may be used for the in vitro production of xylitol Neurospora crassa

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as a His6-tagged fusion protein in high yield Neurospora crassa

Inhibitors

Inhibitors Comment Organism Structure
additional information no effect 0.1% w/v sodium azide Neurospora crassa
NaCl 50 mM, 25% inhibition Neurospora crassa
sodium phosphite 200 mM, 37% inhibition Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
NADPH pH 6.3, 25°C Neurospora crassa
0.016
-
NADH pH 6.3, 25°C Neurospora crassa
34
-
D-xylose pH 6.3, 25°C, cosubstrate: NADPH Neurospora crassa
37
-
D-xylose pH 6.3, 25°C, cosubstrate: NADH Neurospora crassa
40
-
L-arabinose pH 6.3, 25°C Neurospora crassa
70
-
D-ribose pH 6.3, 25°C Neurospora crassa
180
-
D-galactose pH 6.3, 25°C Neurospora crassa
360
-
D-glucose pH 6.3, 25°C Neurospora crassa

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no effect: 1 mM CuSO4 Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38400
-
2 * 38400, SDS-PAGE Neurospora crassa
53000
-
gel filtration Neurospora crassa

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Neurospora crassa

Storage Stability

Storage Stability Organism
4°C, stable for several months Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose + NADPH + H+ catalytic efficiency is 9.1% of the catalytic efficiency for D-xylose Neurospora crassa ?
-
?
D-glucose + NADPH + H+ catalytic efficiency is 3.3% of the catalytic efficiency for D-xylose Neurospora crassa ?
-
?
D-ribose + NADPH + H+ catalytic efficiency is 41% of the catalytic efficiency for D-xylose Neurospora crassa ?
-
?
D-xylose + NADH + H+ catalytic efficiency for NADPH is more than 100fold higher than the catalytic efficiency for NADH Neurospora crassa xylitol + NAD+
-
?
D-xylose + NADPH + H+ catalytic efficiency for NADPH is more than 100fold higher than the catalytic efficiency for NADH Neurospora crassa xylitol + NADP+
-
?
L-arabinose + NADPH + H+ catalytic efficiency is 41% of the catalytic efficiency for D-xylose Neurospora crassa L-arabinitol + NADP+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 38400, SDS-PAGE Neurospora crassa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 55
-
Neurospora crassa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 65 25°C: about 55% of maximal activity, 65°C: about 65% of maximal activity Neurospora crassa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
21
-
at room temperature stable for more than 1 month Neurospora crassa
40
-
half-life: 94 min Neurospora crassa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
310
-
NADH pH 6.3, 25°C Neurospora crassa
310
-
D-xylose pH 6.3, 25°C, cosubstrate: NADH Neurospora crassa
1320
-
D-glucose pH 6.3, 25°C Neurospora crassa
1800
-
D-galactose pH 6.3, 25°C Neurospora crassa
1800
-
L-arabinose pH 6.3, 25°C Neurospora crassa
3120
-
D-ribose pH 6.3, 25°C Neurospora crassa
3600
-
NADPH pH 6.3, 25°C Neurospora crassa
3600
-
D-xylose pH 6.3, 25°C, cosubstrate: NADPH Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Neurospora crassa

pH Range

pH Minimum pH Maximum Comment Organism
4 7 pH 4.0: about 40% of maximal activity, pH 7.0: about 50% of maximal activity Neurospora crassa

Cofactor

Cofactor Comment Organism Structure
NADH catalytic efficiency for NADPH is more than 100fold higher than the catalytic efficiency for NADH Neurospora crassa
NADPH catalytic efficiency for NADPH is more than 100fold higher than the catalytic efficiency for NADH Neurospora crassa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.05
-
D-glucose pH 6.3, 25°C Neurospora crassa
0.16
-
D-galactose pH 6.3, 25°C Neurospora crassa
0.75
-
L-arabinose pH 6.3, 25°C Neurospora crassa
0.75
-
D-ribose pH 6.3, 25°C Neurospora crassa
1.8
-
D-xylose pH 6.3, 25°C, cosubstrate: NADPH Neurospora crassa
33.3
-
NADPH pH 6.3, 25°C Neurospora crassa