Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus licheniformis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, 0.5% of initial activity with substrate diacetyl, 0.5% with substrate 2,3-butanediol, respectively | Bacillus licheniformis | |
Al3+ | 1 mM, 4% of initial activity with substrate diacetyl, 6% with substrate 2,3-butanediol, respectively | Bacillus licheniformis | |
Cu2+ | 1 mM, 0.2% of initial activity with substrate diacetyl, 1% with substrate 2,3-butanediol, respectively | Bacillus licheniformis | |
EDTA | 1 mM, 91% of initial activity with substrate diacetyl, 90% with substrate 2,3-butanediol, respectively | Bacillus licheniformis | |
Fe3+ | 1 mM, 2% of initial activity with substrate diacetyl, 3.5% with substrate 2,3-butanediol, respectively | Bacillus licheniformis | |
Zn2+ | 1 mM, 75% of initial activity with substrate diacetyl, 80% with substrate 2,3-butanediol, respectively | Bacillus licheniformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
NADH | pH 6.0, 30°C | Bacillus licheniformis | |
0.34 | - |
NAD+ | pH 10.0, 30°C | Bacillus licheniformis | |
0.47 | - |
(3S)-acetoin | pH 6.0, 30°C | Bacillus licheniformis | |
7.25 | - |
(2S,3S)-2,3-butanediol | pH 10.0, 30°C | Bacillus licheniformis | |
72.4 | - |
diacetyl | pH 6.0, 30°C | Bacillus licheniformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
4 * 30000, SDS-PAGE | Bacillus licheniformis |
125000 | - |
gel filtration | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
120 | - |
substrate diacetyl, 30°C, pH 6.0 | Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,3S)-2,3-butanediol + NAD+ | - |
Bacillus licheniformis | (3S)-acetoin + NADH + H+ | - |
r | |
(3S)-acetoin + NADH + H+ | 97% of the activity with diacetyl | Bacillus licheniformis | (2S,3S)-2,3-butanediol + NAD+ | 97.3% enantiomeric excess and 96.5% diastereomeric excess | r | |
1,2-propanediol + NAD+ | - |
Bacillus licheniformis | ? + NADH + H+ | 0.5% of the activity with 2,3-butanediol | r | |
2,3-hexanedione + NADH + H+ | - |
Bacillus licheniformis | ? + NAD+ | 66% of the activity with diacetyl | ? | |
2,3-pentanedione + NADH + H+ | - |
Bacillus licheniformis | ? + NAD+ | 69% of the activity with diacetyl | ? | |
3,4-hexanedione + NADH + H+ | - |
Bacillus licheniformis | ? + NAD+ | 10% of the activity with diacetyl | ? | |
diacetyl + NADH + H+ | - |
Bacillus licheniformis | (3S)-acetoin + NAD+ | 97.3% enantiomeric excess | ir | |
additional information | enzyme shows oxidative activity to racemic 2,3-butanediol but no activity toward racemic acetoin in the presence of NAD+ | Bacillus licheniformis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 30000, SDS-PAGE | Bacillus licheniformis |
Synonyms | Comment | Organism |
---|---|---|
budC | - |
Bacillus licheniformis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
202 | - |
(3S)-acetoin | pH 6.0, 30°C | Bacillus licheniformis | |
591 | - |
(2S,3S)-2,3-butanediol | pH 10.0, 30°C | Bacillus licheniformis | |
748 | - |
NAD+ | pH 10.0, 30°C | Bacillus licheniformis | |
1222 | - |
diacetyl | pH 6.0, 30°C | Bacillus licheniformis | |
1274 | - |
NADH | pH 6.0, 30°C | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
reduction of diacetyl | Bacillus licheniformis |
10 | - |
oxidation of butanediol | Bacillus licheniformis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | reduction of diacetyl | Bacillus licheniformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADPH | Bacillus licheniformis | |
NAD+ | - |
Bacillus licheniformis | |
NADH | - |
Bacillus licheniformis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.9 | - |
diacetyl | pH 6.0, 30°C | Bacillus licheniformis | |
81.5 | - |
(2S,3S)-2,3-butanediol | pH 10.0, 30°C | Bacillus licheniformis | |
432 | - |
(3S)-acetoin | pH 6.0, 30°C | Bacillus licheniformis | |
2192 | - |
NAD+ | pH 10.0, 30°C | Bacillus licheniformis | |
5072 | - |
NADH | pH 6.0, 30°C | Bacillus licheniformis |