Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aquifex aeolicus |
expression in Escherichia coli | [Candida] glabrata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25300 | - |
2 * 25300, SDS-PAGE | Aquifex aeolicus |
28500 | - |
2 * 28500, SDS-PAGE | [Candida] glabrata |
50600 | - |
sedimentation equilibrium analysis | Aquifex aeolicus |
57800 | - |
sedimentation equilibrium analysis | [Candida] glabrata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | Methanocaldococcus jannaschii | biosynthesis of riboflavin | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | Aquifex aeolicus | biosynthesis of riboflavin | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | [Candida] glabrata | biosynthesis of riboflavin | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | Methanocaldococcus jannaschii DSM 2661 | biosynthesis of riboflavin | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | [Candida] glabrata ATCC 2001 | biosynthesis of riboflavin | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | O66747 | - |
- |
Methanocaldococcus jannaschii | Q58085 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | Q58085 | - |
- |
[Candida] glabrata | Q6FU96 | - |
- |
[Candida] glabrata ATCC 2001 | Q6FU96 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Aquifex aeolicus |
- |
[Candida] glabrata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | biosynthesis of riboflavin | Methanocaldococcus jannaschii | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | biosynthesis of riboflavin | Aquifex aeolicus | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | biosynthesis of riboflavin | [Candida] glabrata | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation | Aquifex aeolicus | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation. The enzyme is an A-type reductase catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1' (S) position of the substrate | Methanocaldococcus jannaschii | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation. The enzyme is an A-type reductase catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1' (S) position of the substrate | [Candida] glabrata | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | biosynthesis of riboflavin | Methanocaldococcus jannaschii DSM 2661 | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation. The enzyme is an A-type reductase catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1' (S) position of the substrate | Methanocaldococcus jannaschii DSM 2661 | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | biosynthesis of riboflavin | [Candida] glabrata ATCC 2001 | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation. The enzyme is an A-type reductase catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1' (S) position of the substrate | [Candida] glabrata ATCC 2001 | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 28500, SDS-PAGE | [Candida] glabrata |
homodimer | 2 * 25300, SDS-PAGE | Aquifex aeolicus |
Synonyms | Comment | Organism |
---|---|---|
2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase | - |
Methanocaldococcus jannaschii |
2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase | - |
Aquifex aeolicus |
2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase | - |
[Candida] glabrata |
arfC | - |
Methanocaldococcus jannaschii |
MJ0671 | locus name | Methanocaldococcus jannaschii |
ribD2 | - |
Aquifex aeolicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanocaldococcus jannaschii |
37 | - |
assay at | Aquifex aeolicus |
37 | - |
assay at | [Candida] glabrata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
assay at | Methanocaldococcus jannaschii |
8.2 | - |
assay at | [Candida] glabrata |
8.2 | - |
asay at | Aquifex aeolicus |
General Information | Comment | Organism |
---|---|---|
physiological function | biosynthesis of riboflavin | Methanocaldococcus jannaschii |
physiological function | biosynthesis of riboflavin | Aquifex aeolicus |
physiological function | biosynthesis of riboflavin | [Candida] glabrata |