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show all sequences of 1.1.1.30

Characterization of a novel 3-hydroxybutyrate dehydrogenase from Ralstonia pickettii T1

Takanashi, M.; Shiraki, M.; Saito, T.; Antonie van Leeuwenhoek 95, 249-262 (2009)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
BDH1 is abundant in cells grown on D-fructose, D-glucose, maltose, oxaloacetate, 2-oxoglutarate, L-alanine, L-arginine, L-methionine, poly-3-hydroxybutyrate, and nutrient broth, and poor in cells grown on pyruvate, gluconate, fumarate, L-malate, propionate, and n-butyrate; BDH2 is abundant in cells grown on acetate, ethanol, D-arabinose, D-ribose, oxaloacetate, 2-oxoglutarate, malaete, L-histidine, L-alanine, L-arginine, L-methionine, and poly-3-hydroxybutyrate, and scant in cells grown on pyruvate, n-butyrate, and oleate; BDH3 is produced only in cells grown on 3-hydroxybutyrate or poly-3-hydroxybutyrate as a carbon source. Expression of bdh3 may be coordinately regulated with a gene encoding putative 3-hydroxybutyrate permease
Ralstonia pickettii
Cloned(Commentary)
Commentary
Organism
BDH3 expressed in Escherichia coli BLR (DE3)/pLysS harboring pETT13
Ralstonia pickettii
Engineering
Amino acid exchange
Commentary
Organism
additional information
the bdh1 mutant lags behind the wild-type in growth rates when the cells are cultured with 3-hydroxybutyrate, citrate, succinate, or nutrient broth. A test of sensitivity to diamide as an oxidative stress reveals that the lack of BDH1 causes a decline in the capacity to neutralize the stress; the bdh2 mutant lags behind the wild-type in growth rates when the cells are cultured with 3-hydroxybutyrate, citrate, succinate, or nutrient broth. A test of sensitivity to diamide as an oxidative stress reveals that the lack of BDH2 causes a decline in the capacity to neutralize the stress
Ralstonia pickettii
Inhibitors
Inhibitors
Commentary
Organism
Structure
Diamide
-
Ralstonia pickettii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.064
-
NAD+
-
Ralstonia pickettii
0.1
-
NAD+
-
Ralstonia pickettii
0.24
-
NADH
-
Ralstonia pickettii
0.25
-
acetoacetate
-
Ralstonia pickettii
0.31
-
NAD+
-
Ralstonia pickettii
0.32
-
NADH
-
Ralstonia pickettii
0.34
-
D-3-hydroxybutyrate
-
Ralstonia pickettii
0.49
-
D-3-hydroxybutyrate
-
Ralstonia pickettii
0.5
-
D-3-hydroxybutyrate
-
Ralstonia pickettii
1.1
-
acetoacetate
-
Ralstonia pickettii
1.1
-
NADH
-
Ralstonia pickettii
1.3
-
acetoacetate
-
Ralstonia pickettii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
x * 28000, SDS-PAGE, BDH3
Ralstonia pickettii
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ralstonia pickettii
A8R3J3
T1
-
Ralstonia pickettii
Q2PEN2
T1
-
Ralstonia pickettii
Q2PEN3
T1
-
Ralstonia pickettii T1
A8R3J3
T1
-
Ralstonia pickettii T1
Q2PEN2
T1
-
Ralstonia pickettii T1
Q2PEN3
T1
-
Purification (Commentary)
Commentary
Organism
BDH3 partially purified from bdh2 mutant by three steps of column chromatography. Gene product of bdh3 expressed in Escherichia coli purified with 100% yield by two steps of column chromatography
Ralstonia pickettii
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1200
-
purified BDH3
Ralstonia pickettii
Storage Stability
Storage Stability
Organism
-20░C, 20 mM phosphate buffer, pH 7.0, 20% glycerol
Ralstonia pickettii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-3-hydroxybutyrate + NAD+
BDH1 is needed to regulate the cytoplasmic redox state as well as to utilize 3-hydroxybutyrate
695653
Ralstonia pickettii
acetoacetate + NADH + H+
-
-
-
r
D-3-hydroxybutyrate + NAD+
BDH2 is needed to regulate the cytoplasmic redox state as well as to utilize 3-hydroxybutyrate
695653
Ralstonia pickettii
acetoacetate + NADH + H+
-
-
-
r
D-3-hydroxybutyrate + NAD+
BDH3 is specialized to utilize 3-hydroxybutyrate
695653
Ralstonia pickettii
acetoacetate + NADH + H+
-
-
-
r
D-3-hydroxybutyrate + NAD+
BDH3 is specialized to utilize 3-hydroxybutyrate
695653
Ralstonia pickettii T1
acetoacetate + NADH + H+
-
-
-
r
D-3-hydroxybutyrate + NAD+
BDH2 is needed to regulate the cytoplasmic redox state as well as to utilize 3-hydroxybutyrate
695653
Ralstonia pickettii T1
acetoacetate + NADH + H+
-
-
-
r
D-3-hydroxybutyrate + NAD+
BDH1 is needed to regulate the cytoplasmic redox state as well as to utilize 3-hydroxybutyrate
695653
Ralstonia pickettii T1
acetoacetate + NADH + H+
-
-
-
r
poly-3-hydroxybutyrate + NAD+
-
695653
Ralstonia pickettii
acetoacetate + NADH + H+
-
-
-
r
poly-3-hydroxybutyrate + NAD+
-
695653
Ralstonia pickettii T1
acetoacetate + NADH + H+
-
-
-
r
Subunits
Subunits
Commentary
Organism
?
x * 28000, SDS-PAGE, BDH3
Ralstonia pickettii
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
58
-
NADH
-
Ralstonia pickettii
87
-
NAD+
-
Ralstonia pickettii
100
-
acetoacetate
-
Ralstonia pickettii
110
-
acetoacetate
-
Ralstonia pickettii
150
-
acetoacetate
-
Ralstonia pickettii
180
-
D-3-hydroxybutyrate
-
Ralstonia pickettii
280
-
NADH
-
Ralstonia pickettii
310
-
D-3-hydroxybutyrate
-
Ralstonia pickettii
340
-
NAD+
-
Ralstonia pickettii
360
-
NAD+
-
Ralstonia pickettii
500
-
D-3-hydroxybutyrate
-
Ralstonia pickettii
740
-
NADH
-
Ralstonia pickettii
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
8.5
optimal pH of BDH3 is 8.5 in the oxidation reaction and 6.5 in the reduction reaction
Ralstonia pickettii
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
BDH1 is abundant in cells grown on D-fructose, D-glucose, maltose, oxaloacetate, 2-oxoglutarate, L-alanine, L-arginine, L-methionine, poly-3-hydroxybutyrate, and nutrient broth, and poor in cells grown on pyruvate, gluconate, fumarate, L-malate, propionate, and n-butyrate
Ralstonia pickettii
additional information
BDH2 is abundant in cells grown on acetate, ethanol, D-arabinose, D-ribose, oxaloacetate, 2-oxoglutarate, malaete, L-histidine, L-alanine, L-arginine, L-methionine, and poly-3-hydroxybutyrate, and scant in cells grown on pyruvate, n-butyrate, and oleate
Ralstonia pickettii
additional information
BDH3 is produced only in cells grown on 3-hydroxybutyrate or poly-3-hydroxybutyrate as a carbon source. Expression of bdh3 may be coordinately regulated with a gene encoding putative 3-hydroxybutyrate permease
Ralstonia pickettii
Cloned(Commentary) (protein specific)
Commentary
Organism
BDH3 expressed in Escherichia coli BLR (DE3)/pLysS harboring pETT13
Ralstonia pickettii
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
the bdh1 mutant lags behind the wild-type in growth rates when the cells are cultured with 3-hydroxybutyrate, citrate, succinate, or nutrient broth. A test of sensitivity to diamide as an oxidative stress reveals that the lack of BDH1 causes a decline in the capacity to neutralize the stress
Ralstonia pickettii
additional information
the bdh2 mutant lags behind the wild-type in growth rates when the cells are cultured with 3-hydroxybutyrate, citrate, succinate, or nutrient broth. A test of sensitivity to diamide as an oxidative stress reveals that the lack of BDH2 causes a decline in the capacity to neutralize the stress
Ralstonia pickettii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Diamide
-
Ralstonia pickettii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.064
-
NAD+
-
Ralstonia pickettii
0.1
-
NAD+
-
Ralstonia pickettii
0.24
-
NADH
-
Ralstonia pickettii
0.25
-
acetoacetate
-
Ralstonia pickettii
0.31
-
NAD+
-
Ralstonia pickettii
0.32
-
NADH
-
Ralstonia pickettii
0.34
-
D-3-hydroxybutyrate
-
Ralstonia pickettii
0.49
-
D-3-hydroxybutyrate
-
Ralstonia pickettii
0.5
-
D-3-hydroxybutyrate
-
Ralstonia pickettii
1.1
-
acetoacetate
-
Ralstonia pickettii
1.1
-
NADH
-
Ralstonia pickettii
1.3
-
acetoacetate
-
Ralstonia pickettii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
x * 28000, SDS-PAGE, BDH3
Ralstonia pickettii
Purification (Commentary) (protein specific)
Commentary
Organism
BDH3 partially purified from bdh2 mutant by three steps of column chromatography. Gene product of bdh3 expressed in Escherichia coli purified with 100% yield by two steps of column chromatography
Ralstonia pickettii
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1200
-
purified BDH3
Ralstonia pickettii
Storage Stability (protein specific)
Storage Stability
Organism
-20░C, 20 mM phosphate buffer, pH 7.0, 20% glycerol
Ralstonia pickettii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-3-hydroxybutyrate + NAD+
BDH1 is needed to regulate the cytoplasmic redox state as well as to utilize 3-hydroxybutyrate
695653
Ralstonia pickettii
acetoacetate + NADH + H+
-
-
-
r
D-3-hydroxybutyrate + NAD+
BDH2 is needed to regulate the cytoplasmic redox state as well as to utilize 3-hydroxybutyrate
695653
Ralstonia pickettii
acetoacetate + NADH + H+
-
-
-
r
D-3-hydroxybutyrate + NAD+
BDH3 is specialized to utilize 3-hydroxybutyrate
695653
Ralstonia pickettii
acetoacetate + NADH + H+
-
-
-
r
D-3-hydroxybutyrate + NAD+
BDH3 is specialized to utilize 3-hydroxybutyrate
695653
Ralstonia pickettii T1
acetoacetate + NADH + H+
-
-
-
r
D-3-hydroxybutyrate + NAD+
BDH2 is needed to regulate the cytoplasmic redox state as well as to utilize 3-hydroxybutyrate
695653
Ralstonia pickettii T1
acetoacetate + NADH + H+
-
-
-
r
D-3-hydroxybutyrate + NAD+
BDH1 is needed to regulate the cytoplasmic redox state as well as to utilize 3-hydroxybutyrate
695653
Ralstonia pickettii T1
acetoacetate + NADH + H+
-
-
-
r
poly-3-hydroxybutyrate + NAD+
-
695653
Ralstonia pickettii
acetoacetate + NADH + H+
-
-
-
r
poly-3-hydroxybutyrate + NAD+
-
695653
Ralstonia pickettii T1
acetoacetate + NADH + H+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 28000, SDS-PAGE, BDH3
Ralstonia pickettii
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
58
-
NADH
-
Ralstonia pickettii
87
-
NAD+
-
Ralstonia pickettii
100
-
acetoacetate
-
Ralstonia pickettii
110
-
acetoacetate
-
Ralstonia pickettii
150
-
acetoacetate
-
Ralstonia pickettii
180
-
D-3-hydroxybutyrate
-
Ralstonia pickettii
280
-
NADH
-
Ralstonia pickettii
310
-
D-3-hydroxybutyrate
-
Ralstonia pickettii
340
-
NAD+
-
Ralstonia pickettii
360
-
NAD+
-
Ralstonia pickettii
500
-
D-3-hydroxybutyrate
-
Ralstonia pickettii
740
-
NADH
-
Ralstonia pickettii
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
8.5
optimal pH of BDH3 is 8.5 in the oxidation reaction and 6.5 in the reduction reaction
Ralstonia pickettii
Other publictions for EC 1.1.1.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737394
Kanazawa
Structural insights into the c ...
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Acta Crystallogr. Sect. F
72
507-515
2016
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1
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2
-
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1
-
2
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1
-
-
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1
1
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1
-
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1
1
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2
-
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1
-
-
-
1
-
-
-
-
1
1
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-
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737914
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Structural basis for the subst ...
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1
-
15
-
-
15
-
-
-
1
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
15
-
-
-
1
-
-
-
-
-
1
1
-
15
-
-
-
-
15
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
15
-
-
-
-
-
-
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-
13
13
739558
Davuluri
Inactivation of 3-hydroxybutyr ...
Danio rerio
Proc. Natl. Acad. Sci. USA
113
E1460-E1469
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-
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-
-
-
-
-
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-
-
1
-
3
-
-
-
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-
2
-
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1
-
-
-
-
-
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1
-
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-
1
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-
-
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-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
737467
Scaturro
Identification and characteriz ...
Legionella pneumophila subsp. pneumophila, Legionella pneumophila subsp. pneumophila DSM 7513
APMIS
123
330-341
2015
-
-
-
-
-
-
-
-
1
-
-
2
-
6
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
729048
Lu
3-Hydroxybutyrate oligomer hyd ...
Paracoccus denitrificans, Paracoccus denitrificans ATCC 17741
Appl. Environ. Microbiol.
80
986-993
2014
-
-
1
-
-
-
-
-
1
-
1
2
-
4
-
-
1
-
-
-
-
-
4
1
-
-
-
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-
1
-
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1
1
-
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1
-
1
2
-
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1
-
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4
1
-
-
-
-
-
-
-
-
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-
-
-
-
737912
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-
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Alcaligenes faecalis
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19
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-
-
1
-
3
2
-
8
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
8
-
-
-
1
-
-
-
-
-
1
1
-
3
2
-
-
-
8
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
8
-
-
-
-
-
-
-
-
8
8
738207
Liu
3-Hydroxybutyrate dehydrogenas ...
Mus musculus
FEBS J.
281
2410-2421
2014
-
-
-
-
-
-
-
-
-
-
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-
-
4
-
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1
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1
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1
1
-
-
-
721853
Yeon
Enzymatic reduction of levulin ...
Alcaligenes faecalis
Biores. Technol.
134
377-380
2013
-
1
1
-
1
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
1
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
711807
Mountassif
Structural and catalytic prope ...
Pseudomonas aeruginosa
Curr. Microbiol.
61
7-12
2010
-
-
1
-
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
-
-
-
-
2
-
-
-
-
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-
-
1
-
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-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695376
Hoque
Structure of D-3-hydroxybutyra ...
Alcaligenes faecalis
Acta Crystallogr. Sect. F
65
331-335
2009
-
-
1
1
-
-
-
-
-
-
-
-
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2
-
-
1
-
-
-
-
-
1
1
-
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1
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2
-
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-
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-
1
2
1
-
-
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1
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1
1
-
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-
-
1
-
-
-
-
-
-
-
-
-
695653
Takanashi
Characterization of a novel 3- ...
Ralstonia pickettii, Ralstonia pickettii T1
Antonie van Leeuwenhoek
95
249-262
2009
1
-
1
-
1
-
1
12
-
-
1
-
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9
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1
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1
1
12
1
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12
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1
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3
-
1
-
-
2
-
-
3
-
12
-
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1
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1
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1
1
12
1
-
-
-
12
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1
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698660
Nakashima
Closed complex of the D-3-hydr ...
Pseudomonas fragi
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2009
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1
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24
1
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1
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1
22
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1
-
47
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1
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1
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-
24
1
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-
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684149
Hoque
The structures of Alcaligenes ...
Alcaligenes faecalis
Acta Crystallogr. Sect. D
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1
1
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1
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1
1
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-
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689351
Mountassif
-
Biochemical and histological a ...
Jaculus orientalis
Pestic. Biochem. Physiol.
90
87-96
2008
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-
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1
2
1
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1
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1
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2
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1
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2
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1
-
1
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-
-
1
-
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679792
Paithankar
Cosubstrate-induced dynamics o ...
Pseudomonas putida
FEBS J.
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2007
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1
1
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3
1
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4
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2
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2
3
2
4
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4
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1
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1
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-
3
1
-
-
-
4
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-
-
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680512
Uchino
Isolated poly(3-hydroxybutyrat ...
Cupriavidus necator
J. Bacteriol.
189
8250-8256
2007
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1
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1
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1
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3
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1
3
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1
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2
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1
-
-
-
1
-
-
-
-
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-
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685409
Mountassif
Prehibernation and hibernation ...
Jaculus orientalis
Biochimie
89
1019-1028
2007
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1
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2
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1
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1
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2
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2
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1
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1
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1
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1
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2
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1
-
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2
-
2
-
-
-
1
-
-
-
-
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-
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668251
Feller
Molecular basis of substrate r ...
Pseudomonas putida
ChemBioChem
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1410-1418
2006
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1
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5
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10
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2
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1
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1
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5
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1
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1
1
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5
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10
-
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1
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1
-
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-
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5
-
-
-
-
-
-
-
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668322
Mountassif
-
Characterization of two D-b-hy ...
Jaculus orientalis
Comp. Biochem. Physiol. B
143B
285-293
2006
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-
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2
4
1
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1
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1
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3
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2
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1
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1
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1
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2
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4
1
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1
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3
-
2
-
-
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1
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669434
Guo
Characterization of human DHRS ...
Homo sapiens
J. Biol. Chem.
281
10291-10297
2006
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1
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1
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1
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1
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1
1
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4
2
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1
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1
-
-
1
-
-
-
-
-
-
-
-
-
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669544
Takanashi
Characterization of two 3-hydr ...
Ralstonia pickettii, Ralstonia pickettii T1
J. Biosci. Bioeng.
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501-507
2006
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1
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1
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4
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4
1
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8
4
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2
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2
4
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-
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8
-
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4
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2
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4
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4
2
-
-
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8
4
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669873
Ito
D-3-hydroxybutyrate dehydrogen ...
Pseudomonas fragi
J. Mol. Biol.
355
722-733
2006
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1
1
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5
4
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1
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3
1
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-
-
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2
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-
2
5
-
-
-
-
1
2
1
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-
-
5
5
4
-
-
1
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-
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1
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-
-
-
3
1
-
-
-
-
2
-
-
-
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-
667070
Nakajima
Crystallization and preliminar ...
Pseudomonas fragi
Acta Crystallogr. Sect. F
61
36-38
2005
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1
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1
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-
-
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-
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-
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656326
Takanashi
Biochemical and genetic charac ...
Acidovorax sp.
J. Biosci. Bioeng.
97
78-81
2004
5
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1
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4
4
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2
1
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5
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1
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2
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2
1
1
-
1
-
2
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-
2
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-
-
5
-
1
2
-
-
-
-
4
-
4
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3
2
1
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-
-
1
-
-
2
-
2
1
1
-
1
-
2
-
-
-
-
-
-
-
-
-
655126
Kabine
Hibernation impact on the cata ...
Jaculus orientalis
BMC Biochem.
4
11
2003
-
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-
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9
2
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-
2
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2
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3
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-
3
-
1
1
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
9
2
-
-
2
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-
-
-
-
3
-
-
3
-
1
1
-
-
1
-
-
-
-
-
-
-
-
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655261
Nasser
Purification and characterizat ...
Camelus dromedarius
Comp. Biochem. Physiol. B
131
9-18
2002
1
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-
-
-
-
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5
3
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1
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3
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1
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2
1
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1
1
1
1
3
-
2
-
-
2
-
-
-
1
-
-
2
-
-
-
-
-
-
5
3
-
1
-
-
-
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1
-
2
1
-
1
1
1
1
3
-
2
-
-
-
-
-
-
-
-
-
286512
Loeb-Hennard
-
(R)-3-Hydroxybutyrate dehydrog ...
Homo sapiens
Biochemistry
39
11929-11938
2000
1
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1
-
-
-
-
-
2
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1
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1
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1
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1
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1
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1
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1
1
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2
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1
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-
1
-
-
1
-
-
-
-
-
-
-
-
-
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-
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286513
Green
Wild type and mutant human hea ...
Bos taurus, Homo sapiens
Biochemistry
35
8158-8165
1996
2
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1
-
3
-
-
6
4
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2
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2
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2
7
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2
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2
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2
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1
2
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3
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6
4
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2
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-
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2
7
-
2
-
-
-
-
-
-
-
-
-
-
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-
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286514
Klein
Specific interaction of(R)-3-h ...
Bos taurus
Biochemistry
35
3044-3049
1996
1
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-
-
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2
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1
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1
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1
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1
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1
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2
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1
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1
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1
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-
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286515
Szewczyk
Occurence, purification and pr ...
Staphylococcus xylosus
Acta Microbiol. Pol.
43
33-45
1994
1
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4
2
6
3
1
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1
1
1
1
1
1
1
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2
1
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1
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1
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1
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4
2
6
3
1
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1
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1
1
1
1
1
1
1
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2
1
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286516
Adam
Monoclonal antibodies for stru ...
Bos taurus, Rattus norvegicus
Biochem. J.
292
863-872
1993
2
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3
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3
4
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2
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2
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2
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2
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3
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2
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3
4
-
2
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-
-
-
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-
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286517
Dalton
Effect of selective thiol-grou ...
Bos taurus
Biochem. J.
296
563-569
1993
1
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1
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1
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4
1
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1
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1
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-
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-
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286518
Dutra
Inhibition of succinate dehydr ...
Rattus norvegicus
J. Inherit. Metab. Dis.
16
147-153
1993
1
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2
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1
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1
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1
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1
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4
2
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1
-
-
-
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286519
Marks
Molecular cloning and characte ...
Homo sapiens
J. Biol. Chem.
267
15459-15463
1992
1
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3
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1
1
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1
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1
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1
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1
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2
1
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3
-
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1
1
-
-
-
-
-
-
-
-
-
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-
-
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286520
Bailly
Post-transcriptional analysis ...
Rattus norvegicus
Biol. Cell.
73
121-129
1991
1
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-
-
-
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1
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1
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2
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4
3
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1
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1
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1
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1
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1
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1
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4
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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286521
McIntyre
Comparison of 3-hydroxybutyrat ...
Bos taurus, Rattus norvegicus
Arch. Biochem. Biophys.
262
85-98
1988
2
-
-
-
-
-
-
4
4
-
4
2
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4
-
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2
-
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7
2
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2
2
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-
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-
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2
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2
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2
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4
4
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4
2
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2
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7
2
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
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286522
Cortese
Noncooperative vs. cooperative ...
Bos taurus, Rattus norvegicus
Biochemistry
26
5283-5293
1987
2
-
-
-
-
-
-
-
2
-
-
2
-
3
-
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-
-
-
-
-
2
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-
-
-
-
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2
-
-
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2
-
-
2
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286523
Worral
3-Hydroxyisobutyrate dehydroge ...
Rhodobacter sphaeroides
Biochem. J.
241
297-300
1987
1
-
-
-
-
-
-
-
-
-
-
1
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4
-
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-
-
-
-
1
-
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1
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1
-
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1
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1
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-
1
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-
-
-
-
-
-
-
-
-
1
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-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
286524
Kovar
-
Kinetics of D-3-hydroxybutyrat ...
Paracoccus denitrificans
Biochim. Biophys. Acta
871
302-309
1986
1
-
-
-
-
-
7
-
-
-
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1
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1
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-
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1
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2
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1
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1
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1
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7
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1
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-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
286525
Dubois
Cyanylation of 3-hydroxybutyra ...
Bos taurus
Biol. Chem. Hoppe-Seyler
367
343-353
1986
1
-
-
-
-
-
2
8
1
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1
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1
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1
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1
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1
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1
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1
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2
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8
1
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1
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1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286526
Matyskova I.; Kovar
-
Purification and properties of ...
Paracoccus denitrificans
Biochim. Biophys. Acta
839
300-307
1985
1
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2
1
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1
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1
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1
1
1
1
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2
1
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1
1
1
1
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286527
Burnett
A rapid and efficient procedur ...
Bos taurus
Biochim. Biophys. Acta
815
51-56
1985
1
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1
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1
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286529
MacGill
-
D-3-Hydroxybutyrate dehydrogen ...
Rattus norvegicus
Biochem. Soc. Trans.
607
788
1984
1
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1
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1
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1
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286530
McIntyre
Target size of D-beta-hydroxyb ...
Bos taurus
J. Biol. Chem.
258
953-959
1983
1
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1
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2
1
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2
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1
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286531
Scawen
The rapid purification of 3-hy ...
Rhodobacter sphaeroides
Biochem. J.
203
699-705
1982
1
-
-
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2
1
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1
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1
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1
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1
1
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286528
Phelps
Inhibition of D(-)-beta-hydrox ...
Bos taurus
Biochemistry
20
459-463
1981
1
-
-
-
-
-
4
1
1
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-
1
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2
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1
1
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2
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1
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1
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4
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1
1
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1
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1
1
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2
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-
-
-
-
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-
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-
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286532
Kluger
Inhibition of bacterial D-3-hy ...
Paucimonas lemoignei
Can. J. Biochem.
59
810-815
1981
1
-
-
-
-
-
8
-
-
-
-
1
-
1
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-
-
-
-
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1
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1
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1
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1
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8
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1
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1
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286533
Miyahara
Selective interaction of D-bet ...
Rattus norvegicus
Biochim. Biophys. Acta
641
222-231
1981
1
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1
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2
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1
2
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1
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1
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1
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2
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1
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-
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-
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286534
Nakada T.; Fukui
Purification and properties of ...
Zoogloea ramigera I-16-M
J. Biochem.
89
625-635
1981
6
-
-
-
-
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17
4
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2
2
1
-
2
-
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1
-
-
-
1
1
1
1
-
-
-
-
1
1
-
1
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6
-
-
1
-
-
-
-
17
-
4
-
2
2
1
-
-
-
1
-
-
1
1
1
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
286535
Phelps
Inhibition of D(-)-beta-hydrox ...
Bos taurus
Biochemistry
20
453-458
1981
1
-
-
-
-
-
6
4
1
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1
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1
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-
1
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1
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-
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1
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1
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1
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6
-
4
1
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1
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1
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1
-
-
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-
-
-
-
-
-
-
-
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-
-
-
286536
Tucker
The kinetics of rat liver and ...
Rattus norvegicus
Biochem. J.
179
579-581
1979
1
-
-
-
-
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8
1
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1
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3
-
-
-
-
-
4
-
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1
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-
-
-
-
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1
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1
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1
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-
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8
1
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1
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4
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1
-
-
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-
-
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-
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286537
Kluger
-
Substrate analogue studies of ...
Paucimonas lemoignei
J. Am. Chem. Soc.
100
7388-7392
1978
1
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-
-
10
-
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1
-
1
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3
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1
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1
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1
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1
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10
-
-
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1
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-
3
-
-
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-
-
1
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-
-
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286538
McIntyre
The orientation of D-beta-hydr ...
Bos taurus
Biochim. Biophys. Acta
513
255-267
1978
1
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2
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1
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2
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2
1
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1
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1
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1
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1
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2
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1
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2
1
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1
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-
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-
286539
Dhariwal
Purification and properties of ...
Mycobacterium phlei
J. Gen. Microbiol.
104
123-126
1978
1
-
-
-
-
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2
2
-
-
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1
-
2
-
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1
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1
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1
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1
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2
1
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1
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1
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1
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2
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2
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1
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1
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1
-
1
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1
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2
1
-
-
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-
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-
286540
Tan
Inhibition of D(-)-3-hydroxybu ...
Bos taurus, Cavia porcellus, Rattus norvegicus
Arch. Biochem. Biophys.
166
164-173
1975
3
-
-
-
-
-
20
-
-
-
-
3
-
3
-
-
-
-
-
3
-
-
3
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-
3
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3
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3
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20
-
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3
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3
-
-
3
-
-
-
-
-
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-
-
-
-
-
-
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286541
Thompson
Characterization and partial p ...
Bacillus cereus, Bacillus cereus T
Can. J. Microbiol.
19
673-677
1973
1
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-
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-
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2
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1
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2
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8
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1
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1
1
2
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1
1
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1
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1
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2
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1
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1
1
2
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1
1
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286542
Nielsen
Mitochondrial D-beta-hydroxybu ...
Bos taurus
J. Biol. Chem.
248
2549-2555
1973
1
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-
-
-
-
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1
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1
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1
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1
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1
1
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1
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1
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1
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1
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1
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1
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1
1
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1
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286543
Nielsen
Mitochondrial D-beta-hydroxybu ...
Bos taurus
J. Biol. Chem.
248
2556-2562
1973
1
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-
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5
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1
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1
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-
-
-
-
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1
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1
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1
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1
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5
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1
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1
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