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Literature summary for 1.1.1.30 extracted from
- Characterization and partial purification of beta-hydroxybutyrate dehydrogenase from sporulating cells of Bacillus cereus T (1973), Can. J. Microbiol., 19, 673-677.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphatidylcholine | - |
Bacillus cereus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.95 | - |
NAD+ | - |
Bacillus cereus | |
| 2.3 | - |
(R)-3-hydroxybutanoate | - |
Bacillus cereus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 0.1 mM, 2fold increase in activity, crude extracts | Bacillus cereus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-3-hydroxybutanoate + NAD+ | Bacillus cereus | - |
acetoacetate + NADH | - |
r | |
| (R)-3-hydroxybutanoate + NAD+ | Bacillus cereus T | - |
acetoacetate + NADH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
- |
- |
| Bacillus cereus T | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE-Sephadex, Sephadex G-200 | Bacillus cereus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 21 | - |
- |
Bacillus cereus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, Tris-HCl buffer, 50-100 mM Mg2+, Mn2+, Ba2+, or Ca2+, enzyme loses all activity in the presence of phosphate buffer or EDTA within 2 min | Bacillus cereus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-3-hydroxybutanoate + NAD+ | - |
Bacillus cereus | acetoacetate + NADH | - |
r | |
| (R)-3-hydroxybutanoate + NAD+ | - |
Bacillus cereus T | acetoacetate + NADH | - |
r | |
| (R)-3-hydroxybutanoate + NAD+ | - |
Bacillus cereus | acetoacetate + NADH + H+ | - |
r | |
| (R)-3-hydroxybutanoate + NAD+ | - |
Bacillus cereus T | acetoacetate + NADH + H+ | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bacillus cereus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Bacillus cereus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Bacillus cereus | |
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