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Literature summary for 1.1.1.30 extracted from
- Purification and properties of beta-hydroxybutyrate dehydrogenase from Mycobacterium phlei ATCC354 (1978), J. Gen. Microbiol., 104, 123-126.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphatidylcholine | - |
Mycolicibacterium phlei |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| HgCl2 | enzyme extremely sensitive, NADH and Ca2+ protect | Mycolicibacterium phlei | |
| p-chloromercuribenzoate | enzyme extremely sensitive, NADH and Ca2+ protect | Mycolicibacterium phlei | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.66 | - |
NAD+ | - |
Mycolicibacterium phlei | |
| 7.4 | - |
(R)-3-hydroxybutanoate | - |
Mycolicibacterium phlei | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-3-hydroxybutanoate + NAD+ | Mycolicibacterium phlei | - |
acetoacetate + NADH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium phlei | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE-cellulose | Mycolicibacterium phlei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14 | - |
- |
Mycolicibacterium phlei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-3-hydroxybutanoate + NAD+ | - |
Mycolicibacterium phlei | acetoacetate + NADH | - |
r | |
| (R)-3-hydroxybutanoate + NAD+ | - |
Mycolicibacterium phlei | acetoacetate + NADH + H+ | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
loss of 70% activity after 15 min, 0.24 mg protein/ml, Ca2+ and Mn2+ stabilize enzyme | Mycolicibacterium phlei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | 7 | reduction of acetoacetate | Mycolicibacterium phlei |
| 8.4 | - |
oxidation of beta-hydroxybutyrate | Mycolicibacterium phlei |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | 9 | oxidation of beta-hydroxybutyrate | Mycolicibacterium phlei |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Mycolicibacterium phlei | |
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