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Literature summary for 1.1.1.29 extracted from

  • Chistoserdova, L.V.; Lidstrom, M.E.
    Purification and characterization of hydroxypyruvate reductase from the facultative methylotroph Methylobacterium extorquens AM1 (1991), J. Bacteriol., 173, 7228-7232.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
Hydroxypyruvate product inhibition at a concentration above 4 mM, 99% inhibition of the reverse reaction at 0.5 mM Methylorubrum extorquens
NADH concentration above 0.5 mM Methylorubrum extorquens
NADPH concentration above 0.5 mM Methylorubrum extorquens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
NADH
-
Methylorubrum extorquens
0.06
-
NADPH
-
Methylorubrum extorquens
0.1
-
Hydroxypyruvate
-
Methylorubrum extorquens
0.12
-
Hydroxypyruvate
-
Methylorubrum extorquens
1.5
-
glyoxylate
-
Methylorubrum extorquens
2.6
-
glycerate
-
Methylorubrum extorquens
20
-
glyoxylate
-
Methylorubrum extorquens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
2 * 37000, SDS-PAGE Methylorubrum extorquens
71000
-
SDS-PAGE Methylorubrum extorquens

Organism

Organism UniProt Comment Textmining
Methylorubrum extorquens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methylorubrum extorquens

Storage Stability

Storage Stability Organism
4°C, 50 mM Tris-HCl buffer, pH 7.5, containing 10% glycerol and 1 mM dithiothreitol, 2 months, at least 90% of inital activity Methylorubrum extorquens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glycerate + NAD+
-
Methylorubrum extorquens hydroxypyruvate + NADH + H+
-
r
glyoxylate + NADH
-
Methylorubrum extorquens glycolate + NAD+
-
ir
hydroxypyruvate + NADH
-
Methylorubrum extorquens D-glycerate + NAD+
-
r
hydroxypyruvate + NADPH + H+
-
Methylorubrum extorquens D-glycerate + NADP+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 37000, SDS-PAGE Methylorubrum extorquens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 6.5 reduction of hydroxypyruvate Methylorubrum extorquens
9 11 glycerate oxidation Methylorubrum extorquens

Cofactor

Cofactor Comment Organism Structure
NADH
-
Methylorubrum extorquens
NADPH
-
Methylorubrum extorquens