BRENDA - Enzyme Database show
show all sequences of 1.1.1.29

Purification and characterization of hydroxypyruvate reductase from the facultative methylotroph Methylobacterium extorquens AM1

Chistoserdova, L.V.; Lidstrom, M.E.; J. Bacteriol. 173, 7228-7232 (1991)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Hydroxypyruvate
product inhibition at a concentration above 4 mM, 99% inhibition of the reverse reaction at 0.5 mM
Methylobacterium extorquens
NADH
concentration above 0.5 mM
Methylobacterium extorquens
NADPH
concentration above 0.5 mM
Methylobacterium extorquens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
NADH
-
Methylobacterium extorquens
0.06
-
NADPH
-
Methylobacterium extorquens
0.1
-
Hydroxypyruvate
-
Methylobacterium extorquens
0.12
-
Hydroxypyruvate
-
Methylobacterium extorquens
1.5
-
glyoxylate
-
Methylobacterium extorquens
2.6
-
glycerate
-
Methylobacterium extorquens
20
-
glyoxylate
-
Methylobacterium extorquens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
2 * 37000, SDS-PAGE
Methylobacterium extorquens
71000
-
SDS-PAGE
Methylobacterium extorquens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methylobacterium extorquens
-
-
-
Purification (Commentary)
Commentary
Organism
-
Methylobacterium extorquens
Storage Stability
Storage Stability
Organism
4°C, 50 mM Tris-HCl buffer, pH 7.5, containing 10% glycerol and 1 mM dithiothreitol, 2 months, at least 90% of inital activity
Methylobacterium extorquens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glyoxylate + NADH
-
286508
Methylobacterium extorquens
glycolate + NAD+
-
-
-
ir
hydroxypyruvate + NADH
-
286508
Methylobacterium extorquens
D-glycerate + NAD+
-
-
-
r
Subunits
Subunits
Commentary
Organism
dimer
2 * 37000, SDS-PAGE
Methylobacterium extorquens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4
6.5
reduction of hydroxypyruvate
Methylobacterium extorquens
9
11
glycerate oxidation
Methylobacterium extorquens
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Methylobacterium extorquens
NADPH
-
Methylobacterium extorquens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Methylobacterium extorquens
NADPH
-
Methylobacterium extorquens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Hydroxypyruvate
product inhibition at a concentration above 4 mM, 99% inhibition of the reverse reaction at 0.5 mM
Methylobacterium extorquens
NADH
concentration above 0.5 mM
Methylobacterium extorquens
NADPH
concentration above 0.5 mM
Methylobacterium extorquens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
NADH
-
Methylobacterium extorquens
0.06
-
NADPH
-
Methylobacterium extorquens
0.1
-
Hydroxypyruvate
-
Methylobacterium extorquens
0.12
-
Hydroxypyruvate
-
Methylobacterium extorquens
1.5
-
glyoxylate
-
Methylobacterium extorquens
2.6
-
glycerate
-
Methylobacterium extorquens
20
-
glyoxylate
-
Methylobacterium extorquens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
2 * 37000, SDS-PAGE
Methylobacterium extorquens
71000
-
SDS-PAGE
Methylobacterium extorquens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Methylobacterium extorquens
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 50 mM Tris-HCl buffer, pH 7.5, containing 10% glycerol and 1 mM dithiothreitol, 2 months, at least 90% of inital activity
Methylobacterium extorquens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glyoxylate + NADH
-
286508
Methylobacterium extorquens
glycolate + NAD+
-
-
-
ir
hydroxypyruvate + NADH
-
286508
Methylobacterium extorquens
D-glycerate + NAD+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 37000, SDS-PAGE
Methylobacterium extorquens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4
6.5
reduction of hydroxypyruvate
Methylobacterium extorquens
9
11
glycerate oxidation
Methylobacterium extorquens
Other publictions for EC 1.1.1.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Biochim. Biophys. Acta
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737745
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Arabidopsis thaliana
Biochim. Biophys. Acta
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739241
Pan
Glyoxylate reductase/hydroxypy ...
Homo sapiens
Pathobiology
80
155-162
2013
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726102
Cousins
Peroxisomal hydroxypyruvate re ...
Arabidopsis thaliana
Photosyn. Res.
108
91-100
2011
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728436
Kern
Evolution of enzymes involved ...
Arabidopsis thaliana
Photosyn. Res.
109
103-114
2011
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1
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286509
Julliard
Identification of hydroxypyruv ...
Petroselinum crispum
Protein Expr. Purif.
9
10-14
1997
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2
1
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2
1
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1
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1
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286510
Hagishita
Immunological characterization ...
Hyphomicrobium methylovorum
FEMS Microbiol. Lett.
142
49-52
1996
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286505
Kleczkowski
Oxalate as a potent and select ...
Hordeum vulgare, Zea mays
Biochem. J.
276
125-127
1991
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286508
Chistoserdova
Purification and characterizat ...
Methylobacterium extorquens
J. Bacteriol.
173
7228-7232
1991
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3
7
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2
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7
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2
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286506
Izumi
Purification and characterizat ...
Hyphomicrobium methylovorum
Eur. J. Biochem.
190
279-284
1990
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8
3
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2
1
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1
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1
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1
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4
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1
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8
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1
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1
2
1
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4
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1
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286507
Stafford
The enzymatic reduction of hyd ...
Beta vulgaris, Brassica oleracea, Daucus carota, Lactuca sativa, Petroselinum crispum, Pisum sativum, Raphanus sativus, Solanum lycopersicum, Spinacia oleracea
J. Biol. Chem.
207
621-629
1954
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9
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9
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18
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18
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9
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