Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hydroxypyruvate | product inhibition at a concentration above 4 mM, 99% inhibition of the reverse reaction at 0.5 mM | Methylorubrum extorquens | |
NADH | concentration above 0.5 mM | Methylorubrum extorquens | |
NADPH | concentration above 0.5 mM | Methylorubrum extorquens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
NADH | - |
Methylorubrum extorquens | |
0.06 | - |
NADPH | - |
Methylorubrum extorquens | |
0.1 | - |
Hydroxypyruvate | - |
Methylorubrum extorquens | |
0.12 | - |
Hydroxypyruvate | - |
Methylorubrum extorquens | |
1.5 | - |
glyoxylate | - |
Methylorubrum extorquens | |
2.6 | - |
glycerate | - |
Methylorubrum extorquens | |
20 | - |
glyoxylate | - |
Methylorubrum extorquens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
2 * 37000, SDS-PAGE | Methylorubrum extorquens |
71000 | - |
SDS-PAGE | Methylorubrum extorquens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylorubrum extorquens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methylorubrum extorquens |
Storage Stability | Organism |
---|---|
4°C, 50 mM Tris-HCl buffer, pH 7.5, containing 10% glycerol and 1 mM dithiothreitol, 2 months, at least 90% of inital activity | Methylorubrum extorquens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glycerate + NAD+ | - |
Methylorubrum extorquens | hydroxypyruvate + NADH + H+ | - |
r | |
glyoxylate + NADH | - |
Methylorubrum extorquens | glycolate + NAD+ | - |
ir | |
hydroxypyruvate + NADH | - |
Methylorubrum extorquens | D-glycerate + NAD+ | - |
r | |
hydroxypyruvate + NADPH + H+ | - |
Methylorubrum extorquens | D-glycerate + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37000, SDS-PAGE | Methylorubrum extorquens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 6.5 | reduction of hydroxypyruvate | Methylorubrum extorquens |
9 | 11 | glycerate oxidation | Methylorubrum extorquens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Methylorubrum extorquens | |
NADPH | - |
Methylorubrum extorquens |