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Literature summary for 1.1.1.29 extracted from

  • Izumi, Y.; Yoshida, T.; Kanzaki, H.; Toki, S.; Miyazaki, S.S.; Yamada, H.
    Purification and characterization of hydroxypyruvate reductase from a serine-producing methylotroph, Hyphomicrobium methylovorum GM2 (1990), Eur. J. Biochem., 190, 279-284.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
AgNO3
-
Hyphomicrobium methylovorum
CuSO4 slight Hyphomicrobium methylovorum
Dihydroxyfumarate
-
Hyphomicrobium methylovorum
HgCl2
-
Hyphomicrobium methylovorum
additional information not inhibitory: citrate, NADP+, EDTA, 1,10-phenanthroline, iodoacetate, iodoacetamide, N-ethylmaleimide, hydroxylamine, semicarbazide, NaN3, NaF, KCN, L-ascorbate, dithiothreitol, pyridoxal 5'-phosphate Hyphomicrobium methylovorum
p-chloromercuribenzoate
-
Hyphomicrobium methylovorum
phenylhydrazine
-
Hyphomicrobium methylovorum
ZnCl2 slight Hyphomicrobium methylovorum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.055
-
NADH
-
Hyphomicrobium methylovorum
0.175
-
Hydroxypyruvate
-
Hyphomicrobium methylovorum
10.8
-
glyoxylate
-
Hyphomicrobium methylovorum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
2 * 38000, SDS-PAGE Hyphomicrobium methylovorum
69000 70000 gel filtration Hyphomicrobium methylovorum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hydroxypyruvate + NADH Hyphomicrobium methylovorum
-
D-glycerate + NAD+
-
ir

Organism

Organism UniProt Comment Textmining
Hyphomicrobium methylovorum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Hyphomicrobium methylovorum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1086
-
-
Hyphomicrobium methylovorum

Storage Stability

Storage Stability Organism
-20°C, 10 mM potassium phosphate buffer, pH 7.0, 45% v/v glycerol, 1.0 M DTT, 18 months Hyphomicrobium methylovorum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glyoxylate + NADH
-
Hyphomicrobium methylovorum glycolate + NAD+
-
?
hydroxypyruvate + NADH
-
Hyphomicrobium methylovorum D-glycerate + NAD+
-
ir

Subunits

Subunits Comment Organism
dimer 2 * 38000, SDS-PAGE Hyphomicrobium methylovorum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
up to Hyphomicrobium methylovorum
30
-
30 min, 13.3% loss of activity Hyphomicrobium methylovorum
35
-
30 min, 30.8% loss of activity Hyphomicrobium methylovorum
40
-
30 min, 100% loss of activity Hyphomicrobium methylovorum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
Hyphomicrobium methylovorum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9
-
Hyphomicrobium methylovorum

Cofactor

Cofactor Comment Organism Structure
NADH specific for, not replaceable by NADPH Hyphomicrobium methylovorum