BRENDA - Enzyme Database show
show all sequences of 1.1.1.29

Purification and characterization of hydroxypyruvate reductase from a serine-producing methylotroph, Hyphomicrobium methylovorum GM2

Izumi, Y.; Yoshida, T.; Kanzaki, H.; Toki, S.; Miyazaki, S.S.; Yamada, H.; Eur. J. Biochem. 190, 279-284 (1990)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
AgNO3
-
Hyphomicrobium methylovorum
CuSO4
slight
Hyphomicrobium methylovorum
Dihydroxyfumarate
-
Hyphomicrobium methylovorum
HgCl2
-
Hyphomicrobium methylovorum
additional information
not inhibitory: citrate, NADP+, EDTA, 1,10-phenanthroline, iodoacetate, iodoacetamide, N-ethylmaleimide, hydroxylamine, semicarbazide, NaN3, NaF, KCN, L-ascorbate, dithiothreitol, pyridoxal 5'-phosphate
Hyphomicrobium methylovorum
p-chloromercuribenzoate
-
Hyphomicrobium methylovorum
phenylhydrazine
-
Hyphomicrobium methylovorum
ZnCl2
slight
Hyphomicrobium methylovorum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.055
-
NADH
-
Hyphomicrobium methylovorum
0.175
-
Hydroxypyruvate
-
Hyphomicrobium methylovorum
10.8
-
glyoxylate
-
Hyphomicrobium methylovorum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38000
-
2 * 38000, SDS-PAGE
Hyphomicrobium methylovorum
69000
70000
gel filtration
Hyphomicrobium methylovorum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
hydroxypyruvate + NADH
Hyphomicrobium methylovorum
-
D-glycerate + NAD+
-
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Hyphomicrobium methylovorum
-
-
-
Purification (Commentary)
Commentary
Organism
-
Hyphomicrobium methylovorum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1086
-
-
Hyphomicrobium methylovorum
Storage Stability
Storage Stability
Organism
-20C, 10 mM potassium phosphate buffer, pH 7.0, 45% v/v glycerol, 1.0 M DTT, 18 months
Hyphomicrobium methylovorum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glyoxylate + NADH
-
286506
Hyphomicrobium methylovorum
glycolate + NAD+
-
-
-
?
hydroxypyruvate + NADH
-
286506
Hyphomicrobium methylovorum
D-glycerate + NAD+
-
-
-
ir
Subunits
Subunits
Commentary
Organism
dimer
2 * 38000, SDS-PAGE
Hyphomicrobium methylovorum
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
25
-
up to
Hyphomicrobium methylovorum
30
-
30 min, 13.3% loss of activity
Hyphomicrobium methylovorum
35
-
30 min, 30.8% loss of activity
Hyphomicrobium methylovorum
40
-
30 min, 100% loss of activity
Hyphomicrobium methylovorum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
-
Hyphomicrobium methylovorum
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
9
-
Hyphomicrobium methylovorum
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
specific for, not replaceable by NADPH
Hyphomicrobium methylovorum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
specific for, not replaceable by NADPH
Hyphomicrobium methylovorum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
AgNO3
-
Hyphomicrobium methylovorum
CuSO4
slight
Hyphomicrobium methylovorum
Dihydroxyfumarate
-
Hyphomicrobium methylovorum
HgCl2
-
Hyphomicrobium methylovorum
additional information
not inhibitory: citrate, NADP+, EDTA, 1,10-phenanthroline, iodoacetate, iodoacetamide, N-ethylmaleimide, hydroxylamine, semicarbazide, NaN3, NaF, KCN, L-ascorbate, dithiothreitol, pyridoxal 5'-phosphate
Hyphomicrobium methylovorum
p-chloromercuribenzoate
-
Hyphomicrobium methylovorum
phenylhydrazine
-
Hyphomicrobium methylovorum
ZnCl2
slight
Hyphomicrobium methylovorum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.055
-
NADH
-
Hyphomicrobium methylovorum
0.175
-
Hydroxypyruvate
-
Hyphomicrobium methylovorum
10.8
-
glyoxylate
-
Hyphomicrobium methylovorum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38000
-
2 * 38000, SDS-PAGE
Hyphomicrobium methylovorum
69000
70000
gel filtration
Hyphomicrobium methylovorum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
hydroxypyruvate + NADH
Hyphomicrobium methylovorum
-
D-glycerate + NAD+
-
-
ir
Purification (Commentary) (protein specific)
Commentary
Organism
-
Hyphomicrobium methylovorum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1086
-
-
Hyphomicrobium methylovorum
Storage Stability (protein specific)
Storage Stability
Organism
-20C, 10 mM potassium phosphate buffer, pH 7.0, 45% v/v glycerol, 1.0 M DTT, 18 months
Hyphomicrobium methylovorum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glyoxylate + NADH
-
286506
Hyphomicrobium methylovorum
glycolate + NAD+
-
-
-
?
hydroxypyruvate + NADH
-
286506
Hyphomicrobium methylovorum
D-glycerate + NAD+
-
-
-
ir
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 38000, SDS-PAGE
Hyphomicrobium methylovorum
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
25
-
up to
Hyphomicrobium methylovorum
30
-
30 min, 13.3% loss of activity
Hyphomicrobium methylovorum
35
-
30 min, 30.8% loss of activity
Hyphomicrobium methylovorum
40
-
30 min, 100% loss of activity
Hyphomicrobium methylovorum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
-
Hyphomicrobium methylovorum
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
9
-
Hyphomicrobium methylovorum
Other publictions for EC 1.1.1.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737421
Subramanian
Glyoxalate reductase/hydroxypy ...
Homo sapiens
Am. J. Physiol. Gastrointest. Liver Physiol.
304
G1079-G1086
2013
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1
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3
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3
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1
1
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1
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1
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1
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3
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1
1
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1
1
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737736
Corpas
Inhibition of peroxisomal hydr ...
Arabidopsis thaliana
Biochim. Biophys. Acta
1830
4981-4989
2013
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1
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4
-
1
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1
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1
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1
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1
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1
1
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1
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1
1
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4
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1
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1
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1
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1
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1
1
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737745
Hoover
Identification of catalyticall ...
Arabidopsis thaliana
Biochim. Biophys. Acta
1834
2663-2671
2013
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1
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1
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739241
Pan
Glyoxylate reductase/hydroxypy ...
Homo sapiens
Pathobiology
80
155-162
2013
-
1
-
-
-
-
-
-
-
-
-
1
-
2
-
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6
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1
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1
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1
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1
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1
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6
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1
-
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1
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1
-
-
726102
Cousins
Peroxisomal hydroxypyruvate re ...
Arabidopsis thaliana
Photosyn. Res.
108
91-100
2011
-
-
-
-
-
-
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1
-
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1
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1
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1
1
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-
728436
Kern
Evolution of enzymes involved ...
Arabidopsis thaliana
Photosyn. Res.
109
103-114
2011
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-
-
-
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1
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1
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1
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1
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1
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1
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1
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1
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1
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-
-
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-
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-
286509
Julliard
Identification of hydroxypyruv ...
Petroselinum crispum
Protein Expr. Purif.
9
10-14
1997
-
-
-
-
-
-
-
2
1
-
2
1
-
1
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1
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1
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1
1
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2
1
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2
1
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1
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1
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1
1
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286510
Hagishita
Immunological characterization ...
Hyphomicrobium methylovorum
FEMS Microbiol. Lett.
142
49-52
1996
-
-
-
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1
1
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1
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1
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1
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1
1
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1
1
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1
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1
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1
1
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-
286505
Kleczkowski
Oxalate as a potent and select ...
Hordeum vulgare, Zea mays
Biochem. J.
276
125-127
1991
-
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1
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1
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2
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1
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1
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1
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1
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1
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2
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286508
Chistoserdova
Purification and characterizat ...
Methylobacterium extorquens
J. Bacteriol.
173
7228-7232
1991
-
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3
7
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2
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1
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1
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1
2
1
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2
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2
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2
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3
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7
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2
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1
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1
2
1
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2
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-
-
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-
286506
Izumi
Purification and characterizat ...
Hyphomicrobium methylovorum
Eur. J. Biochem.
190
279-284
1990
-
-
-
-
-
-
8
3
-
-
2
1
-
1
-
-
1
-
-
-
1
1
2
1
-
-
4
-
1
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1
1
-
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-
1
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8
-
3
-
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2
1
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1
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1
1
2
1
-
-
4
-
1
-
1
-
-
-
-
-
-
-
286507
Stafford
The enzymatic reduction of hyd ...
Beta vulgaris, Brassica oleracea, Daucus carota, Lactuca sativa, Petroselinum crispum, Pisum sativum, Raphanus sativus, Solanum lycopersicum, Spinacia oleracea
J. Biol. Chem.
207
621-629
1954
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9
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9
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9
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9
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18
-
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18
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9
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9
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9
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