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Literature summary for 1.1.1.287 extracted from
- Cloning, purification and characterization of an NAD-dependent D-arabitol dehydrogenase from acetic acid bacterium, Acetobacter suboxydans (2009), Protein J., 28, 263-272.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Gluconobacter oxydans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 10 mM, complete inhibition. Up to 80% renaturation by 100 mM EDTA | Gluconobacter oxydans |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.5 | - |
D-arabitol | pH 8.5, 25°C | Gluconobacter oxydans | |
| 18.5 | - |
xylitol | pH 8.5, 25°C | Gluconobacter oxydans | |
| 78.5 | - |
D-mannitol | pH 8.5, 25°C | Gluconobacter oxydans | |
| 127.3 | - |
D-sorbitol | pH 8.5, 25°C | Gluconobacter oxydans | |
| 133.6 | - |
glycerol | pH 8.5, 25°C | Gluconobacter oxydans | |
| 242.8 | - |
D-ribitol | pH 8.5, 25°C | Gluconobacter oxydans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | up to 200 mM, 2fold increase in activity | Gluconobacter oxydans | |
| Ca2+ | up to 200 mM, 2fold increase in activity | Gluconobacter oxydans | |
| additional information | no effect by addition of Mn2+, Ni2+, Mg2+ | Gluconobacter oxydans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28500 | - |
2 * 28500, SDS-PAGE and calculated, recombinant enzyme | Gluconobacter oxydans |
| 28500 | - |
4 * 28500, SDS-PAGE and calculated, native enzyme | Gluconobacter oxydans |
| 70000 | - |
gel filtration, recombinant enzyme | Gluconobacter oxydans |
| 140000 | - |
gel filtration, native enzyme | Gluconobacter oxydans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gluconobacter oxydans | Q308C1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme | Gluconobacter oxydans |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| inhibition by Cu2+ can partly be restored by EDTA | Gluconobacter oxydans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12.6 | - |
substrate D-xylitol, pH 8.5, 25°C | Gluconobacter oxydans |
| 16.4 | - |
substrate D-xylulose, pH 5.5, 25°C | Gluconobacter oxydans |
| 68.5 | - |
substrate D-arabitol, pH 8.5, 25°C | Gluconobacter oxydans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 100 mM Tris/HCl with 2 mM 2-mercaptoethanol, 50 days, 50% oxidative activity | Gluconobacter oxydans |
| 4°C, 100 mM Tris/HCl with 2 mM 2-mercaptoethanol, 80 days, 5% oxidative activity | Gluconobacter oxydans |
| 4°C, 100 mM Tris/HCl without 2 mM 2-mercaptoethanol, 20 days, complete loss of activity | Gluconobacter oxydans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-arabitol + NAD+ | - |
Gluconobacter oxydans | D-xylulose + NADH + H+ | - |
? | |
| D-arabitol + NADP+ | - |
Gluconobacter oxydans | D-xylulose + NADPH + H+ | - |
r | |
| D-mannitol + NAD+ | - |
Gluconobacter oxydans | D-fructose + NADH + H+ | - |
? | |
| D-ribitol + NAD+ | - |
Gluconobacter oxydans | D-ribulose + NADH + H+ | - |
? | |
| D-sorbitol + NAD+ | - |
Gluconobacter oxydans | sorbose + NADH + H+ | - |
? | |
| D-xylulose + NAD+ | - |
Gluconobacter oxydans | D-arabitol + NADH + H+ | - |
r | |
| glycerol + NAD+ | - |
Gluconobacter oxydans | dihydroxyacetone + NADH + H+ | - |
? | |
| xylitol + NAD+ | - |
Gluconobacter oxydans | D-xylulose + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 28500, SDS-PAGE and calculated, recombinant enzyme | Gluconobacter oxydans |
| tetramer | 4 * 28500, SDS-PAGE and calculated, native enzyme | Gluconobacter oxydans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Gluconobacter oxydans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
2% of maximum activity | Gluconobacter oxydans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 13.4 | - |
D-ribitol | pH 8.5, 25°C | Gluconobacter oxydans | |
| 16.3 | - |
glycerol | pH 8.5, 25°C | Gluconobacter oxydans | |
| 19.7 | - |
D-sorbitol | pH 8.5, 25°C | Gluconobacter oxydans | |
| 35.3 | - |
D-mannitol | pH 8.5, 25°C | Gluconobacter oxydans | |
| 177.2 | - |
xylitol | pH 8.5, 25°C | Gluconobacter oxydans | |
| 876 | - |
D-arabitol | pH 8.5, 25°C | Gluconobacter oxydans | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
ketone reduction | Gluconobacter oxydans |
| 8.5 | - |
polyol oxidation | Gluconobacter oxydans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | NADH is preferred over NADPH | Gluconobacter oxydans | |
| NADPH | NADH is preferred over NADPH | Gluconobacter oxydans | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Gluconobacter oxydans | isoelectric focusing | - |
5.9 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Gluconobacter oxydans | induced by growth on D-arabinitol | up |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
D-ribitol | pH 8.5, 25°C | Gluconobacter oxydans | |
| 0.12 | - |
glycerol | pH 8.5, 25°C | Gluconobacter oxydans | |
| 0.15 | - |
D-sorbitol | pH 8.5, 25°C | Gluconobacter oxydans | |
| 0.45 | - |
D-mannitol | pH 8.5, 25°C | Gluconobacter oxydans | |
| 9.58 | - |
xylitol | pH 8.5, 25°C | Gluconobacter oxydans | |
| 194.7 | - |
D-arabitol | pH 8.5, 25°C | Gluconobacter oxydans | |
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