Cloned (Comment) | Organism |
---|---|
gene ARD1, DNA and amino acid sequence determination and analysis, expression in Saccharomyces cerevisiae strain 23344c, overexpression in Escherichia coli strain BL21(DE3) as N-terminally His6-tagged protein | Uromyces viciae-fabae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Uromyces viciae-fabae | |
0.07 | - |
NADP+ | pH 9.0, with D-mannitol | Uromyces viciae-fabae | |
0.08 | - |
NADP+ | pH 9.0, with D-arabitinol | Uromyces viciae-fabae | |
0.16 | - |
NADPH | pH 6.0, with D-fructose | Uromyces viciae-fabae | |
0.2 | - |
NADPH | pH 6.0, with D-ribulose | Uromyces viciae-fabae | |
0.26 | - |
NADPH | pH 6.0, with D-xylulose | Uromyces viciae-fabae | |
7.8 | - |
D-xylulose | pH 6.0 | Uromyces viciae-fabae | |
53.9 | - |
D-ribulose | pH 6.0 | Uromyces viciae-fabae | |
160 | - |
D-fructose | pH 6.0 | Uromyces viciae-fabae | |
425 | - |
D-mannitol | pH 9.0 | Uromyces viciae-fabae | |
834 | - |
D-arabitinol | pH 9.0 | Uromyces viciae-fabae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Uromyces viciae-fabae | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Uromyces viciae-fabae | the enzyme activity is highly increased during infection processes and pathogenesis, development after infection, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Uromyces viciae-fabae | Q4R0J7 | cultivated on artificial surfaces in in vitro infection structures | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Uromyces viciae-fabae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
haustorium | exclusively, in the lumen | Uromyces viciae-fabae | - |
additional information | cultivated on artificial surfaces in in vitro infection structures | Uromyces viciae-fabae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabitinol + NADP+ | - |
Uromyces viciae-fabae | D-xylulose + NADPH + H+ | - |
r | |
D-arabitinol + NADP+ | - |
Uromyces viciae-fabae | D-ribulose + NADPH + H+ | - |
r | |
D-mannitol + NADP+ | - |
Uromyces viciae-fabae | D-fructose + NADPH + H+ | - |
r | |
additional information | the enzyme activity is highly increased during infection processes and pathogenesis, development after infection, overview | Uromyces viciae-fabae | ? | - |
? | |
additional information | the enzyme shows high substrate specificity, the reverse reaction is preferred | Uromyces viciae-fabae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ARD1p | - |
Uromyces viciae-fabae |
D-arabitol dehydrogenase 1 | - |
Uromyces viciae-fabae |
NADP(+)-dependent D-arabitol dehydrogenase | - |
Uromyces viciae-fabae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at, reverse reaction | Uromyces viciae-fabae |
9 | - |
assay at, forward reaction | Uromyces viciae-fabae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | dependent on, absolutely specific for, forward reaction | Uromyces viciae-fabae | |
NADPH | dependent on, absolutely specific for, reverse reaction | Uromyces viciae-fabae |