General Stability | Organism |
---|---|
inactivated by freezing and thawing | [Candida] boidinii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 1 mM, complete inhibition | [Candida] boidinii | |
CN- | 1 mM, 42% inhibition | [Candida] boidinii | |
Cu2+ | 1 mM, complete inhibition | [Candida] boidinii | |
Hg2+ | 1 mM, complete inhibition | [Candida] boidinii | |
iodoacetate | 1 mM, 15% inhibition | [Candida] boidinii | |
PCMB | 1 mM, complete inhibition | [Candida] boidinii | |
Zn2+ | 1 mM, 50% inhibition | [Candida] boidinii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
NAD+ | - |
[Candida] boidinii | |
0.13 | - |
glutathione | - |
[Candida] boidinii | |
0.25 | - |
formaldehyde | - |
[Candida] boidinii | |
1.2 | - |
methylglyoxal | - |
[Candida] boidinii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | [Candida] boidinii |
80000 | - |
equilibrium sedimentation | [Candida] boidinii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + glutathione + NAD+ | [Candida] boidinii | enzyme is found only in methanol-grown cells and is absent in cells grown on ethanol or glucose as carbon source | S-formylglutathione + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Candida] boidinii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
[Candida] boidinii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
47.9 | - |
- |
[Candida] boidinii |
Storage Stability | Organism |
---|---|
50% glycerol, stable for a long time | [Candida] boidinii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + glutathione + NAD+ | - |
[Candida] boidinii | S-formylglutathione + NADH + H+ | - |
? | |
formaldehyde + glutathione + NAD+ | enzyme is found only in methanol-grown cells and is absent in cells grown on ethanol or glucose as carbon source | [Candida] boidinii | S-formylglutathione + NADH + H+ | - |
? | |
methylglyoxal + glutathione + NAD+ | - |
[Candida] boidinii | S-pyruvylglutathione + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE | [Candida] boidinii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | - |
[Candida] boidinii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
[Candida] boidinii |