Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 1 mM, 30% inhibition | Komagataella pastoris | |
2,2'-dipyridyl | 1 mM, 20% inhibition | Komagataella pastoris | |
Cd2+ | 1 mM, complete inhibition | Komagataella pastoris | |
Cu2+ | - |
Komagataella pastoris | |
Hg2+ | 1 mM, complete inhibition | Komagataella pastoris | |
imidazole | 1 mM, 32% inhibition | Komagataella pastoris | |
iodoacetamide | 1 mM, 20% inhibition | Komagataella pastoris | |
NEM | 1 mM, 35% inhibition | Komagataella pastoris | |
PCMB | 1 mM, complete inhibition | Komagataella pastoris | |
Zn2+ | 1 mM, 40% inhibition | Komagataella pastoris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
NAD+ | - |
Komagataella pastoris | |
0.16 | - |
reduced glutathione | - |
Komagataella pastoris | |
0.25 | - |
NADH | - |
Komagataella pastoris | |
0.31 | - |
formaldehyde | - |
Komagataella pastoris | |
0.6 | - |
S-formylglutathione | - |
Komagataella pastoris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
2 * 41000, SDS-PAGE | Komagataella pastoris |
84000 | - |
gel filtration | Komagataella pastoris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Komagataella pastoris | - |
NRRL-Y-11328 | - |
Komagataella pastoris NRRL-Y-11328 | - |
NRRL-Y-11328 | - |
Purification (Comment) | Organism |
---|---|
- |
Komagataella pastoris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2090 | - |
- |
Komagataella pastoris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + glutathione + NAD+ | - |
Komagataella pastoris | S-formylglutathione + NADH + H+ | - |
r | |
formaldehyde + glutathione + NAD+ | - |
Komagataella pastoris NRRL-Y-11328 | S-formylglutathione + NADH + H+ | - |
r | |
methylglyoxal + glutathione + NAD+ | - |
Komagataella pastoris | S-pyruvylglutathione + NADH | - |
? | |
methylglyoxal + glutathione + NAD+ | - |
Komagataella pastoris NRRL-Y-11328 | S-pyruvylglutathione + NADH | - |
? | |
S-formylglutathione + NADH | - |
Komagataella pastoris | formaldehyde + glutathione + NAD+ | - |
r | |
S-formylglutathione + NADH | - |
Komagataella pastoris NRRL-Y-11328 | formaldehyde + glutathione + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 41000, SDS-PAGE | Komagataella pastoris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
oxidation of formaldehyde | Komagataella pastoris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | pH 7.0: about 55% of maximal activity, pH 9.0: about 90% of maximal activity | Komagataella pastoris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Komagataella pastoris | |
NADH | - |
Komagataella pastoris | |
NADP+ | no activity with NADP+ | Komagataella pastoris |