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Literature summary for 1.1.1.284 extracted from

  • Patel, R.N.; Hou, C.T.; Derelanko, P.
    Microbial oxidation of methanol: purification and properties of formaldehyde dehydrogenase from a Pichia sp. NRRL-Y-11328 (1983), Arch. Biochem. Biophys., 221, 135-142.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM, 30% inhibition Komagataella pastoris
2,2'-dipyridyl 1 mM, 20% inhibition Komagataella pastoris
Cd2+ 1 mM, complete inhibition Komagataella pastoris
Cu2+
-
Komagataella pastoris
Hg2+ 1 mM, complete inhibition Komagataella pastoris
imidazole 1 mM, 32% inhibition Komagataella pastoris
iodoacetamide 1 mM, 20% inhibition Komagataella pastoris
NEM 1 mM, 35% inhibition Komagataella pastoris
PCMB 1 mM, complete inhibition Komagataella pastoris
Zn2+ 1 mM, 40% inhibition Komagataella pastoris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
NAD+
-
Komagataella pastoris
0.16
-
reduced glutathione
-
Komagataella pastoris
0.25
-
NADH
-
Komagataella pastoris
0.31
-
formaldehyde
-
Komagataella pastoris
0.6
-
S-formylglutathione
-
Komagataella pastoris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
2 * 41000, SDS-PAGE Komagataella pastoris
84000
-
gel filtration Komagataella pastoris

Organism

Organism UniProt Comment Textmining
Komagataella pastoris
-
NRRL-Y-11328
-
Komagataella pastoris NRRL-Y-11328
-
NRRL-Y-11328
-

Purification (Commentary)

Purification (Comment) Organism
-
Komagataella pastoris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2090
-
-
Komagataella pastoris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formaldehyde + glutathione + NAD+
-
Komagataella pastoris S-formylglutathione + NADH + H+
-
r
formaldehyde + glutathione + NAD+
-
Komagataella pastoris NRRL-Y-11328 S-formylglutathione + NADH + H+
-
r
methylglyoxal + glutathione + NAD+
-
Komagataella pastoris S-pyruvylglutathione + NADH
-
?
methylglyoxal + glutathione + NAD+
-
Komagataella pastoris NRRL-Y-11328 S-pyruvylglutathione + NADH
-
?
S-formylglutathione + NADH
-
Komagataella pastoris formaldehyde + glutathione + NAD+
-
r
S-formylglutathione + NADH
-
Komagataella pastoris NRRL-Y-11328 formaldehyde + glutathione + NAD+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 41000, SDS-PAGE Komagataella pastoris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
oxidation of formaldehyde Komagataella pastoris

pH Range

pH Minimum pH Maximum Comment Organism
7 9 pH 7.0: about 55% of maximal activity, pH 9.0: about 90% of maximal activity Komagataella pastoris

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Komagataella pastoris
NADH
-
Komagataella pastoris
NADP+ no activity with NADP+ Komagataella pastoris