Activating Compound | Comment | Organism | Structure |
---|---|---|---|
iodoacetate | activation of enzyme MGR I, inhibition of enzyme MGR II | Aspergillus niger | |
N-ethylmaleimide | activation of enzyme MGR I, inhibition of enzyme MGR II | Aspergillus niger | |
p-chloromercuribenzoate | activation of enzyme MGR I, inhibition of enzyme MGR II | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Aspergillus niger | |
Ca2+ | enzymes MGR I (slightly), MGR II | Aspergillus niger | |
Co2+ | activates enzyme MGR I, slightly inhibits enzyme MGR II | Aspergillus niger | |
Cu2+ | enzymes MGR I and MGR II | Aspergillus niger | |
dithiothreitol | - |
Aspergillus niger | |
EDTA | - |
Aspergillus niger | |
Glyoxal | in absence of NADPH | Aspergillus niger | |
iodoacetate | activation of enzyme MGR I, inhibition of enzyme MGR II | Aspergillus niger | |
methylglyoxal | in absence of NADPH | Aspergillus niger | |
Mg2+ | enzymes MGR I and MGR II | Aspergillus niger | |
Mn2+ | enzymes MGR I, MGR II | Aspergillus niger | |
N-ethylmaleimide | activation of enzyme MGR I, inhibition of enzyme MGR II | Aspergillus niger | |
NADP+ | - |
Aspergillus niger | |
Ni2+ | enzyme MGR I | Aspergillus niger | |
p-chloromercuribenzoate | activation of enzyme MGR I, inhibition of enzyme MGR II | Aspergillus niger | |
Phenylglyoxal | in absence of NADPH | Aspergillus niger | |
Zn2+ | enzyme MGR I | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0068 | - |
NADPH | enzyme MGR II | Aspergillus niger | |
0.054 | - |
NADPH | enzyme MGR I | Aspergillus niger | |
0.7 | - |
methylglyoxal | - |
Aspergillus niger | |
1.43 | - |
methylglyoxal | enzyme MGR I | Aspergillus niger | |
4.35 | - |
Phenylglyoxal | enzyme MGR II | Aspergillus niger | |
10 | - |
Glyoxal | enzyme MGR II | Aspergillus niger | |
15.4 | - |
methylglyoxal | enzyme MGR I | Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates enzyme MGR I, slightly inhibits enzyme MGR II | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
1 * 36000, enzyme MGR I, 1 * 38000, enzyme MGR II, similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+, SDS-PAGE | Aspergillus niger |
37000 | - |
enzyme MGR I, similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+, gel filtration | Aspergillus niger |
38000 | - |
enzyme MGR II, similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+, gel filtration | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylglyoxal + NADPH | Aspergillus niger | similar enzyme with NADPH requirement | lactaldehyde + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
2 methylglyoxylate reductases: MGR I and MGR II, similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+ | - |
Purification (Comment) | Organism |
---|---|
two methylglyoxylate reductases: MGR I and MGR II, similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+ | Aspergillus niger |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-lactaldehyde + NADP+ = 2-oxopropanal + NADPH + H+ | similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+ | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.83 | - |
enzyme MGR I | Aspergillus niger |
7.77 | - |
enzyme MGR II | Aspergillus niger |
Storage Stability | Organism |
---|---|
4°C | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NADPH + H+ | enzyme MGR II | Aspergillus niger | ethanol + NADP+ | - |
? | |
glyoxal + NADPH | enzymes MGR I and MGR II | Aspergillus niger | glycolaldehyde + NADP+ | - |
? | |
methylglyoxal + NADPH | similar enzyme with NADPH requirement | Aspergillus niger | lactaldehyde + NADP+ | - |
? | |
additional information | enzyme MGR I: specific for 2-oxoaldehydes (glyoxal phenylglyoxal), enzyme MGR II: active towards 2-oxoaldehydes (glyoxal, methylglyoxal, phenylglyoxal), 4,5-dioxovalerate and some aldehydes (propionaldehyde and acetaldehyde) | Aspergillus niger | ? | - |
? | |
phenylglyoxal + NADPH | enzymes MGR I and MGR II | Aspergillus niger | hydroxyphenylacetaldehyde + NADP+ | - |
? | |
propionaldehyde + NADPH | enzyme MGR II | Aspergillus niger | propanol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36000, enzyme MGR I, 1 * 38000, enzyme MGR II, similar enzyme with NADPH-requirement for methylglyoxal reduction, that is inactive with NAD+, NADH and NADP+, SDS-PAGE | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
enzyme MGR I | Aspergillus niger |
9 | - |
enzyme MGR II | Aspergillus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | NADPH required | Aspergillus niger |