Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.28 extracted from

  • Jun, C.; Sa, Y.; Gu, S.; Joo, J.; Kim, S.; Kim, K.; Kim, Y.
    Discovery and characterization of a thermostable D-lactate dehydrogenase from Lactobacillus jensenii through genome mining (2013), Process Biochem., 48, 109-117.
No PubMed abstract available
Search for more literature for 1.1.1.28

Cloned(Commentary)

Cloned (Comment) Organism
D-LDH isozymes D-LDH1, D-LDH2, and D-LDH3, sequence comparisons and phylogenetic analysis Lactobacillus jensenii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 NADH isozyme D-LDH3, pH 8.0, 25°C Lactobacillus jensenii
0.41
-
 NAD+ isozyme D-LDH3, pH 8.0, 25°C Lactobacillus jensenii
0.7
-
 NADH isozyme D-LDH1, pH 8.0, 25°C Lactobacillus jensenii
2.58
-
 NAD+ isozyme D-LDH2, pH 8.0, 25°C Lactobacillus jensenii
2.75
-
 NAD+ isozyme D-LDH1, pH 8.0, 25°C Lactobacillus jensenii
2.83
-
 NADH isozyme D-LDH2, pH 8.0, 25°C Lactobacillus jensenii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-lactate + NAD+ Lactobacillus jensenii
-
 pyruvate + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Lactobacillus jensenii
-
 three isozymes, D-LDH1, D-LDH2, and D-LDH3
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7
-
 isozyme D-LDH1, pH 8.0, 25°C, substrate D-phenylpyruvate Lactobacillus jensenii
9
-
 isozyme D-LDH2, pH 8.0, 25°C, substrate D-phenylpyruvate Lactobacillus jensenii
18
-
 isozyme D-LDH3, pH 8.0, 25°C, substrate oxaloacetic acid Lactobacillus jensenii
28
-
 isozyme D-LDH3, pH 8.0, 25°C, substrate D-phenylpyruvate Lactobacillus jensenii
41
-
 isozyme D-LDH2, pH 8.0, 25°C, substrate 2-oxoobutyric acid Lactobacillus jensenii
41.91
-
 isozyme D-LDH3, pH 8.0, 25°C, substrate D-lactate Lactobacillus jensenii
68
-
 isozyme D-LDH1, pH 8.0, 25°C, substrate 2-oxoobutyric acid Lactobacillus jensenii
88.71
-
 isozyme D-LDH2, pH 8.0, 25°C, substrate D-lactate Lactobacillus jensenii
96.88
-
 isozyme D-LDH1, pH 8.0, 25°C, substrate D-lactate Lactobacillus jensenii
189
-
 isozyme D-LDH3, pH 8.0, 25°C, substrate 2-oxoobutyric acid Lactobacillus jensenii
348
-
 isozyme D-LDH3, pH 8.0, 25°C, substrate pyruvate Lactobacillus jensenii
2877
-
 isozyme D-LDH2, pH 8.0, 25°C, substrate oxaloacetic acid Lactobacillus jensenii
2997
-
 isozyme D-LDH2, pH 8.0, 25°C, substrate pyruvate Lactobacillus jensenii
3151
-
 isozyme D-LDH1, pH 8.0, 25°C, substrate oxaloacetic acid Lactobacillus jensenii
3838
-
 isozyme D-LDH1, pH 8.0, 25°C, substrate pyruvate Lactobacillus jensenii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-lactate + NAD+
-
 Lactobacillus jensenii pyruvate + NADH + H+
-
 r
(R)-lactate + NAD+ no activity with L-lactate Lactobacillus jensenii pyruvate + NADH + H+
-
 r
2-oxobutyric acid + NADH + H+
-
 Lactobacillus jensenii (R)-2-hydroxybutyric acid + NAD+
-
 ir
additional information substrate specificities of the D-LDH isozymes, overview. (R)-2-Hydroxybutyric acid, D-(+)-malic acid, and D-(+)-3-phenyllactic acid are no substrates Lactobacillus jensenii ?
-
 ?
oxaloacetic acid + NADH + H+
-
 Lactobacillus jensenii (R)-malate + NAD+
-
 ir
phenylpyruvate + NADH + H+
-
 Lactobacillus jensenii D-phenyllactate + NAD+
-
 ir

Synonyms

Synonyms Comment Organism
D-LDH
-
 Lactobacillus jensenii
D-LDH1
-
 Lactobacillus jensenii
D-LDH2
-
 Lactobacillus jensenii
D-LDH3
-
 Lactobacillus jensenii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 isozymes D-LDH1 and D-LDH2 Lactobacillus jensenii
40
-
 isozyme D-LDH3 Lactobacillus jensenii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 isozyme D-LDH3, activity range, profile, overview Lactobacillus jensenii
10 55 isozymes D-LDH1 and D-LDH2, activity range, profile, overview Lactobacillus jensenii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 55 thermal stability of the D-LDH isozymes, pH 7.0, with sodium pyruvate as a substrate, profiles, overview Lactobacillus jensenii
40
-
 all isozymes, stable up to Lactobacillus jensenii
50
-
 isozyme D-LDH1, inactivation Lactobacillus jensenii
55
-
 isozymes D-LDH2 and D-LDH3, inactivation Lactobacillus jensenii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.64
-
 NAD+ isozyme D-LDH3, pH 8.0, 25°C Lactobacillus jensenii
3.65
-
 NAD+ isozyme D-LDH2, pH 8.0, 25°C Lactobacillus jensenii
4.42
-
 NAD+ isozyme D-LDH1, pH 8.0, 25°C Lactobacillus jensenii
8.28
-
 NADH isozyme D-LDH3, pH 8.0, 25°C Lactobacillus jensenii
144.4
-
 NADH isozyme D-LDH1, pH 8.0, 25°C Lactobacillus jensenii
516.4
-
 NADH isozyme D-LDH2, pH 8.0, 25°C Lactobacillus jensenii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 isozymes D-LDH1 and D-LDH2 Lactobacillus jensenii
10
-
 isozyme D-LDH3 Lactobacillus jensenii

pH Range

pH Minimum pH Maximum Comment Organism
6 11 isozyme D-LDH3, activity range, profile, overview Lactobacillus jensenii
7.5 11 isozymes D-LDH1 and D-LDH2, activity range, profile, overview Lactobacillus jensenii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Lactobacillus jensenii
NADH
-
 Lactobacillus jensenii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.41
-
 NAD+ isozyme D-LDH2, pH 8.0, 25°C Lactobacillus jensenii
1.56
-
 NAD+ isozyme D-LDH3, pH 8.0, 25°C Lactobacillus jensenii
1.61
-
 NAD+ isozyme D-LDH1, pH 8.0, 25°C Lactobacillus jensenii
182.6
-
 NADH isozyme D-LDH2, pH 8.0, 25°C Lactobacillus jensenii
206.3
-
 NADH isozyme D-LDH1, pH 8.0, 25°C Lactobacillus jensenii
232.6
-
 NADH isozyme D-LDH3, pH 8.0, 25°C Lactobacillus jensenii