BRENDA - Enzyme Database show
show all sequences of 1.1.1.28

Recognition site for the side chain of 2-ketoacid substrate in D-lactate dehydrogenase

Ishikura, Y.; Tsuzuki, S.; Takahashi, O.; Tokuda, C.; Nakanishi, R.; Shinoda, T.; Taguchi, H.; J. Biochem. 138, 741-749 (2005)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
F299G
mutation reduces enzyme activity with less marked change in substrate preference
Lactobacillus pentosus
F299S
mutation reduces enzyme activity with less marked change in substrate preference
Lactobacillus pentosus
Y52A
mutation induces high activity and preference for large aliphatic 2-ketoacids and phenylpyruvate
Lactobacillus pentosus
Y52D
mutation severely reduces enzyme activity
Lactobacillus pentosus
Y52R
mutation severely reduces enzyme activity, preference for large aliphatic 2-ketoacids and phenylpyruvate
Lactobacillus pentosus
Y52R/F299G
mutation abolishes activity with pyruvate, 2-ketobutyrate, 2-ketovalerate, 2-ketoisovalerate, 2-ketoisocaproate and oxaloacetate, weak activity with 2-ketocaproate, 2-ketoglutarate and phenylpyruvate
Lactobacillus pentosus
Y52T
mutation severely reduces enzyme activity
Lactobacillus pentosus
Y52T/F299S
mutation abolishes activity with pyruvate, 2-ketobutyrate, 2-ketovalerate, 2-ketoisovalerate, 2-ketoisocaproate, 2-ketoglutarate and oxaloacetate, weak activity with hydroxypyruvate and phenylpyruvate
Lactobacillus pentosus
Y52V
mutation induces high activity and preference for large aliphatic 2-ketoacids and phenylpyruvate
Lactobacillus pentosus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
0.06
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
0.12
-
pyruvate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
0.124
-
2-ketoisocaproate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
0.14
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
0.15
-
2-Ketovalerate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
0.28
-
Hydroxypyruvate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
0.38
-
2-Ketobutyrate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
0.7
-
2-ketoisocaproate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
0.8
-
2-Ketovalerate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
0.8
-
phenylpyruvate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
1.18
-
2-ketocaproate
30°C, pH 5.5, mutant enzyme Y52R
Lactobacillus pentosus
1.8
-
pyruvate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
2.1
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme Y52R/F299G
Lactobacillus pentosus
2.1
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme Y52R
Lactobacillus pentosus
2.2
-
oxaloacetate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
3
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
3.5
-
2-Ketobutyrate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
3.8
-
oxaloacetate
30°C, pH 5.5, mutant enzyme Y52R
Lactobacillus pentosus
5.5
-
2-ketoisovalerate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
6.9
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme Y52R/F299G
Lactobacillus pentosus
7.2
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme F299S
Lactobacillus pentosus
8.1
-
2-Ketobutyrate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
8.7
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
8.7
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme Y52T/D299S
Lactobacillus pentosus
11.7
-
2-Ketovalerate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
12.1
-
2-ketoisocaproate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
12.6
-
pyruvate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
13.1
-
pyruvate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
13.3
-
2-ketocaproate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
14.1
-
2-ketocaproate
30°C, pH 5.5, mutant enzyme Y52R/F299G
Lactobacillus pentosus
15.5
-
2-Ketoglutarate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
17
-
2-Ketovalerate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
17
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
18.6
-
2-ketoisovalerate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
18.6
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme F299S
Lactobacillus pentosus
20.2
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme Y52R
Lactobacillus pentosus
20.8
-
2-Ketobutyrate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
22
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme F299G
Lactobacillus pentosus
31
-
2-ketoisocaproate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
31
-
pyruvate
30°C, pH 5.5, mutant enzyme F299G
Lactobacillus pentosus
43.1
-
oxaloacetate
30°C, pH 5.5, mutant enzyme F299S
Lactobacillus pentosus
51
-
2-ketocaproate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
53
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme F299G
Lactobacillus pentosus
102
-
2-ketocaproate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
189
-
2-ketocaproate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lactobacillus pentosus
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-ketocaproate + NADH
-
669152
Lactobacillus pentosus
2-hydroxycaproate + NAD+
-
-
-
?
2-ketoisocaproate + NADH
-
669152
Lactobacillus pentosus
2-hydroxyisocaproate + NAD+
-
-
-
?
2-ketoisovalerate + NADH
-
669152
Lactobacillus pentosus
2-hydroxyisovalerate + NAD+
-
-
-
?
2-ketovalerate + NADH
-
669152
Lactobacillus pentosus
2-hydroxyvalerate + NAD+
-
-
-
?
2-oxobutyrate + NADH + H+
-
669152
Lactobacillus pentosus
2-hydroxybutyrate + NAD+
-
-
-
?
2-oxoglutarate + NADH + H+
-
669152
Lactobacillus pentosus
2-hydroxypentanedioate + NAD+
-
-
-
?
hydroxypyruvate + NADH
-
669152
Lactobacillus pentosus
glycerate + NAD+
-
-
-
?
oxaloacetate + NADH
-
669152
Lactobacillus pentosus
malate + NAD+
-
-
-
?
phenylpyruvate + NADH
-
669152
Lactobacillus pentosus
phenyllactate + NAD+
-
-
-
?
pyruvate + NADH + H+
-
669152
Lactobacillus pentosus
(R)-lactate + NAD+
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.056
-
2-ketocaproate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
0.3
-
2-ketocaproate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
0.31
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme Y52R
Lactobacillus pentosus
0.36
-
2-ketocaproate
30°C, pH 5.5, mutant enzyme Y52R/F299G
Lactobacillus pentosus
0.48
-
oxaloacetate
30°C, pH 5.5, mutant enzyme Y52R
Lactobacillus pentosus
0.51
-
2-Ketoglutarate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
0.7
-
2-ketocaproate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
2.1
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme F299S
Lactobacillus pentosus
2.8
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme Y52R/F299G
Lactobacillus pentosus
3.4
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme F299G
Lactobacillus pentosus
5.2
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme Y52T/D299S
Lactobacillus pentosus
5.7
-
2-Ketovalerate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
6.5
-
oxaloacetate
30°C, pH 5.5, mutant enzyme F299S
Lactobacillus pentosus
8.1
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme F299G
Lactobacillus pentosus
11.1
-
2-ketoisocaproate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
11.7
-
2-ketoisovalerate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
13.8
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme Y52R
Lactobacillus pentosus
15.8
-
2-ketoisocaproate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
15.9
-
2-ketocaproate
30°C, pH 5.5, mutant enzyme Y52R
Lactobacillus pentosus
17
-
2-ketoisocaproate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
19
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme Y52R/F299G
Lactobacillus pentosus
21
-
2-ketocaproate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
22
-
pyruvate
30°C, pH 5.5, mutant enzyme F299G
Lactobacillus pentosus
25.5
-
pyruvate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
27
-
2-ketoisovalerate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
35.8
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme F299S
Lactobacillus pentosus
37
-
pyruvate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
40
-
phenylpyruvate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
41
-
2-Ketobutyrate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
41.5
-
2-Ketobutyrate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
50
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
55.7
-
2-Ketovalerate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
65.7
-
2-ketoisocaproate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
102
-
2-Ketovalerate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
104
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
118
-
2-Ketobutyrate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
171
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
175
-
2-Ketobutyrate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
213
-
pyruvate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
232
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
257
-
Hydroxypyruvate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
280
-
oxaloacetate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
321
-
pyruvate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
334
-
2-Ketovalerate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
407
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
778
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Lactobacillus pentosus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Lactobacillus pentosus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
F299G
mutation reduces enzyme activity with less marked change in substrate preference
Lactobacillus pentosus
F299S
mutation reduces enzyme activity with less marked change in substrate preference
Lactobacillus pentosus
Y52A
mutation induces high activity and preference for large aliphatic 2-ketoacids and phenylpyruvate
Lactobacillus pentosus
Y52D
mutation severely reduces enzyme activity
Lactobacillus pentosus
Y52R
mutation severely reduces enzyme activity, preference for large aliphatic 2-ketoacids and phenylpyruvate
Lactobacillus pentosus
Y52R/F299G
mutation abolishes activity with pyruvate, 2-ketobutyrate, 2-ketovalerate, 2-ketoisovalerate, 2-ketoisocaproate and oxaloacetate, weak activity with 2-ketocaproate, 2-ketoglutarate and phenylpyruvate
Lactobacillus pentosus
Y52T
mutation severely reduces enzyme activity
Lactobacillus pentosus
Y52T/F299S
mutation abolishes activity with pyruvate, 2-ketobutyrate, 2-ketovalerate, 2-ketoisovalerate, 2-ketoisocaproate, 2-ketoglutarate and oxaloacetate, weak activity with hydroxypyruvate and phenylpyruvate
Lactobacillus pentosus
Y52V
mutation induces high activity and preference for large aliphatic 2-ketoacids and phenylpyruvate
Lactobacillus pentosus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
0.06
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
0.12
-
pyruvate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
0.124
-
2-ketoisocaproate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
0.14
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
0.15
-
2-Ketovalerate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
0.28
-
Hydroxypyruvate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
0.38
-
2-Ketobutyrate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
0.7
-
2-ketoisocaproate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
0.8
-
2-Ketovalerate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
0.8
-
phenylpyruvate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
1.18
-
2-ketocaproate
30°C, pH 5.5, mutant enzyme Y52R
Lactobacillus pentosus
1.8
-
pyruvate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
2.1
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme Y52R/F299G
Lactobacillus pentosus
2.1
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme Y52R
Lactobacillus pentosus
2.2
-
oxaloacetate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
3
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
3.5
-
2-Ketobutyrate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
3.8
-
oxaloacetate
30°C, pH 5.5, mutant enzyme Y52R
Lactobacillus pentosus
5.5
-
2-ketoisovalerate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
6.9
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme Y52R/F299G
Lactobacillus pentosus
7.2
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme F299S
Lactobacillus pentosus
8.1
-
2-Ketobutyrate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
8.7
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
8.7
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme Y52T/D299S
Lactobacillus pentosus
11.7
-
2-Ketovalerate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
12.1
-
2-ketoisocaproate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
12.6
-
pyruvate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
13.1
-
pyruvate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
13.3
-
2-ketocaproate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
14.1
-
2-ketocaproate
30°C, pH 5.5, mutant enzyme Y52R/F299G
Lactobacillus pentosus
15.5
-
2-Ketoglutarate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
17
-
2-Ketovalerate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
17
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
18.6
-
2-ketoisovalerate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
18.6
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme F299S
Lactobacillus pentosus
20.2
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme Y52R
Lactobacillus pentosus
20.8
-
2-Ketobutyrate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
22
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme F299G
Lactobacillus pentosus
31
-
2-ketoisocaproate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
31
-
pyruvate
30°C, pH 5.5, mutant enzyme F299G
Lactobacillus pentosus
43.1
-
oxaloacetate
30°C, pH 5.5, mutant enzyme F299S
Lactobacillus pentosus
51
-
2-ketocaproate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
53
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme F299G
Lactobacillus pentosus
102
-
2-ketocaproate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
189
-
2-ketocaproate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-ketocaproate + NADH
-
669152
Lactobacillus pentosus
2-hydroxycaproate + NAD+
-
-
-
?
2-ketoisocaproate + NADH
-
669152
Lactobacillus pentosus
2-hydroxyisocaproate + NAD+
-
-
-
?
2-ketoisovalerate + NADH
-
669152
Lactobacillus pentosus
2-hydroxyisovalerate + NAD+
-
-
-
?
2-ketovalerate + NADH
-
669152
Lactobacillus pentosus
2-hydroxyvalerate + NAD+
-
-
-
?
2-oxobutyrate + NADH + H+
-
669152
Lactobacillus pentosus
2-hydroxybutyrate + NAD+
-
-
-
?
2-oxoglutarate + NADH + H+
-
669152
Lactobacillus pentosus
2-hydroxypentanedioate + NAD+
-
-
-
?
hydroxypyruvate + NADH
-
669152
Lactobacillus pentosus
glycerate + NAD+
-
-
-
?
oxaloacetate + NADH
-
669152
Lactobacillus pentosus
malate + NAD+
-
-
-
?
phenylpyruvate + NADH
-
669152
Lactobacillus pentosus
phenyllactate + NAD+
-
-
-
?
pyruvate + NADH + H+
-
669152
Lactobacillus pentosus
(R)-lactate + NAD+
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.056
-
2-ketocaproate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
0.3
-
2-ketocaproate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
0.31
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme Y52R
Lactobacillus pentosus
0.36
-
2-ketocaproate
30°C, pH 5.5, mutant enzyme Y52R/F299G
Lactobacillus pentosus
0.48
-
oxaloacetate
30°C, pH 5.5, mutant enzyme Y52R
Lactobacillus pentosus
0.51
-
2-Ketoglutarate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
0.7
-
2-ketocaproate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
2.1
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme F299S
Lactobacillus pentosus
2.8
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme Y52R/F299G
Lactobacillus pentosus
3.4
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme F299G
Lactobacillus pentosus
5.2
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme Y52T/D299S
Lactobacillus pentosus
5.7
-
2-Ketovalerate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
6.5
-
oxaloacetate
30°C, pH 5.5, mutant enzyme F299S
Lactobacillus pentosus
8.1
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme F299G
Lactobacillus pentosus
11.1
-
2-ketoisocaproate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
11.7
-
2-ketoisovalerate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
13.8
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme Y52R
Lactobacillus pentosus
15.8
-
2-ketoisocaproate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
15.9
-
2-ketocaproate
30°C, pH 5.5, mutant enzyme Y52R
Lactobacillus pentosus
17
-
2-ketoisocaproate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
19
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme Y52R/F299G
Lactobacillus pentosus
21
-
2-ketocaproate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
22
-
pyruvate
30°C, pH 5.5, mutant enzyme F299G
Lactobacillus pentosus
25.5
-
pyruvate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
27
-
2-ketoisovalerate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
35.8
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme F299S
Lactobacillus pentosus
37
-
pyruvate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
40
-
phenylpyruvate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
41
-
2-Ketobutyrate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
41.5
-
2-Ketobutyrate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
50
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
55.7
-
2-Ketovalerate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
65.7
-
2-ketoisocaproate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
102
-
2-Ketovalerate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
104
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
118
-
2-Ketobutyrate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
171
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme Y52V
Lactobacillus pentosus
175
-
2-Ketobutyrate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
213
-
pyruvate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
232
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme Y52A
Lactobacillus pentosus
257
-
Hydroxypyruvate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
280
-
oxaloacetate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
321
-
pyruvate
30°C, pH 5.5, wild-type enzyme
Lactobacillus pentosus
334
-
2-Ketovalerate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
407
-
Hydroxypyruvate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
778
-
phenylpyruvate
30°C, pH 5.5, mutant enzyme Y52L
Lactobacillus pentosus
Other publictions for EC 1.1.1.28
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723678
Jun
-
Discovery and characterization ...
Lactobacillus jensenii
Process Biochem.
48
109-117
2013
-
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1
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6
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1
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1
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15
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6
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2
2
4
6
2
2
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2
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1
2
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6
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1
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15
-
6
-
2
2
4
6
2
2
-
-
-
-
-
-
6
6
721879
Yu
Characterization of D-lactate ...
Pediococcus pentosaceus, Pediococcus pentosaceus ATCC 25745
Biosci. Biotechnol. Biochem.
76
853-855
2012
-
-
-
-
-
-
3
2
-
4
1
4
-
4
-
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2
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4
1
1
1
1
2
1
1
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2
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2
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3
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2
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4
1
4
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2
-
4
1
1
1
1
2
1
1
-
-
-
-
-
-
2
2
721909
Mu
Characterization of D-lactate ...
Pediococcus acidilactici, Pediococcus acidilactici DSM 20284
Biotechnol. Lett.
34
907-911
2012
-
-
1
-
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2
2
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2
1
4
-
2
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1
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2
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4
1
1
1
1
1
1
1
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2
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1
2
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2
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2
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2
1
4
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1
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2
-
4
1
1
1
1
1
1
1
-
-
-
-
-
-
2
2
722140
Li
Characterization of the major ...
Leuconostoc mesenteroides subsp. mesenteroides, Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293
Enzyme Microb. Technol.
51
274-279
2012
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1
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3
4
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2
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7
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1
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1
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1
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2
2
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1
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1
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3
-
2
1
2
1
-
2
2
2
-
-
-
-
-
-
-
-
722283
Wienstroer
D-Lactate dehydrogenase as a m ...
Arabidopsis thaliana, Arabidopsis thaliana Columbia-0
FEBS Lett.
586
36-40
2012
-
1
-
-
1
-
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2
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5
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2
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2
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2
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1
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2
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1
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2
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2
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-
2
-
-
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-
-
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-
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-
-
721200
Shibahara
Crystallization and preliminar ...
Aeropyrum pernix
Acta Crystallogr. Sect. F
67
1425-1427
2011
-
-
1
1
-
-
-
-
-
-
2
2
-
1
-
-
1
-
-
-
-
-
2
1
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2
-
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1
2
1
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2
2
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-
1
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-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
701818
Okano
Improved production of homo-D- ...
Lactobacillus plantarum, Lactobacillus plantarum NCIMB 8826
Appl. Environ. Microbiol.
75
7858-7861
2009
-
-
-
-
1
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3
-
4
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3
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1
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3
-
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-
3
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
710727
Antonyuk
Structure of D-lactate dehydro ...
Aquifex aeolicus
Acta Crystallogr. Sect. F
65
1209-1213
2009
-
-
1
1
-
-
-
-
-
-
-
1
-
3
-
-
1
-
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-
3
1
-
-
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3
-
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1
3
1
-
-
-
-
-
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-
1
-
-
-
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
684633
Okino
Production of D-lactic acid by ...
Escherichia coli, Lactobacillus delbrueckii
Appl. Microbiol. Biotechnol.
78
449-454
2008
-
-
2
-
-
-
-
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4
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2
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5
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4
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2
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2
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4
-
2
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2
-
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
695707
Xu
Breeding of D(-)-lactic acid h ...
Sporolactobacillus sp., Sporolactobacillus sp. DX12
Appl. Biochem. Biotechnol.
160
314-321
2008
-
-
-
-
1
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-
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2
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2
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4
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1
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4
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-
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-
688878
Mazzei
-
Peroxidase based biosensors fo ...
Staphylococcus epidermidis
Microchem. J.
87
81-86
2007
-
1
-
-
1
-
-
-
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1
-
1
-
1
-
-
-
-
-
1
1
-
1
-
1
-
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-
1
-
-
2
-
-
-
-
1
-
2
-
1
-
-
-
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-
1
-
1
-
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-
1
1
-
1
-
1
-
-
-
1
-
-
-
-
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-
-
-
667751
Minenko
Use of the addressing sequence ...
Saccharomyces cerevisiae
Biochemistry
71
32-38
2006
-
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1
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2
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2
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-
-
-
-
669543
Ishida
D-lactic acid production by me ...
Leuconostoc mesenteroides
J. Biosci. Bioeng.
101
172-177
2006
-
1
1
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-
-
-
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5
-
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1
1
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-
-
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-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
665878
Fujisawa
-
D-lactate metabolism in starve ...
Octopus ocellatus
J. Exp. Zool. A
303A
489-496
2005
-
-
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2
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668233
Findrik
-
Modelling and optimization of ...
Lactobacillus leichmannii
Chem. Biochem. Eng. Q.
19
351-358
2005
-
1
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-
-
-
-
-
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1
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1
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1
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1
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1
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669152
Ishikura
Recognition site for the side ...
Lactobacillus pentosus
J. Biochem.
138
741-749
2005
-
-
-
-
9
-
-
46
-
-
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2
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10
-
-
-
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46
-
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1
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-
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-
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1
-
9
-
-
-
-
46
-
-
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-
-
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-
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10
-
-
-
-
46
-
-
-
-
-
-
-
-
-
-
669327
Shinoda
Distinct conformation-mediated ...
Lactobacillus pentosus
J. Biol. Chem.
280
17068-17075
2005
-
-
-
1
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2
8
-
-
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3
-
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-
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4
-
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-
8
-
-
-
-
4
-
-
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-
1
-
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2
4
8
-
-
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-
-
-
-
-
-
-
-
-
4
-
-
-
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8
-
-
-
-
-
-
-
-
-
-
670130
Zhu
Effect of a single-gene knocko ...
Escherichia coli
Metab. Eng.
7
104-115
2005
-
-
-
-
-
-
-
-
-
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1
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1
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1
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1
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1
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-
-
-
-
-
-
-
-
-
-
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-
654456
Hachiya
Interaction of D-lactate dehyd ...
Saccharomyces cerevisiae
Biochem. Biophys. Res. Commun.
319
78-82
2004
-
-
-
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1
-
-
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1
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2
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1
1
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1
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1
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-
-
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1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655064
Horikiri
Electron acquisition system co ...
Rhodopseudomonas palustris
Biosci. Biotechnol. Biochem.
68
516-522
2004
-
-
-
-
-
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1
1
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2
-
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3
-
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1
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1
-
3
1
1
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2
-
1
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2
1
1
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-
-
2
-
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-
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1
1
1
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2
-
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1
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1
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3
1
1
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2
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1
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1
-
-
-
-
-
-
656426
Iino
The effect of sodium acetate o ...
Lactobacillus plantarum, Lactobacillus plantarum NRIC 1067T, Lactobacillus sakei, Lactobacillus sakei NRIC 1071T
J. Gen. Appl. Microbiol.
49
51-58
2003
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2
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6
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-
-
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656314
Isobe
Crystallization and some prope ...
Staphylococcus sp., Staphylococcus sp. LDH-1
J. Biosci. Bioeng.
94
330-335
2002
-
-
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-
-
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3
4
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2
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3
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1
-
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1
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11
2
1
1
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2
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1
2
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2
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3
-
4
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2
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1
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1
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11
2
1
1
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2
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1
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-
656500
Razeto
Domain closure, substrate spec ...
Lactobacillus delbrueckii subsp. bulgaricus
J. Mol. Biol.
318
109-119
2002
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-
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1
1
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3
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1
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2
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2
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2
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2
1
1
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2
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2
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286501
Kochhar
Roles of His205, His296, His30 ...
Lactobacillus delbrueckii subsp. bulgaricus
Eur. J. Biochem.
267
1633-1639
2000
-
-
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4
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1
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1
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286502
Dym
The crystal structure of D-lac ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
97
9413-9418
2000
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1
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3
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3
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1
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286500
Stoll
A thioredoxin fusion protein o ...
Enterococcus faecium
Protein Sci.
7
1147-1155
1998
3
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1
1
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3
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1
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3
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1
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286499
Dartois
Purification, properties and D ...
Leuconostoc mesenteroides subsp. cremoris
Res. Microbiol.
146
291-302
1995
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1
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4
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1
1
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4
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1
1
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286503
Nessler
Crystallization of D-lactate d ...
Lactobacillus delbrueckii subsp. bulgaricus
J. Mol. Biol.
235
370-371
1994
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1
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1
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286498
Erwin
Oxidation of D-lactate and L-l ...
Neisseria meningitidis
J. Bacteriol.
175
6382-6391
1993
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1
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9
1
1
2
1
1
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3
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1
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1
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1
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9
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1
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1
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1
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286496
Kochhar
Primary structure, physicochem ...
Lactobacillus delbrueckii subsp. bulgaricus
J. Biol. Chem.
267
8499-8513
1992
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-
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1
1
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1
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286497
Kochhar
Cloning and overexpression of ...
Lactobacillus delbrueckii subsp. bulgaricus
Biochem. Biophys. Res. Commun.
185
705-712
1992
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1
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5
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1
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1
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286504
Kochhar
Glutamate 264 modulates the pH ...
Lactobacillus delbrueckii subsp. bulgaricus
J. Biol. Chem.
267
20298-20301
1992
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1
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1
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4
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4
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1
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659546
Schmidt
Optimization of a process for ...
Staphylococcus epidermidis
J. Biotechnol.
24
315-327
1992
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1
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1
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1
-
1
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1
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286495
Le Bras
Properties of D-lactate dehydr ...
Lactobacillus delbrueckii subsp. bulgaricus
FEMS Microbiol. Lett.
79
89-93
1991
-
-
-
-
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2
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1
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1
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286483
Jaenicke
-
Dimeric lactic dehydrogenase f ...
Cardium edule
Naturwissenschaften
68
205-206
1981
-
-
-
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2
1
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1
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286430
Garvie
Bacterial lactate dehydrogenas ...
Enterobacter aerogenes, Escherichia coli, Eubacterium limosum, Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus buchneri, Lactobacillus casei, Lactobacillus delbrueckii, Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus delbrueckii subsp. lactis, Lactobacillus fermentum, Lactobacillus jensenii, Lactobacillus jugurt-helveticus, Lactobacillus leichmannii, Lactobacillus plantarum, Lactobacillus vermiforme, Lactococcus lactis, Leuconostoc lactis, Leuconostoc mesenteroides, Leuconostoc mesenteroides subsp. cremoris, Leuconostoc mesenteroides subsp. dextranicum, Mammalia, Mycoplasma sp., Oenococcus oeni, Pediococcus acidilactici, Pediococcus damnosus, Pediococcus inopinatus, Pediococcus pentosaceus, Selenomonas ruminantium, Staphylococcus aureus, Staphylococcus haemolyticus, Staphylococcus hominis, Staphylococcus sp., Staphylococcus warneri, Weissella confusa, Weissella paramesenteroides, Weissella viridescens
Microbiol. Rev.
44
106-139
1980
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197
11
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37
-
37
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37
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15
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8
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74
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74
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197
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11
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37
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37
-
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15
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8
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286489
Long
-
Comparative enzymology and phy ...
Haliotis cracherodii, Helix aspersa
J. Exp. Zool.
207
237-248
1979
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-
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2
10
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2
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4
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286488
Storey
Lactate dehydrogenase in tissu ...
Helix aspersa
Comp. Biochem. Physiol. B
56
181-187
1977
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1
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1
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286484
LeJohn
D(-)-lactate dehydrogenase fro ...
Phytophthora undulata, Pythium debaryanum, Sapromyces elongatus
Methods Enzymol.
41B
293-298
1975
3
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3
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6
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6
3
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286490
Wittenberger
D(-)-Lactate dehydrogenase fro ...
Eubacterium limosum
Methods Enzymol.
41B
299-303
1975
-
-
-
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1
4
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1
1
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1
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1
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286491
Long
D-Lactate dehydrogenase from t ...
Limulus polyphemus
Methods Enzymol.
41B
313-323
1975
-
-
-
-
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1
4
4
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2
1
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1
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1
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1
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1
1
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1
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286492
Long
Diphosphopyridine nucleotide-l ...
Limulus polyphemus, Neanthes virens
Arch. Biochem. Biophys.
154
696-710
1973
-
-
-
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2
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2
2
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2
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2
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1
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2
1
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286493
Purohit
D(-)-lactate dehydrogenase fro ...
Allomyces sp.
Arch. Mikrobiol.
84
287-300
1972
-
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1
2
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1
1
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286485
Garland
Salt-induced alteration of D(- ...
Polysphondylium pallidum
Biochem. Biophys. Res. Commun.
26
679-685
1967
-
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1
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286486
Wolin
Fructose-1,6-diphosphate requi ...
Streptococcus equinus
Science
146
775-776
1964
1
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1
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-
286487
Pascal
-
Sur la configuration du lactat ...
Enterobacter aerogenes
Ann. Inst. Pasteur (Paris)
107
55-62
1964
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