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Literature summary for 1.1.1.276 extracted from

  • Chowdhury, E.K.; Higuchi, K.; Nagata, S.; Misono, H.
    A novel NADP+ dependent serine dehydrogenase from Agrobacterium tumefaciens (1997), Biosci. Biotechnol. Biochem., 61, 152-157.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-methylmalonate competitive against L-serine, noncompetitive against NADP+ Agrobacterium tumefaciens
Co2+ weak Agrobacterium tumefaciens
D-cysteine competitive against L-serine, noncompetitive against NADP+ Agrobacterium tumefaciens
Hg2+ strong Agrobacterium tumefaciens
L-cysteine competitive against L-serine, noncompetitive against NADP+ Agrobacterium tumefaciens
malonate competitive against L-serine, noncompetitive against NADP+ Agrobacterium tumefaciens
additional information no inhibition by EDTA, sodium arsenate, o-phenethroline, alpha,alpha'-dipyridyl and very slight inhibition by sodium azide Agrobacterium tumefaciens
N-ethylmaleimide
-
Agrobacterium tumefaciens
p-chloromercuribenzoate strong Agrobacterium tumefaciens
Tartronate competitive against L-serine, noncompetitive against NADP+ Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
NADP+ pH 9.0, 30°C Agrobacterium tumefaciens
42
-
L-serine pH 9.0, 30 °C Agrobacterium tumefaciens
44
-
D-serine pH 9.0, 30 °C Agrobacterium tumefaciens
54
-
L-Glycerate pH 9.0, 30 °C Agrobacterium tumefaciens
56
-
D-glycerate pH 9.0, 30 °C Agrobacterium tumefaciens

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no effect with Cu2+, Mg2+, Mn2+, Zn2+, Fe2+ Agrobacterium tumefaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
gel filtration Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine + NADP+ Agrobacterium tumefaciens
-
2-aminomalonate semialdehyde + NADPH
-
?

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Agrobacterium tumefaciens

Reaction

Reaction Comment Organism Reaction ID
L-serine + NADP+ = 2-aminoacetaldehyde + CO2 + NADPH + H+ sequential mechanism Agrobacterium tumefaciens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
45.3
-
purified enzyme Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methyl-DL-serine + NADP+ 8.7% activity compared to L-serine Agrobacterium tumefaciens 2-amino-2-methyl-3-oxopropanoic acid + NADPH
-
?
3-hydroxypropanoate + NADP+ 1.6% activity compared to L-serine Agrobacterium tumefaciens 3-oxopropanoate + NADPH
-
?
D-3-hydroxyisobutyrate + NADP+ 3.5% activity compared to L-serine Agrobacterium tumefaciens 2-methyl-3-oxopropanoic acid + NADPH
-
?
D-glycerate + NADP+ 12.9% activity compared to L-serine Agrobacterium tumefaciens ?
-
?
D-serine + NADP+ 55.2% activity compared to L-serine Agrobacterium tumefaciens 2-aminomalonate semialdehyde + NADPH
-
?
L-3-hydroxyisobutyrate + NADP+ 8.5% activity compared to L-serine Agrobacterium tumefaciens 2-methyl-3-oxopropanoic acid + NADPH
-
?
L-glycerate + NADP+ 11.3% activity compared to L-serine Agrobacterium tumefaciens ?
-
?
L-serine + NADP+
-
Agrobacterium tumefaciens 2-aminomalonate semialdehyde + NADPH
-
?
L-serine + NADP+ best substrate Agrobacterium tumefaciens 2-aminomalonate semialdehyde + NADPH
-
?
additional information no activity with 4-hydroxybutyrate, DL-2-hydroxybutyrate, DL-2-hydroxyisobutyrate, DL-2-hydroxycaproate, DL-2-hydroxyisocaproate, 3-phosphoglycerate, DL-lactate, L-malate, glycollate, glycerol, D-threonine, DL-threo-phenylserine, DL-homoserine, DL-isoserine, L-alanine, beta-alanine, L-aspartate, L-glutamate, L-valine, L-leucine, L-methionine, L-lysine, L-phenylalanine, and glycine Agrobacterium tumefaciens ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Agrobacterium tumefaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
stable at Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.1
-
-
Agrobacterium tumefaciens

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 8 stable at Agrobacterium tumefaciens

Cofactor

Cofactor Comment Organism Structure
NADP+ dependent on Agrobacterium tumefaciens
NADP+ NAD+ cannot replace NADP+ Agrobacterium tumefaciens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.067
-
L-cysteine versus L-serine, pH 9.0, 30°C Agrobacterium tumefaciens
0.21
-
L-cysteine versus NADP+, pH 9.0, 30°C Agrobacterium tumefaciens
0.59
-
D-cysteine versus L-serine, pH 9.0, 30°C Agrobacterium tumefaciens
9.9
-
D-cysteine versus NADP+, pH 9.0, 30°C Agrobacterium tumefaciens
10
-
2-methylmalonate versus NADP+, pH 9.0, 30°C Agrobacterium tumefaciens
11
-
malonate versus L-serine, pH 9.0, 30°C Agrobacterium tumefaciens
12
-
2-methylmalonate versus L-serine, pH 9.0, 30°C Agrobacterium tumefaciens
13
-
Tartronate versus L-serine, pH 9.0, 30°C Agrobacterium tumefaciens
17
-
malonate versus NADP+, pH 9.0, 30°C Agrobacterium tumefaciens
18
-
Tartronate versus NADP+, pH 9.0, 30°C Agrobacterium tumefaciens