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Literature summary for 1.1.1.267 extracted from

  • Engprasert, S.; Taura, F.; Shoyama, Y.
    Molecular cloning, expression and characterization of recombinant 1-deoxy-D-xylulose-5-phosphate reductoisomerase from Coleus forskohlii Briq. (2005), Plant Sci., 169, 287-294.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, quantitative expression analysis, expression of the C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) periplasmic space Plectranthus barbatus

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Plectranthus barbatus
fosmidomycin IC50: 450 nM, recombinant enzyme, inhibition of growth and forskolin production in vivo Plectranthus barbatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0825
-
NADPH pH 8.0, 37°C, recombinant enzyme Plectranthus barbatus
0.147
-
1-deoxy-D-xylulose 5-phosphate pH 8.0, 37°C, recombinant enzyme Plectranthus barbatus

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Plectranthus barbatus 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates, a divalent cation is required for activity, can partially substitute for Mn2+ Plectranthus barbatus
Co2+ activates, a divalent cation is required for activity, can partially substitute for Mn2+ Plectranthus barbatus
Mg2+ activates, a divalent cation is required for activity, can partially substitute for Mn2+ Plectranthus barbatus
Mn2+ activates, a divalent cation is required for activity, best cation, can partially be substituted by other divalent cations Plectranthus barbatus
additional information no activity with Fe2+ and Cu2+ Plectranthus barbatus
Zn2+ activates, a divalent cation is required for activity, can partially substitute for Mn2+ Plectranthus barbatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
x * 54000, recombinant enzyme, SDS-PAGE Plectranthus barbatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Plectranthus barbatus the enzyme catalyzes the first committed step in the biosynthesis of isopentenyl diphosphate in the plastidial 2C-methyl-D-erythritol 4-phosphate pathway leading to production of forskolin, overview 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Plectranthus barbatus Q6R3F4 i.e. Plectranthus barbatus
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His-tagged enzyme 8.7fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultracentrifugation and dialysis, followed by anion exchange and hydroxylapatite chromatography, affinity chromatography, and again dialysis Plectranthus barbatus

Source Tissue

Source Tissue Comment Organism Textmining
leaf high expression level Plectranthus barbatus
-
additional information quantitative expression analysis Plectranthus barbatus
-
root very low expression level Plectranthus barbatus
-
stem moderate expression level Plectranthus barbatus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
quantitative analysis of forskolin production in plant tissues Plectranthus barbatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NAD(P)H + H+ NADPH is the preferred cofactor Plectranthus barbatus 2-C-methyl-D-erythritol 4-phosphate + NAD(P)+
-
?
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ the enzyme catalyzes the first committed step in the biosynthesis of isopentenyl diphosphate in the plastidial 2C-methyl-D-erythritol 4-phosphate pathway leading to production of forskolin, overview Plectranthus barbatus 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
?

Subunits

Subunits Comment Organism
? x * 54000, recombinant enzyme, SDS-PAGE Plectranthus barbatus

Synonyms

Synonyms Comment Organism
DXR
-
Plectranthus barbatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 45 recombinant enzyme Plectranthus barbatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8.3 recombinant enzyme Plectranthus barbatus

Cofactor

Cofactor Comment Organism Structure
NADH 15% of the activity with NADPH Plectranthus barbatus
NADPH preferred cofactor Plectranthus barbatus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00045
-
IC50: 450 nM, recombinant enzyme, inhibition of growth and forskolin production in vivo Plectranthus barbatus fosmidomycin