Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, quantitative expression analysis, expression of the C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) periplasmic space | Plectranthus barbatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Plectranthus barbatus | |
fosmidomycin | IC50: 450 nM, recombinant enzyme, inhibition of growth and forskolin production in vivo | Plectranthus barbatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0825 | - |
NADPH | pH 8.0, 37°C, recombinant enzyme | Plectranthus barbatus | |
0.147 | - |
1-deoxy-D-xylulose 5-phosphate | pH 8.0, 37°C, recombinant enzyme | Plectranthus barbatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Plectranthus barbatus | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates, a divalent cation is required for activity, can partially substitute for Mn2+ | Plectranthus barbatus | |
Co2+ | activates, a divalent cation is required for activity, can partially substitute for Mn2+ | Plectranthus barbatus | |
Mg2+ | activates, a divalent cation is required for activity, can partially substitute for Mn2+ | Plectranthus barbatus | |
Mn2+ | activates, a divalent cation is required for activity, best cation, can partially be substituted by other divalent cations | Plectranthus barbatus | |
additional information | no activity with Fe2+ and Cu2+ | Plectranthus barbatus | |
Zn2+ | activates, a divalent cation is required for activity, can partially substitute for Mn2+ | Plectranthus barbatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
x * 54000, recombinant enzyme, SDS-PAGE | Plectranthus barbatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | Plectranthus barbatus | the enzyme catalyzes the first committed step in the biosynthesis of isopentenyl diphosphate in the plastidial 2C-methyl-D-erythritol 4-phosphate pathway leading to production of forskolin, overview | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plectranthus barbatus | Q6R3F4 | i.e. Plectranthus barbatus | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged enzyme 8.7fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultracentrifugation and dialysis, followed by anion exchange and hydroxylapatite chromatography, affinity chromatography, and again dialysis | Plectranthus barbatus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | high expression level | Plectranthus barbatus | - |
additional information | quantitative expression analysis | Plectranthus barbatus | - |
root | very low expression level | Plectranthus barbatus | - |
stem | moderate expression level | Plectranthus barbatus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
quantitative analysis of forskolin production in plant tissues | Plectranthus barbatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NAD(P)H + H+ | NADPH is the preferred cofactor | Plectranthus barbatus | 2-C-methyl-D-erythritol 4-phosphate + NAD(P)+ | - |
? | |
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | the enzyme catalyzes the first committed step in the biosynthesis of isopentenyl diphosphate in the plastidial 2C-methyl-D-erythritol 4-phosphate pathway leading to production of forskolin, overview | Plectranthus barbatus | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000, recombinant enzyme, SDS-PAGE | Plectranthus barbatus |
Synonyms | Comment | Organism |
---|---|---|
DXR | - |
Plectranthus barbatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 45 | recombinant enzyme | Plectranthus barbatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.3 | recombinant enzyme | Plectranthus barbatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | 15% of the activity with NADPH | Plectranthus barbatus | |
NADPH | preferred cofactor | Plectranthus barbatus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00045 | - |
IC50: 450 nM, recombinant enzyme, inhibition of growth and forskolin production in vivo | Plectranthus barbatus | fosmidomycin |