Application | Comment | Organism |
---|---|---|
drug development | the enzyme is an excellent drug target in a number of pathogenic organisms | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
gene Rv2870c, expression of His-tagged enzyme in Escherichia coli strain BL21 | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme with inhibitor fosmidomycin, microbatch method, sitting drops mixed from 0.001 ml of protein solution, containing 5 mg/ml protein in 150 mM NaCl and 20 mM TrisHCl pH 7.5, and 0.001 ml of crystallization buffer, containing 40% ethylene glycol, 2 mM MgSO4, 20 mM DTT, 0.2 mM EDTA and 100 mM acetate pH 5.0, streak-seeded immediately with a horse hair, 4 weeks, stabilization prior to flash-cooling in reservoir solution complemented with additional 10% ethylene glycol and 1 mM fosmidomycin, X-ray diffraction structure determination and analysis at 1.9 A resolution, molecular replacement | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fosmidomycin | - |
Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0072 | - |
NADPH | - |
Mycobacterium tuberculosis | |
0.34 | - |
1-deoxy-D-xylulose 5-phosphate | - |
Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | Mycobacterium tuberculosis | second step of the deoxyxylulose 5-phosphate/methylerythritol 4-phosphate pathway with end product isopentenylphosphate | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? | |
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | Mycobacterium tuberculosis H37Rv | second step of the deoxyxylulose 5-phosphate/methylerythritol 4-phosphate pathway with end product isopentenylphosphate | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
gene Rv2870c | - |
Mycobacterium tuberculosis H37Rv | - |
gene Rv2870c | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 by metal ion affinity chromatography, and gel filtration, to 98% purity | Mycobacterium tuberculosis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | active site structure | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | - |
Mycobacterium tuberculosis | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? | |
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | second step of the deoxyxylulose 5-phosphate/methylerythritol 4-phosphate pathway with end product isopentenylphosphate | Mycobacterium tuberculosis | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? | |
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | - |
Mycobacterium tuberculosis H37Rv | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? | |
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | second step of the deoxyxylulose 5-phosphate/methylerythritol 4-phosphate pathway with end product isopentenylphosphate | Mycobacterium tuberculosis H37Rv | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | enzyme crystal structure, the dimer interface is created by interactions between the catalytic and connecting regions of each subunit, a sulfate is bound at the expected site of the phosphate moiety of the sugar substrate, molecular replacement structure analysis and comparison, overview | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
DXR | - |
Mycobacterium tuberculosis |
Rv2870c | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.3 | - |
NADPH | - |
Mycobacterium tuberculosis | |
5.3 | - |
1-deoxy-D-xylulose 5-phosphate | - |
Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Mycobacterium tuberculosis |