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Literature summary for 1.1.1.267 extracted from

  • Argyrou, A.; Blanchard, J.S.
    Kinetic and chemical mechanism of Mycobacterium tuberculosis 1-deoxy-D-xylulose-5-phosphate isomeroreductase (2004), Biochemistry, 43, 4375-4384.
    View publication on PubMed
Search for more literature for 1.1.1.267

Cloned(Commentary)

Cloned (Comment) Organism
-
 Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
additional information removal of C-terminal 18 amino acid residues has no effect on enzyme activity, affinity for substrates or sensitivity to isotopic probes Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
 NADPH presence of Co2+, pH 7.5, 25°C Mycobacterium tuberculosis
0.0033
-
 NADPH presence of Mn2+, pH 7.5, 25°C Mycobacterium tuberculosis
0.004
-
 1-deoxy-D-xylulose-5-phosphate cosubstrate NADPH, presence of Co2+, pH 7.5, 25°C Mycobacterium tuberculosis
0.005
-
 NADPH presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis
0.042
-
 1-deoxy-D-xylulose-5-phosphate cosubstrate NADPH, presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis
0.042
-
 methyl-D-erythritol-4-phosphate presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis
0.05
-
 1-deoxy-D-xylulose-5-phosphate cosubstrate NADH, presence of Co2+, pH 7.5, 25°C Mycobacterium tuberculosis
0.05
-
 NADH presence of Co2+, pH 7.5, 25°C Mycobacterium tuberculosis
0.1
-
 1-deoxy-D-xylulose-5-phosphate cosubstrate NADPH, presence of Mn2+, pH 7.5, 25°C Mycobacterium tuberculosis
0.17
-
 NADP+ presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis
0.21
-
 1-deoxy-D-xylulose-5-phosphate cosubstrate NADH, presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis
0.24
-
 1-deoxy-D-xylulose-5-phosphate cosubstrate NADH, presence of Mn2+, pH 7.5, 25°C Mycobacterium tuberculosis
0.26
-
 NADH presence of Mn2+, pH 7.5, 25°C Mycobacterium tuberculosis
0.41
-
 NADH presence of Mg2+, pH 7.5, 25°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ required, Km-value 0.0012 mM Mycobacterium tuberculosis
Mg2+ can partly substitute for Co2+, Km-value 1.2 mM Mycobacterium tuberculosis
Mn2+ can poorly replace for Co2+, Km-value 2.4 mM Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
 recombinant enzyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Mycobacterium tuberculosis

Reaction

Reaction Comment Organism Reaction ID
2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ steady-state random mechanism Mycobacterium tuberculosis
a

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, fully active for over 12 months Mycobacterium tuberculosis
4°C, half-life about 3 days Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-deoxy-D-xylulose-5-phosphate + NADH
-
 Mycobacterium tuberculosis methyl-D-erythritol-4-phosphate + NAD+
-
 r
1-deoxy-D-xylulose-5-phosphate + NADPH
-
 Mycobacterium tuberculosis methyl-D-erythritol-4-phosphate + NADP+
-
 r
methyl-D-erythritol-4-phosphate + NADP+
-
 Mycobacterium tuberculosis 1-deoxy-D-xylulose-5-phosphate + NADPH
-
 r

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Mycobacterium tuberculosis
NADH
-
 Mycobacterium tuberculosis
NADP+
-
 Mycobacterium tuberculosis
NADPH
-
 Mycobacterium tuberculosis