Application | Comment | Organism |
---|---|---|
synthesis | preparation of sulcatol enantiomers | Saccharomyces cerevisiae |
synthesis | preparation of sulcatol enantiomers | Aspergillus niger |
synthesis | preparation of sulcatol enantiomers | Geotrichum candidum |
synthesis | preparation of sulcatol enantiomers | Clostridium tyrobutyricum |
synthesis | preparation of sulcatol enantiomers | Gnathotrichus sulcatus |
synthesis | preparation of sulcatol enantiomers | Gnathotrichus retusus |
synthesis | preparation of sulcatol enantiomers | Thermoanaerobacter brockii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-methylhept-5-en-2-one + NADH | Thermoanaerobacter brockii | sulcatone | 6-methylhept-5-en-2-ol + NAD+ | 100% enantiomeric excess for S(+)-sulcatol | r | |
6-methylhept-5-en-2-one + NADH | Geotrichum candidum | sulcatone | 6-methylhept-5-en-2-ol + NAD+ | 96% sulcatol R enantiomer after 17 h reaction time | r | |
6-methylhept-5-en-2-one + NADH | Gnathotrichus retusus | sulcatone | 6-methylhept-5-en-2-ol + NAD+ | only sensitive to S enantiomer, inhibited by the R enantiomer | r | |
6-methylhept-5-en-2-one + NADH | Saccharomyces cerevisiae | sulcatone | 6-methylhept-5-en-2-ol + NAD+ | sulcatol | r | |
6-methylhept-5-en-2-one + NADH | Aspergillus niger | sulcatone | 6-methylhept-5-en-2-ol + NAD+ | sulcatol | r | |
6-methylhept-5-en-2-one + NADH | Geotrichum candidum | sulcatone | 6-methylhept-5-en-2-ol + NAD+ | sulcatol | r | |
6-methylhept-5-en-2-one + NADH | Clostridium tyrobutyricum | sulcatone | 6-methylhept-5-en-2-ol + NAD+ | sulcatol | r | |
6-methylhept-5-en-2-one + NADH | Gnathotrichus sulcatus | sulcatone | 6-methylhept-5-en-2-ol + NAD+ | sulcatol | r | |
6-methylhept-5-en-2-one + NADH | Gnathotrichus retusus | sulcatone | 6-methylhept-5-en-2-ol + NAD+ | sulcatol | r | |
6-methylhept-5-en-2-one + NADH | Thermoanaerobacter brockii | sulcatone | 6-methylhept-5-en-2-ol + NAD+ | sulcatol | r | |
6-methylhept-5-en-2-one + NADH | Saccharomyces cerevisiae | sulcatone | 6-methylhept-5-en-2-ol + NAD+ | S enantiomer formed | r | |
6-methylhept-5-en-2-one + NADH | Thermoanaerobacter brockii | sulcatone | 6-methylhept-5-en-2-ol + NAD+ | S enantiomer formed | r | |
6-methylhept-5-en-2-one + NADH | Gnathotrichus sulcatus | sulcatone | 6-methylhept-5-en-2-ol + NAD+ | mixture of 65% S(+)- and 35% S(-)-sulcatol isomers | r | |
6-methylhept-5-en-2-one + NADH | Clostridium tyrobutyricum | sulcatone | 6-methylhept-5-en-2-ol + NAD+ | 80% of sulcatol R enantiomer after 48 h reaction time | r | |
6-methylhept-5-en-2-one + NADH | Aspergillus niger | sulcatone | 6-methylhept-5-en-2-ol + NAD+ | 80% sulcatol R(-) enantiomer after 24 h reaction time | r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Clostridium tyrobutyricum | - |
- |
- |
Geotrichum candidum | - |
- |
- |
Gnathotrichus retusus | - |
- |
- |
Gnathotrichus sulcatus | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Thermoanaerobacter brockii | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Saccharomyces cerevisiae | - |
cell culture | - |
Aspergillus niger | - |
cell culture | - |
Geotrichum candidum | - |
cell culture | - |
Clostridium tyrobutyricum | - |
cell culture | - |
Gnathotrichus sulcatus | - |
cell culture | - |
Gnathotrichus retusus | - |
cell culture | - |
Thermoanaerobacter brockii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-methylhept-5-en-2-one + NADH | sulcatone | Thermoanaerobacter brockii | 6-methylhept-5-en-2-ol + NAD+ | 100% enantiomeric excess for S(+)-sulcatol | r | |
6-methylhept-5-en-2-one + NADH | sulcatone | Geotrichum candidum | 6-methylhept-5-en-2-ol + NAD+ | 96% sulcatol R enantiomer after 17 h reaction time | r | |
6-methylhept-5-en-2-one + NADH | sulcatone | Gnathotrichus retusus | 6-methylhept-5-en-2-ol + NAD+ | only sensitive to S enantiomer, inhibited by the R enantiomer | r | |
6-methylhept-5-en-2-one + NADH | sulcatone | Saccharomyces cerevisiae | 6-methylhept-5-en-2-ol + NAD+ | sulcatol | r | |
6-methylhept-5-en-2-one + NADH | sulcatone | Aspergillus niger | 6-methylhept-5-en-2-ol + NAD+ | sulcatol | r | |
6-methylhept-5-en-2-one + NADH | sulcatone | Geotrichum candidum | 6-methylhept-5-en-2-ol + NAD+ | sulcatol | r | |
6-methylhept-5-en-2-one + NADH | sulcatone | Clostridium tyrobutyricum | 6-methylhept-5-en-2-ol + NAD+ | sulcatol | r | |
6-methylhept-5-en-2-one + NADH | sulcatone | Gnathotrichus sulcatus | 6-methylhept-5-en-2-ol + NAD+ | sulcatol | r | |
6-methylhept-5-en-2-one + NADH | sulcatone | Gnathotrichus retusus | 6-methylhept-5-en-2-ol + NAD+ | sulcatol | r | |
6-methylhept-5-en-2-one + NADH | sulcatone | Thermoanaerobacter brockii | 6-methylhept-5-en-2-ol + NAD+ | sulcatol | r | |
6-methylhept-5-en-2-one + NADH | sulcatone | Saccharomyces cerevisiae | 6-methylhept-5-en-2-ol + NAD+ | S enantiomer formed | r | |
6-methylhept-5-en-2-one + NADH | sulcatone | Thermoanaerobacter brockii | 6-methylhept-5-en-2-ol + NAD+ | S enantiomer formed | r | |
6-methylhept-5-en-2-one + NADH | sulcatone | Gnathotrichus sulcatus | 6-methylhept-5-en-2-ol + NAD+ | mixture of 65% S(+)- and 35% S(-)-sulcatol isomers | r | |
6-methylhept-5-en-2-one + NADH | sulcatone | Clostridium tyrobutyricum | 6-methylhept-5-en-2-ol + NAD+ | 80% of sulcatol R enantiomer after 48 h reaction time | r | |
6-methylhept-5-en-2-one + NADH | sulcatone | Aspergillus niger | 6-methylhept-5-en-2-ol + NAD+ | 80% sulcatol R(-) enantiomer after 24 h reaction time | r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Saccharomyces cerevisiae | |
NADH | - |
Aspergillus niger | |
NADH | - |
Geotrichum candidum | |
NADH | - |
Clostridium tyrobutyricum | |
NADH | - |
Gnathotrichus sulcatus | |
NADH | - |
Gnathotrichus retusus | |
NADH | - |
Thermoanaerobacter brockii | |
NADPH | - |
Clostridium tyrobutyricum | |
NADPH | - |
Thermoanaerobacter brockii |