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Literature summary for 1.1.1.26 extracted from

  • Ohshima, T.; Nunoura-Kominato, N.; Kudome, T.; Sakuraba, H.
    A novel hyperthermophilic archaeal glyoxylate reductase from Thermococcus litoralis. Characterization, gene cloning, nucleotide sequence and expression in Escherichia coli (2001), Eur. J. Biochem., 268, 4740-4747.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned and expressed in Escherichia coli Thermococcus litoralis

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate 1 mM, 95% inhibition Thermococcus litoralis
CuCl2 1 mM, 66% inhibition Thermococcus litoralis
dithiothreitol 1 mM, 50% inhibition Thermococcus litoralis
HgCl2 1 mM, complete inhibition Thermococcus litoralis
additional information a significant effect on the activity is not observed with 1 mM EDTA, 1 mM iodoacetate and 1 mM 2-mercaptoethanol Thermococcus litoralis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.067
-
NADH pH 6.5, 50°C Thermococcus litoralis
0.73
-
glyoxylate pH 6.5, 50°C Thermococcus litoralis
1.3
-
Hydroxypyruvate pH 6.5, 50°C Thermococcus litoralis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36807
-
2 * 36807, calculated from sequence Thermococcus litoralis
37000
-
2 * 37000, SDS-PAGE Thermococcus litoralis
76000
-
non-denaturimg gradient PAGE Thermococcus litoralis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycolate + NAD+ Thermococcus litoralis
-
glyoxylate + NADH + H+
-
?
glycolate + NAD+ Thermococcus litoralis DSM 5473
-
glyoxylate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Thermococcus litoralis Q9C4M5
-
-
Thermococcus litoralis DSM 5473 Q9C4M5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermococcus litoralis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
44.5
-
pH 6.5, 50°C Thermococcus litoralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycolate + NAD+
-
Thermococcus litoralis glyoxylate + NADH + H+
-
?
glycolate + NAD+
-
Thermococcus litoralis DSM 5473 glyoxylate + NADH + H+
-
?
glyoxylate + NADH + H+ NADPH exhibits 7.5% of the activity compared to NADH Thermococcus litoralis glycolate + NAD+
-
?
glyoxylate + NADH + H+ NADPH exhibits 7.5% of the activity compared to NADH Thermococcus litoralis DSM 5473 glycolate + NAD+
-
?
hydroxypyruvate + NADH + H+ the relative activity for hydroxypyruvate is one-quarter that of glyoxylate in the presence of NADH as an electron donor Thermococcus litoralis D-glycerate + NAD+
-
?
hydroxypyruvate + NADH + H+ the relative activity for hydroxypyruvate is one-quarter that of glyoxylate in the presence of NADH as an electron donor Thermococcus litoralis DSM 5473 D-glycerate + NAD+
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 37000, SDS-PAGE Thermococcus litoralis
homodimer 2 * 36807, calculated from sequence Thermococcus litoralis

Synonyms

Synonyms Comment Organism
NADH-dependent glyoxylate reductase
-
Thermococcus litoralis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
assay at Thermococcus litoralis
90
-
-
Thermococcus litoralis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
10 min, stable up to Thermococcus litoralis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Thermococcus litoralis

Cofactor

Cofactor Comment Organism Structure
NADH NADPH exhibits rather low activity as an electron donor compared with NADH Thermococcus litoralis