BRENDA - Enzyme Database show
show all sequences of 1.1.1.26

A novel hyperthermophilic archaeal glyoxylate reductase from Thermococcus litoralis. Characterization, gene cloning, nucleotide sequence and expression in Escherichia coli

Ohshima, T.; Nunoura-Kominato, N.; Kudome, T.; Sakuraba, H.; Eur. J. Biochem. 268, 4740-4747 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
cloned and expressed in Escherichia coli
Thermococcus litoralis
Inhibitors
Inhibitors
Commentary
Organism
Structure
4-chloromercuribenzoate
1 mM, 95% inhibition
Thermococcus litoralis
CuCl2
1 mM, 66% inhibition
Thermococcus litoralis
dithiothreitol
1 mM, 50% inhibition
Thermococcus litoralis
HgCl2
1 mM, complete inhibition
Thermococcus litoralis
additional information
a significant effect on the activity is not observed with 1 mM EDTA, 1 mM iodoacetate and 1 mM 2-mercaptoethanol
Thermococcus litoralis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.067
-
NADH
pH 6.5, 50°C
Thermococcus litoralis
0.73
-
glyoxylate
pH 6.5, 50°C
Thermococcus litoralis
1.3
-
Hydroxypyruvate
pH 6.5, 50°C
Thermococcus litoralis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36807
-
2 * 36807, calculated from sequence
Thermococcus litoralis
37000
-
2 * 37000, SDS-PAGE
Thermococcus litoralis
76000
-
non-denaturimg gradient PAGE
Thermococcus litoralis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glycolate + NAD+
Thermococcus litoralis
-
glyoxylate + NADH + H+
-
-
?
glycolate + NAD+
Thermococcus litoralis DSM 5473
-
glyoxylate + NADH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermococcus litoralis
Q9C4M5
-
-
Thermococcus litoralis DSM 5473
Q9C4M5
-
-
Purification (Commentary)
Commentary
Organism
-
Thermococcus litoralis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
44.5
-
pH 6.5, 50°C
Thermococcus litoralis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycolate + NAD+
-
727414
Thermococcus litoralis
glyoxylate + NADH + H+
-
-
-
?
glycolate + NAD+
-
727414
Thermococcus litoralis DSM 5473
glyoxylate + NADH + H+
-
-
-
?
glyoxylate + NADH + H+
NADPH exhibits 7.5% of the activity compared to NADH
727414
Thermococcus litoralis
glycolate + NAD+
-
-
-
?
glyoxylate + NADH + H+
NADPH exhibits 7.5% of the activity compared to NADH
727414
Thermococcus litoralis DSM 5473
glycolate + NAD+
-
-
-
?
hydroxypyruvate + NADH + H+
the relative activity for hydroxypyruvate is one-quarter that of glyoxylate in the presence of NADH as an electron donor
727414
Thermococcus litoralis
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADH + H+
the relative activity for hydroxypyruvate is one-quarter that of glyoxylate in the presence of NADH as an electron donor
727414
Thermococcus litoralis DSM 5473
D-glycerate + NAD+
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 36807, calculated from sequence; 2 * 37000, SDS-PAGE
Thermococcus litoralis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
assay at
Thermococcus litoralis
90
-
-
Thermococcus litoralis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
90
-
10 min, stable up to
Thermococcus litoralis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Thermococcus litoralis
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
NADPH exhibits rather low activity as an electron donor compared with NADH
Thermococcus litoralis
Cloned(Commentary) (protein specific)
Commentary
Organism
cloned and expressed in Escherichia coli
Thermococcus litoralis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
NADPH exhibits rather low activity as an electron donor compared with NADH
Thermococcus litoralis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
4-chloromercuribenzoate
1 mM, 95% inhibition
Thermococcus litoralis
CuCl2
1 mM, 66% inhibition
Thermococcus litoralis
dithiothreitol
1 mM, 50% inhibition
Thermococcus litoralis
HgCl2
1 mM, complete inhibition
Thermococcus litoralis
additional information
a significant effect on the activity is not observed with 1 mM EDTA, 1 mM iodoacetate and 1 mM 2-mercaptoethanol
Thermococcus litoralis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.067
-
NADH
pH 6.5, 50°C
Thermococcus litoralis
0.73
-
glyoxylate
pH 6.5, 50°C
Thermococcus litoralis
1.3
-
Hydroxypyruvate
pH 6.5, 50°C
Thermococcus litoralis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36807
-
2 * 36807, calculated from sequence
Thermococcus litoralis
37000
-
2 * 37000, SDS-PAGE
Thermococcus litoralis
76000
-
non-denaturimg gradient PAGE
Thermococcus litoralis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glycolate + NAD+
Thermococcus litoralis
-
glyoxylate + NADH + H+
-
-
?
glycolate + NAD+
Thermococcus litoralis DSM 5473
-
glyoxylate + NADH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thermococcus litoralis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
44.5
-
pH 6.5, 50°C
Thermococcus litoralis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycolate + NAD+
-
727414
Thermococcus litoralis
glyoxylate + NADH + H+
-
-
-
?
glycolate + NAD+
-
727414
Thermococcus litoralis DSM 5473
glyoxylate + NADH + H+
-
-
-
?
glyoxylate + NADH + H+
NADPH exhibits 7.5% of the activity compared to NADH
727414
Thermococcus litoralis
glycolate + NAD+
-
-
-
?
glyoxylate + NADH + H+
NADPH exhibits 7.5% of the activity compared to NADH
727414
Thermococcus litoralis DSM 5473
glycolate + NAD+
-
-
-
?
hydroxypyruvate + NADH + H+
the relative activity for hydroxypyruvate is one-quarter that of glyoxylate in the presence of NADH as an electron donor
727414
Thermococcus litoralis
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADH + H+
the relative activity for hydroxypyruvate is one-quarter that of glyoxylate in the presence of NADH as an electron donor
727414
Thermococcus litoralis DSM 5473
D-glycerate + NAD+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 36807, calculated from sequence; 2 * 37000, SDS-PAGE
Thermococcus litoralis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
assay at
Thermococcus litoralis
90
-
-
Thermococcus litoralis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
90
-
10 min, stable up to
Thermococcus litoralis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Thermococcus litoralis
Other publictions for EC 1.1.1.26
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722899
Ching
Glyoxylate reductase isoform 1 ...
Arabidopsis thaliana
J. Integr. Plant Biol.
54
152-168
2012
-
-
1
-
-
-
-
-
4
-
-
2
-
3
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
4
-
-
-
-
-
-
-
4
-
-
2
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
715548
Nunn
Metabolism of pentose sugars i ...
Sulfolobus solfataricus, Sulfolobus solfataricus P2
J. Biol. Chem.
285
33701-33709
2010
-
-
1
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
696125
Allan
Role of plant glyoxylate reduc ...
Arabidopsis thaliana
Biochem. J.
423
15-22
2009
-
-
-
-
1
-
-
-
2
-
-
3
-
3
-
-
-
-
-
3
-
-
4
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
2
-
-
3
-
-
-
-
-
3
-
-
4
1
-
-
-
-
-
-
-
-
1
2
2
1
-
-
684838
Shinoda
A highly specific glyoxylate r ...
Paracoccus sp.
Biochem. Biophys. Res. Commun.
355
782-787
2007
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
1
-
-
1
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
685349
Fauvart
Identification of a novel glyo ...
Rhizobium etli, Rhizobium etli CFN42
Biochim. Biophys. Acta
1774
1092-1098
2007
-
-
1
-
-
-
-
4
-
-
3
6
-
6
-
-
1
-
-
-
-
-
11
1
1
-
-
4
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
4
-
-
3
6
-
-
-
1
-
-
-
-
11
1
1
-
-
4
1
1
-
-
-
-
-
-
-
-
685901
Hoover
Characteristics of an Arabidop ...
Arabidopsis thaliana
Can. J. Bot.
85
883-895
2007
-
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
1
-
-
3
1
1
2
1
1
1
-
2
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
3
1
1
2
1
1
1
-
2
1
1
-
-
-
-
-
-
-
-
655702
Cregeen
Molecular analysis of the glyo ...
Homo sapiens
Hum. Mutat.
22
497
2003
-
1
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727414
Ohshima
A novel hyperthermophilic arch ...
Thermococcus litoralis, Thermococcus litoralis DSM 5473
Eur. J. Biochem.
268
4740-4747
2001
-
-
1
-
-
-
5
3
-
-
3
2
-
10
-
-
1
-
-
-
1
-
6
1
2
-
1
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
5
-
3
-
-
3
2
-
-
-
1
-
-
1
-
6
1
2
-
1
-
1
-
-
-
-
-
-
-
-
-
286417
Rumsby
Identification and expression ...
Homo sapiens
Biochim. Biophys. Acta
1446
383-388
1999
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286416
Giafi
Kinetic analysis and tissue di ...
Homo sapiens
Ann. Clin. Biochem.
35
104-109
1998
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286418
Givan
The enzymic reduction of glyox ...
Hordeum vulgare, Nicotiana tabacum, Petroselinum crispum, Pisum sativum, Spinacia oleracea, Zea mays
Plant Physiol.
100
552-556
1992
-
-
-
-
1
-
6
5
9
-
5
7
-
10
-
-
1
-
-
5
2
-
12
2
-
-
-
-
-
2
-
6
-
-
-
-
-
-
6
-
1
-
-
6
-
5
9
-
5
7
-
-
-
1
-
5
2
-
12
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
286419
Betsche
Identification and characteria ...
Cyanophora paradoxa, Pisum sativum
Plant Physiol.
98
887-893
1992
-
-
-
-
-
-
4
-
5
-
-
6
-
6
-
-
2
1
-
1
10
-
8
-
-
-
-
-
2
1
-
4
-
-
-
-
-
-
4
-
-
-
-
4
-
-
5
-
-
6
-
-
-
2
-
1
10
-
8
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
286409
Yokota
Occurrence and subcellular dis ...
Euglena gracilis
Agric. Biol. Chem.
45
15-22
1981
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286408
Hullin
Glyoxylate reductase, two form ...
Pseudomonas fluorescens
Methods Enzymol.
41B
343-348
1975
-
-
-
-
-
-
2
-
-
-
1
-
-
2
-
-
1
-
-
-
3
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
1
-
-
-
-
1
-
-
3
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286414
Bergmeyer
-
Glyoxylate reductase ...
Spinacia oleracea
Methods Enzym. Anal. , 3rd Ed. (Bergmeyer, H. U. , ed. )
2
126-328
1974
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286410
Suzuki
Studies on peroxisomes. IV. in ...
Rattus norvegicus
J. Biochem.
73
1033-1038
1973
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286413
Cerff
Glyceraldehyde 3-phosphate deh ...
Sinapis alba
Plant Physiol.
51
76-81
1973
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286411
Zelitch
-
Glycolic acid oxidase and glyo ...
Nicotiana tabacum, Spinacia oleracea
Methods Enzymol.
1
528-535
1955
-
-
-
-
-
-
3
-
-
-
-
-
-
2
-
-
1
-
-
2
2
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
4
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
2
2
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286412
Zelitch
The isolation and action of cr ...
Nicotiana tabacum
J. Biol. Chem.
216
553-575
1955
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
1
1
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
286415
Zelitch
Oxidation and reduction of gly ...
Spinacia oleracea
J. Biol. Chem.
201
719-726
1953
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-