Cloned (Comment) | Organism |
---|---|
exclusive localization in the cytosol of transgenic Arabidopsis plants co-expressing GFP-GLYR1 and and Cherry-PTS1, a fusion protein consisting of the Cherry fluorescent protein linked to the PTS1 of the peroxisomal enzyme hydroxypyruvate reductase. Expression of N-terminal GFP-tagged or Myc-tagged GLYR1 in tobacco BY-2 cell cytosol. GFP- or Myc-tagged GLYR1 is competent, at least partially, for import into peroxisomes, since replacement of the C-terminal glutamate in GLYR1 with leucine, which yields a canonical PTS1 (i.e., a C-terminal small-basic-hydrophobic tripeptide motif), results in the modified fusion protein (GFPGLYR1-E to L and Myc-GLYR1-E to L) being dual localized to the cytosol and peroxisomes in BY-2 cells | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | GLYR1 is exclusively located in the cytosol | Arabidopsis thaliana | 5829 | - |
additional information | GLYR1 is not relocalized from the cytosol to peroxisomes in response to abiotic stress | Arabidopsis thaliana | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycolate + NAD+ | Arabidopsis thaliana | - |
glyoxylate + NADH + H+ | - |
r | |
glycolate + NADP+ | Arabidopsis thaliana | - |
glyoxylate + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9LSV0 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycolate + NAD+ | - |
Arabidopsis thaliana | glyoxylate + NADH + H+ | - |
r | |
glycolate + NADP+ | - |
Arabidopsis thaliana | glyoxylate + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
glyoxylate reductase isoform 1 | - |
Arabidopsis thaliana |
GLYR1 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Arabidopsis thaliana | |
NADH | - |
Arabidopsis thaliana | |
NADP+ | - |
Arabidopsis thaliana | |
NADPH | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
additional information | due to the glutamate at the -1 position, GLYR1 C-terminal tripeptide, -SRE, does not function as a type 1 peroxisomal targeting signal, PTS1. GLYR1 is not relocalized from the cytosol to peroxisomes in response to abiotic stress | Arabidopsis thaliana |
physiological function | GLYR1 scavenges succinic semialdehyde and glyoxylate that escape from mitochondria and peroxisomes, respectively | Arabidopsis thaliana |