BRENDA - Enzyme Database show
show all sequences of 1.1.1.26

Glyoxylate reductase isoform 1 is localized in the cytosol and not peroxisomes in plant cells

Ching, S.L.; Gidda, S.K.; Rochon, A.; van Cauwenberghe, O.R.; Shelp, B.J.; Mullen, R.T.; J. Integr. Plant Biol. 54, 152-168 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
exclusive localization in the cytosol of transgenic Arabidopsis plants co-expressing GFP-GLYR1 and and Cherry-PTS1, a fusion protein consisting of the Cherry fluorescent protein linked to the PTS1 of the peroxisomal enzyme hydroxypyruvate reductase. Expression of N-terminal GFP-tagged or Myc-tagged GLYR1 in tobacco BY-2 cell cytosol. GFP- or Myc-tagged GLYR1 is competent, at least partially, for import into peroxisomes, since replacement of the C-terminal glutamate in GLYR1 with leucine, which yields a canonical PTS1 (i.e., a C-terminal small-basic-hydrophobic tripeptide motif), results in the modified fusion protein (GFPGLYR1-E to L and Myc-GLYR1-E to L) being dual localized to the cytosol and peroxisomes in BY-2 cells
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
GLYR1 is exclusively located in the cytosol
Arabidopsis thaliana
5829
-
additional information
GLYR1 is not relocalized from the cytosol to peroxisomes in response to abiotic stress
Arabidopsis thaliana
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glycolate + NAD+
Arabidopsis thaliana
-
glyoxylate + NADH + H+
-
-
r
glycolate + NADP+
Arabidopsis thaliana
-
glyoxylate + NADPH + H+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9LSV0
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
seedling
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycolate + NAD+
-
722899
Arabidopsis thaliana
glyoxylate + NADH + H+
-
-
-
r
glycolate + NADP+
-
722899
Arabidopsis thaliana
glyoxylate + NADPH + H+
-
-
-
r
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Arabidopsis thaliana
NADH
-
Arabidopsis thaliana
NADP+
-
Arabidopsis thaliana
NADPH
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
exclusive localization in the cytosol of transgenic Arabidopsis plants co-expressing GFP-GLYR1 and and Cherry-PTS1, a fusion protein consisting of the Cherry fluorescent protein linked to the PTS1 of the peroxisomal enzyme hydroxypyruvate reductase. Expression of N-terminal GFP-tagged or Myc-tagged GLYR1 in tobacco BY-2 cell cytosol. GFP- or Myc-tagged GLYR1 is competent, at least partially, for import into peroxisomes, since replacement of the C-terminal glutamate in GLYR1 with leucine, which yields a canonical PTS1 (i.e., a C-terminal small-basic-hydrophobic tripeptide motif), results in the modified fusion protein (GFPGLYR1-E to L and Myc-GLYR1-E to L) being dual localized to the cytosol and peroxisomes in BY-2 cells
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Arabidopsis thaliana
NADH
-
Arabidopsis thaliana
NADP+
-
Arabidopsis thaliana
NADPH
-
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
GLYR1 is exclusively located in the cytosol
Arabidopsis thaliana
5829
-
additional information
GLYR1 is not relocalized from the cytosol to peroxisomes in response to abiotic stress
Arabidopsis thaliana
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glycolate + NAD+
Arabidopsis thaliana
-
glyoxylate + NADH + H+
-
-
r
glycolate + NADP+
Arabidopsis thaliana
-
glyoxylate + NADPH + H+
-
-
r
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
seedling
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycolate + NAD+
-
722899
Arabidopsis thaliana
glyoxylate + NADH + H+
-
-
-
r
glycolate + NADP+
-
722899
Arabidopsis thaliana
glyoxylate + NADPH + H+
-
-
-
r
General Information
General Information
Commentary
Organism
additional information
due to the glutamate at the -1 position, GLYR1 C-terminal tripeptide, -SRE, does not function as a type 1 peroxisomal targeting signal, PTS1. GLYR1 is not relocalized from the cytosol to peroxisomes in response to abiotic stress
Arabidopsis thaliana
physiological function
GLYR1 scavenges succinic semialdehyde and glyoxylate that escape from mitochondria and peroxisomes, respectively
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
additional information
due to the glutamate at the -1 position, GLYR1 C-terminal tripeptide, -SRE, does not function as a type 1 peroxisomal targeting signal, PTS1. GLYR1 is not relocalized from the cytosol to peroxisomes in response to abiotic stress
Arabidopsis thaliana
physiological function
GLYR1 scavenges succinic semialdehyde and glyoxylate that escape from mitochondria and peroxisomes, respectively
Arabidopsis thaliana
Other publictions for EC 1.1.1.26
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722899
Ching
Glyoxylate reductase isoform 1 ...
Arabidopsis thaliana
J. Integr. Plant Biol.
54
152-168
2012
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2
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715548
Nunn
Metabolism of pentose sugars i ...
Sulfolobus solfataricus, Sulfolobus solfataricus P2
J. Biol. Chem.
285
33701-33709
2010
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696125
Allan
Role of plant glyoxylate reduc ...
Arabidopsis thaliana
Biochem. J.
423
15-22
2009
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1
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2
1
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684838
Shinoda
A highly specific glyoxylate r ...
Paracoccus sp.
Biochem. Biophys. Res. Commun.
355
782-787
2007
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685349
Fauvart
Identification of a novel glyo ...
Rhizobium etli, Rhizobium etli CFN42
Biochim. Biophys. Acta
1774
1092-1098
2007
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1
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4
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6
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1
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11
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4
1
1
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685901
Hoover
Characteristics of an Arabidop ...
Arabidopsis thaliana
Can. J. Bot.
85
883-895
2007
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1
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655702
Cregeen
Molecular analysis of the glyo ...
Homo sapiens
Hum. Mutat.
22
497
2003
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1
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3
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727414
Ohshima
A novel hyperthermophilic arch ...
Thermococcus litoralis, Thermococcus litoralis DSM 5473
Eur. J. Biochem.
268
4740-4747
2001
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1
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3
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2
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10
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6
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1
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1
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286417
Rumsby
Identification and expression ...
Homo sapiens
Biochim. Biophys. Acta
1446
383-388
1999
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1
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1
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286416
Giafi
Kinetic analysis and tissue di ...
Homo sapiens
Ann. Clin. Biochem.
35
104-109
1998
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1
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1
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1
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1
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286418
Givan
The enzymic reduction of glyox ...
Hordeum vulgare, Nicotiana tabacum, Petroselinum crispum, Pisum sativum, Spinacia oleracea, Zea mays
Plant Physiol.
100
552-556
1992
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1
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6
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9
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7
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2
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2
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12
2
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2
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286419
Betsche
Identification and characteria ...
Cyanophora paradoxa, Pisum sativum
Plant Physiol.
98
887-893
1992
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4
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1
10
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8
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2
1
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286409
Yokota
Occurrence and subcellular dis ...
Euglena gracilis
Agric. Biol. Chem.
45
15-22
1981
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1
1
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286408
Hullin
Glyoxylate reductase, two form ...
Pseudomonas fluorescens
Methods Enzymol.
41B
343-348
1975
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2
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286414
Bergmeyer
-
Glyoxylate reductase ...
Spinacia oleracea
Methods Enzym. Anal. , 3rd Ed. (Bergmeyer, H. U. , ed. )
2
126-328
1974
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286410
Suzuki
Studies on peroxisomes. IV. in ...
Rattus norvegicus
J. Biochem.
73
1033-1038
1973
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286413
Cerff
Glyceraldehyde 3-phosphate deh ...
Sinapis alba
Plant Physiol.
51
76-81
1973
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286411
Zelitch
-
Glycolic acid oxidase and glyo ...
Nicotiana tabacum, Spinacia oleracea
Methods Enzymol.
1
528-535
1955
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3
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2
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286412
Zelitch
The isolation and action of cr ...
Nicotiana tabacum
J. Biol. Chem.
216
553-575
1955
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1
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1
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1
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1
1
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1
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1
1
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286415
Zelitch
Oxidation and reduction of gly ...
Spinacia oleracea
J. Biol. Chem.
201
719-726
1953
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