Cloned (Comment) | Organism |
---|---|
expression of His6-tagged enzyme in Escherichia coli srain BL21 | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Arabidopsis thaliana | |
0.0026 | - |
NADPH | pH 7.8, 22°C, recombinant His6-tagged enzyme | Arabidopsis thaliana | |
0.0045 | - |
glyoxylate | pH 7.8, 22°C, recombinant His6-tagged enzyme | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21 by PEG precipitation, nickel affinity and anion exchange chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
additional information | tissue-specific expression, overview | Arabidopsis thaliana | - |
root | low activity | Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.28 | - |
purified recombinant His6-tagged enzyme | Arabidopsis thaliana |
Storage Stability | Organism |
---|---|
-80°C, partially purified recombinant His6-tagged enzyme in an ammonium sulfate pellet, 6 months, stable | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + NADPH | a recombinant gamma hydroxybutyrate dehydrogenase, EC 1.1.1.61, exhibits high glyoxylate reductase activity with a 250fold higher preference for glyoxylate than with succinic semialdehyde, via an essentially irreversible, NADPH-based mechanism, overview | Arabidopsis thaliana | glycolate + NADP+ | - |
ir | |
additional information | the enzyme prefers succinic semialdehyde 100fold to formaldehyde, acetaldehyde, butyraldehyde, 2-carboxybenzaldehyde, glyoxal, methylglyoxal, phenylglyoxal, and phenylglyoxylate | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000-35000, recombinant His6-tagged enzyme, SDS-PAGE | Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
recombinant His6-tagged enzyme | Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 57.5 | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.1 | - |
NADPH | pH 7.8, 22°C, recombinant His6-tagged enzyme | Arabidopsis thaliana | |
13 | - |
glyoxylate | pH 7.8, 22°C, recombinant His6-tagged enzyme | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
recombinant His6-tagged enzyme | Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 12 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Arabidopsis thaliana |