BRENDA - Enzyme Database show
show all sequences of 1.1.1.26

Characteristics of an Arabidopsis glyoxylate reductase: general biochemical properties and substrate specificity for the recombinant protein, and developmental expression and implications for glyoxylate and succinic semialdehyde metabolism in planta

Hoover, G.J.; Van Cauwenberghe, O.R.; Breitkreuz, K.E.; Clark, S.M.; Merrill, A.R.; Shelp, B.J.; Can. J. Bot. 85, 883-895 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of His6-tagged enzyme in Escherichia coli srain BL21
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Arabidopsis thaliana
0.0026
-
NADPH
pH 7.8, 22°C, recombinant His6-tagged enzyme
Arabidopsis thaliana
0.0045
-
glyoxylate
pH 7.8, 22°C, recombinant His6-tagged enzyme
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21 by PEG precipitation, nickel affinity and anion exchange chromatography
Arabidopsis thaliana
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Arabidopsis thaliana
-
additional information
tissue-specific expression, overview
Arabidopsis thaliana
-
root
low activity
Arabidopsis thaliana
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.28
-
purified recombinant His6-tagged enzyme
Arabidopsis thaliana
Storage Stability
Storage Stability
Organism
-80°C, partially purified recombinant His6-tagged enzyme in an ammonium sulfate pellet, 6 months, stable
Arabidopsis thaliana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glyoxylate + NADPH
a recombinant gamma hydroxybutyrate dehydrogenase, EC 1.1.1.61, exhibits high glyoxylate reductase activity with a 250fold higher preference for glyoxylate than with succinic semialdehyde, via an essentially irreversible, NADPH-based mechanism, overview
685901
Arabidopsis thaliana
glycolate + NADP+
-
-
-
ir
additional information
the enzyme prefers succinic semialdehyde 100fold to formaldehyde, acetaldehyde, butyraldehyde, 2-carboxybenzaldehyde, glyoxal, methylglyoxal, phenylglyoxal, and phenylglyoxylate
685901
Arabidopsis thaliana
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 33000-35000, recombinant His6-tagged enzyme, SDS-PAGE
Arabidopsis thaliana
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
recombinant His6-tagged enzyme
Arabidopsis thaliana
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
5
57.5
-
Arabidopsis thaliana
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
8.1
-
NADPH
pH 7.8, 22°C, recombinant His6-tagged enzyme
Arabidopsis thaliana
13
-
glyoxylate
pH 7.8, 22°C, recombinant His6-tagged enzyme
Arabidopsis thaliana
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
recombinant His6-tagged enzyme
Arabidopsis thaliana
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
12
-
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of His6-tagged enzyme in Escherichia coli srain BL21
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Arabidopsis thaliana
0.0026
-
NADPH
pH 7.8, 22°C, recombinant His6-tagged enzyme
Arabidopsis thaliana
0.0045
-
glyoxylate
pH 7.8, 22°C, recombinant His6-tagged enzyme
Arabidopsis thaliana
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21 by PEG precipitation, nickel affinity and anion exchange chromatography
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Arabidopsis thaliana
-
additional information
tissue-specific expression, overview
Arabidopsis thaliana
-
root
low activity
Arabidopsis thaliana
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.28
-
purified recombinant His6-tagged enzyme
Arabidopsis thaliana
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, partially purified recombinant His6-tagged enzyme in an ammonium sulfate pellet, 6 months, stable
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glyoxylate + NADPH
a recombinant gamma hydroxybutyrate dehydrogenase, EC 1.1.1.61, exhibits high glyoxylate reductase activity with a 250fold higher preference for glyoxylate than with succinic semialdehyde, via an essentially irreversible, NADPH-based mechanism, overview
685901
Arabidopsis thaliana
glycolate + NADP+
-
-
-
ir
additional information
the enzyme prefers succinic semialdehyde 100fold to formaldehyde, acetaldehyde, butyraldehyde, 2-carboxybenzaldehyde, glyoxal, methylglyoxal, phenylglyoxal, and phenylglyoxylate
685901
Arabidopsis thaliana
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 33000-35000, recombinant His6-tagged enzyme, SDS-PAGE
Arabidopsis thaliana
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
recombinant His6-tagged enzyme
Arabidopsis thaliana
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
5
57.5
-
Arabidopsis thaliana
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
8.1
-
NADPH
pH 7.8, 22°C, recombinant His6-tagged enzyme
Arabidopsis thaliana
13
-
glyoxylate
pH 7.8, 22°C, recombinant His6-tagged enzyme
Arabidopsis thaliana
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
recombinant His6-tagged enzyme
Arabidopsis thaliana
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
12
-
Arabidopsis thaliana
Other publictions for EC 1.1.1.26
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722899
Ching
Glyoxylate reductase isoform 1 ...
Arabidopsis thaliana
J. Integr. Plant Biol.
54
152-168
2012
-
-
1
-
-
-
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-
4
-
-
2
-
3
-
-
-
-
-
3
-
-
2
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-
-
-
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-
-
4
-
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-
-
1
4
-
-
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-
-
-
-
4
-
-
2
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
715548
Nunn
Metabolism of pentose sugars i ...
Sulfolobus solfataricus, Sulfolobus solfataricus P2
J. Biol. Chem.
285
33701-33709
2010
-
-
1
-
-
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-
2
-
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-
-
2
-
-
-
-
-
1
-
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2
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1
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1
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1
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1
1
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2
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1
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2
-
1
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-
1
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-
-
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-
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-
-
-
696125
Allan
Role of plant glyoxylate reduc ...
Arabidopsis thaliana
Biochem. J.
423
15-22
2009
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-
-
-
1
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-
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2
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-
3
-
3
-
-
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3
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4
1
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-
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2
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2
-
1
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2
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-
3
-
-
-
-
-
3
-
-
4
1
-
-
-
-
-
-
-
-
1
2
2
1
-
-
684838
Shinoda
A highly specific glyoxylate r ...
Paracoccus sp.
Biochem. Biophys. Res. Commun.
355
782-787
2007
-
-
-
-
1
-
-
1
-
-
-
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2
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1
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1
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1
1
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1
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1
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1
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1
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-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
685349
Fauvart
Identification of a novel glyo ...
Rhizobium etli, Rhizobium etli CFN42
Biochim. Biophys. Acta
1774
1092-1098
2007
-
-
1
-
-
-
-
4
-
-
3
6
-
6
-
-
1
-
-
-
-
-
11
1
1
-
-
4
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
4
-
-
3
6
-
-
-
1
-
-
-
-
11
1
1
-
-
4
1
1
-
-
-
-
-
-
-
-
685901
Hoover
Characteristics of an Arabidop ...
Arabidopsis thaliana
Can. J. Bot.
85
883-895
2007
-
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
1
-
-
3
1
1
2
1
1
1
-
2
1
1
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1
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-
1
1
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-
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-
3
-
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-
1
-
3
1
1
2
1
1
1
-
2
1
1
-
-
-
-
-
-
-
-
655702
Cregeen
Molecular analysis of the glyo ...
Homo sapiens
Hum. Mutat.
22
497
2003
-
1
-
-
3
-
-
-
-
-
-
-
-
2
-
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1
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1
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3
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727414
Ohshima
A novel hyperthermophilic arch ...
Thermococcus litoralis, Thermococcus litoralis DSM 5473
Eur. J. Biochem.
268
4740-4747
2001
-
-
1
-
-
-
5
3
-
-
3
2
-
10
-
-
1
-
-
-
1
-
6
1
2
-
1
-
1
-
-
1
-
-
-
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-
1
1
-
-
-
-
5
-
3
-
-
3
2
-
-
-
1
-
-
1
-
6
1
2
-
1
-
1
-
-
-
-
-
-
-
-
-
286417
Rumsby
Identification and expression ...
Homo sapiens
Biochim. Biophys. Acta
1446
383-388
1999
-
-
1
-
-
-
-
-
-
-
2
-
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1
-
-
-
-
-
1
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1
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1
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-
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2
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-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286416
Giafi
Kinetic analysis and tissue di ...
Homo sapiens
Ann. Clin. Biochem.
35
104-109
1998
-
-
-
-
-
-
-
1
-
-
-
-
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2
-
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2
1
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1
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1
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2
1
-
-
-
-
-
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-
1
-
-
-
-
-
-
-
-
-
286418
Givan
The enzymic reduction of glyox ...
Hordeum vulgare, Nicotiana tabacum, Petroselinum crispum, Pisum sativum, Spinacia oleracea, Zea mays
Plant Physiol.
100
552-556
1992
-
-
-
-
1
-
6
5
9
-
5
7
-
10
-
-
1
-
-
5
2
-
12
2
-
-
-
-
-
2
-
6
-
-
-
-
-
-
6
-
1
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-
6
-
5
9
-
5
7
-
-
-
1
-
5
2
-
12
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
286419
Betsche
Identification and characteria ...
Cyanophora paradoxa, Pisum sativum
Plant Physiol.
98
887-893
1992
-
-
-
-
-
-
4
-
5
-
-
6
-
6
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2
1
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1
10
-
8
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2
1
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4
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4
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4
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5
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6
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2
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1
10
-
8
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
286409
Yokota
Occurrence and subcellular dis ...
Euglena gracilis
Agric. Biol. Chem.
45
15-22
1981
-
-
-
-
-
-
-
-
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1
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1
1
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2
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2
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-
1
1
-
-
-
-
-
-
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-
-
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-
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-
286408
Hullin
Glyoxylate reductase, two form ...
Pseudomonas fluorescens
Methods Enzymol.
41B
343-348
1975
-
-
-
-
-
-
2
-
-
-
1
-
-
2
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1
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3
-
2
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1
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2
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2
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2
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1
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1
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3
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2
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-
1
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-
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-
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-
286414
Bergmeyer
-
Glyoxylate reductase ...
Spinacia oleracea
Methods Enzym. Anal. , 3rd Ed. (Bergmeyer, H. U. , ed. )
2
126-328
1974
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-
-
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1
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1
1
3
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1
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1
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1
1
3
-
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-
286410
Suzuki
Studies on peroxisomes. IV. in ...
Rattus norvegicus
J. Biochem.
73
1033-1038
1973
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-
-
-
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1
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1
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1
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2
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2
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1
-
-
1
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-
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-
-
-
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-
-
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-
-
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-
286413
Cerff
Glyceraldehyde 3-phosphate deh ...
Sinapis alba
Plant Physiol.
51
76-81
1973
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3
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3
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1
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2
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2
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3
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1
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-
-
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-
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-
-
-
286411
Zelitch
-
Glycolic acid oxidase and glyo ...
Nicotiana tabacum, Spinacia oleracea
Methods Enzymol.
1
528-535
1955
-
-
-
-
-
-
3
-
-
-
-
-
-
2
-
-
1
-
-
2
2
-
2
-
-
-
-
-
1
-
-
4
-
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-
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-
4
-
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3
-
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-
1
-
2
2
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286412
Zelitch
The isolation and action of cr ...
Nicotiana tabacum
J. Biol. Chem.
216
553-575
1955
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-
-
1
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-
-
-
-
-
-
-
-
2
-
-
1
-
-
1
1
-
1
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1
1
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2
-
-
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-
2
1
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-
-
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-
1
-
1
1
-
1
-
-
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-
1
1
-
-
-
-
-
-
-
-
286415
Zelitch
Oxidation and reduction of gly ...
Spinacia oleracea
J. Biol. Chem.
201
719-726
1953
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