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Literature summary for 1.1.1.26 extracted from

  • Hoover, G.J.; Van Cauwenberghe, O.R.; Breitkreuz, K.E.; Clark, S.M.; Merrill, A.R.; Shelp, B.J.
    Characteristics of an Arabidopsis glyoxylate reductase: general biochemical properties and substrate specificity for the recombinant protein, and developmental expression and implications for glyoxylate and succinic semialdehyde metabolism in planta (2007), Can. J. Bot., 85, 883-895.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His6-tagged enzyme in Escherichia coli srain BL21 Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Arabidopsis thaliana
0.0026
-
NADPH pH 7.8, 22°C, recombinant His6-tagged enzyme Arabidopsis thaliana
0.0045
-
glyoxylate pH 7.8, 22°C, recombinant His6-tagged enzyme Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21 by PEG precipitation, nickel affinity and anion exchange chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Arabidopsis thaliana
-
additional information tissue-specific expression, overview Arabidopsis thaliana
-
root low activity Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.28
-
purified recombinant His6-tagged enzyme Arabidopsis thaliana

Storage Stability

Storage Stability Organism
-80°C, partially purified recombinant His6-tagged enzyme in an ammonium sulfate pellet, 6 months, stable Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glyoxylate + NADPH a recombinant gamma hydroxybutyrate dehydrogenase, EC 1.1.1.61, exhibits high glyoxylate reductase activity with a 250fold higher preference for glyoxylate than with succinic semialdehyde, via an essentially irreversible, NADPH-based mechanism, overview Arabidopsis thaliana glycolate + NADP+
-
ir
additional information the enzyme prefers succinic semialdehyde 100fold to formaldehyde, acetaldehyde, butyraldehyde, 2-carboxybenzaldehyde, glyoxal, methylglyoxal, phenylglyoxal, and phenylglyoxylate Arabidopsis thaliana ?
-
?

Subunits

Subunits Comment Organism
? x * 33000-35000, recombinant His6-tagged enzyme, SDS-PAGE Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
recombinant His6-tagged enzyme Arabidopsis thaliana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 57.5
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.1
-
NADPH pH 7.8, 22°C, recombinant His6-tagged enzyme Arabidopsis thaliana
13
-
glyoxylate pH 7.8, 22°C, recombinant His6-tagged enzyme Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
recombinant His6-tagged enzyme Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
4 12
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Arabidopsis thaliana