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Literature summary for 1.1.1.26 extracted from

  • Fauvart, M.; Braeken, K.; Daniels, R.; Vos, K.; Ndayizeye, M.; Noben, J.; Robben, J.; Vanderleyden, J.; Michiels, J.
    Identification of a novel glyoxylate reductase supports phylogeny-based enzymatic substrate specificity prediction (2007), Biochim. Biophys. Acta, 1774, 1092-1098.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene gxrA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21 Rhizobium etli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
phenylpyruvate pH 7.0, 37°C Rhizobium etli
2.5
-
glyoxylate pH 7.0, 37°C Rhizobium etli
6.8
-
Hydroxypyruvate pH 7.0, 37°C Rhizobium etli
40
-
2-oxobutyrate pH 7.0, 37°C Rhizobium etli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
2 * 37000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 38420, recombinant His-tagged enzyme, MALDI-TOF mass spectrometry Rhizobium etli
38420
-
2 * 37000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 38420, recombinant His-tagged enzyme, MALDI-TOF mass spectrometry Rhizobium etli
77200
-
recombinant His-tagged enzyme, gel filtration Rhizobium etli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glyoxylate + NADH Rhizobium etli GxrA glycolate + NAD+
-
ir
glyoxylate + NADH Rhizobium etli CFN 42 GxrA glycolate + NAD+
-
ir
additional information Rhizobium etli in the endosymbiont, a 3-phosphoglycerate dehydrogenase exhibits glyoxylate/hydroxypyruvate reductase activity, substrate specificity, overview ?
-
?
additional information Rhizobium etli CFN 42 in the endosymbiont, a 3-phosphoglycerate dehydrogenase exhibits glyoxylate/hydroxypyruvate reductase activity, substrate specificity, overview ?
-
?
phenylpyruvate + NADH Rhizobium etli GxrA phenyllactate + NAD+
-
ir
phenylpyruvate + NADH Rhizobium etli CFN 42 GxrA phenyllactate + NAD+
-
ir

Organism

Organism UniProt Comment Textmining
Rhizobium etli
-
strain CFN42, gene gxrA
-
Rhizobium etli CFN 42
-
strain CFN42, gene gxrA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration Rhizobium etli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutyrate + NADH GxrA Rhizobium etli isovalerate + NAD+
-
?
glyoxylate + NADH GxrA Rhizobium etli glycolate + NAD+
-
ir
glyoxylate + NADH GxrA Rhizobium etli CFN 42 glycolate + NAD+
-
ir
hydroxypyruvate + NADH GxrA Rhizobium etli D-glycerate + NAD+
-
?
hydroxypyruvate + NADH GxrA Rhizobium etli CFN 42 D-glycerate + NAD+
-
?
additional information in the endosymbiont, a 3-phosphoglycerate dehydrogenase exhibits glyoxylate/hydroxypyruvate reductase activity, substrate specificity, overview Rhizobium etli ?
-
?
additional information no activity of GxrA with 2-oxoglutarate, 2-oxo-D-gluconate, and indole in the reductive direction, as well as with glycolate, D-phenyllactate, and D-gluconate in the oxidative direction Rhizobium etli ?
-
?
additional information in the endosymbiont, a 3-phosphoglycerate dehydrogenase exhibits glyoxylate/hydroxypyruvate reductase activity, substrate specificity, overview Rhizobium etli CFN 42 ?
-
?
additional information no activity of GxrA with 2-oxoglutarate, 2-oxo-D-gluconate, and indole in the reductive direction, as well as with glycolate, D-phenyllactate, and D-gluconate in the oxidative direction Rhizobium etli CFN 42 ?
-
?
phenylpyruvate + NADH GxrA Rhizobium etli phenyllactate + NAD+
-
ir
phenylpyruvate + NADH GxrA Rhizobium etli CFN 42 phenyllactate + NAD+
-
ir

Subunits

Subunits Comment Organism
dimer 2 * 37000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 38420, recombinant His-tagged enzyme, MALDI-TOF mass spectrometry Rhizobium etli

Synonyms

Synonyms Comment Organism
GHPR
-
Rhizobium etli
glyoxylate/hydroxypyruvate reductase
-
Rhizobium etli
GxrA a 3-phosphoglycerate dehydrogenase exhibiting glyoxylate/hydroxypyruvate reductase activity Rhizobium etli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rhizobium etli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.25
-
Hydroxypyruvate pH 7.0, 37°C Rhizobium etli
0.37
-
phenylpyruvate pH 7.0, 37°C Rhizobium etli
0.5
-
2-oxobutyrate pH 7.0, 37°C Rhizobium etli
1.35
-
glyoxylate pH 7.0, 37°C Rhizobium etli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
with substrate phenylpyruvate Rhizobium etli

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.5
-
Rhizobium etli

Cofactor

Cofactor Comment Organism Structure
NADH is not replacable by NADPH with GxrA Rhizobium etli