BRENDA - Enzyme Database show
show all sequences of 1.1.1.26

Identification of a novel glyoxylate reductase supports phylogeny-based enzymatic substrate specificity prediction

Fauvart, M.; Braeken, K.; Daniels, R.; Vos, K.; Ndayizeye, M.; Noben, J.; Robben, J.; Vanderleyden, J.; Michiels, J.; Biochim. Biophys. Acta 1774, 1092-1098 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene gxrA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21
Rhizobium etli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.8
-
phenylpyruvate
pH 7.0, 37°C
Rhizobium etli
2.5
-
glyoxylate
pH 7.0, 37°C
Rhizobium etli
6.8
-
Hydroxypyruvate
pH 7.0, 37°C
Rhizobium etli
40
-
2-oxobutyrate
pH 7.0, 37°C
Rhizobium etli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
2 * 37000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 38420, recombinant His-tagged enzyme, MALDI-TOF mass spectrometry
Rhizobium etli
38420
-
2 * 37000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 38420, recombinant His-tagged enzyme, MALDI-TOF mass spectrometry
Rhizobium etli
77200
-
recombinant His-tagged enzyme, gel filtration
Rhizobium etli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glyoxylate + NADH
Rhizobium etli
GxrA
glycolate + NAD+
-
-
ir
glyoxylate + NADH
Rhizobium etli CFN42
GxrA
glycolate + NAD+
-
-
ir
additional information
Rhizobium etli
in the endosymbiont, a 3-phosphoglycerate dehydrogenase exhibits glyoxylate/hydroxypyruvate reductase activity, substrate specificity, overview
?
-
-
-
additional information
Rhizobium etli CFN42
in the endosymbiont, a 3-phosphoglycerate dehydrogenase exhibits glyoxylate/hydroxypyruvate reductase activity, substrate specificity, overview
?
-
-
-
phenylpyruvate + NADH
Rhizobium etli
GxrA
phenyllactate + NAD+
-
-
ir
phenylpyruvate + NADH
Rhizobium etli CFN42
GxrA
phenyllactate + NAD+
-
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhizobium etli
-
strain CFN42, gene gxrA
-
Rhizobium etli CFN42
-
strain CFN42, gene gxrA
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration
Rhizobium etli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxobutyrate + NADH
GxrA
685349
Rhizobium etli
isovalerate + NAD+
-
-
-
?
glyoxylate + NADH
GxrA
685349
Rhizobium etli
glycolate + NAD+
-
-
-
ir
glyoxylate + NADH
GxrA
685349
Rhizobium etli CFN42
glycolate + NAD+
-
-
-
ir
hydroxypyruvate + NADH
GxrA
685349
Rhizobium etli
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADH
GxrA
685349
Rhizobium etli CFN42
D-glycerate + NAD+
-
-
-
?
additional information
in the endosymbiont, a 3-phosphoglycerate dehydrogenase exhibits glyoxylate/hydroxypyruvate reductase activity, substrate specificity, overview
685349
Rhizobium etli
?
-
-
-
-
additional information
no activity of GxrA with 2-oxoglutarate, 2-oxo-D-gluconate, and indole in the reductive direction, as well as with glycolate, D-phenyllactate, and D-gluconate in the oxidative direction
685349
Rhizobium etli
?
-
-
-
-
additional information
in the endosymbiont, a 3-phosphoglycerate dehydrogenase exhibits glyoxylate/hydroxypyruvate reductase activity, substrate specificity, overview
685349
Rhizobium etli CFN42
?
-
-
-
-
additional information
no activity of GxrA with 2-oxoglutarate, 2-oxo-D-gluconate, and indole in the reductive direction, as well as with glycolate, D-phenyllactate, and D-gluconate in the oxidative direction
685349
Rhizobium etli CFN42
?
-
-
-
-
phenylpyruvate + NADH
GxrA
685349
Rhizobium etli
phenyllactate + NAD+
-
-
-
ir
phenylpyruvate + NADH
GxrA
685349
Rhizobium etli CFN42
phenyllactate + NAD+
-
-
-
ir
Subunits
Subunits
Commentary
Organism
dimer
2 * 37000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 38420, recombinant His-tagged enzyme, MALDI-TOF mass spectrometry
Rhizobium etli
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rhizobium etli
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.25
-
Hydroxypyruvate
pH 7.0, 37°C
Rhizobium etli
0.37
-
phenylpyruvate
pH 7.0, 37°C
Rhizobium etli
0.5
-
2-oxobutyrate
pH 7.0, 37°C
Rhizobium etli
1.35
-
glyoxylate
pH 7.0, 37°C
Rhizobium etli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
with substrate phenylpyruvate
Rhizobium etli
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
9.5
-
Rhizobium etli
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
is not replacable by NADPH with GxrA
Rhizobium etli
Cloned(Commentary) (protein specific)
Commentary
Organism
gene gxrA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21
Rhizobium etli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
is not replacable by NADPH with GxrA
Rhizobium etli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.8
-
phenylpyruvate
pH 7.0, 37°C
Rhizobium etli
2.5
-
glyoxylate
pH 7.0, 37°C
Rhizobium etli
6.8
-
Hydroxypyruvate
pH 7.0, 37°C
Rhizobium etli
40
-
2-oxobutyrate
pH 7.0, 37°C
Rhizobium etli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
2 * 37000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 38420, recombinant His-tagged enzyme, MALDI-TOF mass spectrometry
Rhizobium etli
38420
-
2 * 37000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 38420, recombinant His-tagged enzyme, MALDI-TOF mass spectrometry
Rhizobium etli
77200
-
recombinant His-tagged enzyme, gel filtration
Rhizobium etli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glyoxylate + NADH
Rhizobium etli
GxrA
glycolate + NAD+
-
-
ir
glyoxylate + NADH
Rhizobium etli CFN42
GxrA
glycolate + NAD+
-
-
ir
additional information
Rhizobium etli
in the endosymbiont, a 3-phosphoglycerate dehydrogenase exhibits glyoxylate/hydroxypyruvate reductase activity, substrate specificity, overview
?
-
-
-
additional information
Rhizobium etli CFN42
in the endosymbiont, a 3-phosphoglycerate dehydrogenase exhibits glyoxylate/hydroxypyruvate reductase activity, substrate specificity, overview
?
-
-
-
phenylpyruvate + NADH
Rhizobium etli
GxrA
phenyllactate + NAD+
-
-
ir
phenylpyruvate + NADH
Rhizobium etli CFN42
GxrA
phenyllactate + NAD+
-
-
ir
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration
Rhizobium etli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxobutyrate + NADH
GxrA
685349
Rhizobium etli
isovalerate + NAD+
-
-
-
?
glyoxylate + NADH
GxrA
685349
Rhizobium etli
glycolate + NAD+
-
-
-
ir
glyoxylate + NADH
GxrA
685349
Rhizobium etli CFN42
glycolate + NAD+
-
-
-
ir
hydroxypyruvate + NADH
GxrA
685349
Rhizobium etli
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADH
GxrA
685349
Rhizobium etli CFN42
D-glycerate + NAD+
-
-
-
?
additional information
in the endosymbiont, a 3-phosphoglycerate dehydrogenase exhibits glyoxylate/hydroxypyruvate reductase activity, substrate specificity, overview
685349
Rhizobium etli
?
-
-
-
-
additional information
no activity of GxrA with 2-oxoglutarate, 2-oxo-D-gluconate, and indole in the reductive direction, as well as with glycolate, D-phenyllactate, and D-gluconate in the oxidative direction
685349
Rhizobium etli
?
-
-
-
-
additional information
in the endosymbiont, a 3-phosphoglycerate dehydrogenase exhibits glyoxylate/hydroxypyruvate reductase activity, substrate specificity, overview
685349
Rhizobium etli CFN42
?
-
-
-
-
additional information
no activity of GxrA with 2-oxoglutarate, 2-oxo-D-gluconate, and indole in the reductive direction, as well as with glycolate, D-phenyllactate, and D-gluconate in the oxidative direction
685349
Rhizobium etli CFN42
?
-
-
-
-
phenylpyruvate + NADH
GxrA
685349
Rhizobium etli
phenyllactate + NAD+
-
-
-
ir
phenylpyruvate + NADH
GxrA
685349
Rhizobium etli CFN42
phenyllactate + NAD+
-
-
-
ir
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 37000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 38420, recombinant His-tagged enzyme, MALDI-TOF mass spectrometry
Rhizobium etli
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rhizobium etli
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.25
-
Hydroxypyruvate
pH 7.0, 37°C
Rhizobium etli
0.37
-
phenylpyruvate
pH 7.0, 37°C
Rhizobium etli
0.5
-
2-oxobutyrate
pH 7.0, 37°C
Rhizobium etli
1.35
-
glyoxylate
pH 7.0, 37°C
Rhizobium etli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
with substrate phenylpyruvate
Rhizobium etli
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
9.5
-
Rhizobium etli
Other publictions for EC 1.1.1.26
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722899
Ching
Glyoxylate reductase isoform 1 ...
Arabidopsis thaliana
J. Integr. Plant Biol.
54
152-168
2012
-
-
1
-
-
-
-
-
4
-
-
2
-
3
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
4
-
-
-
-
-
-
-
4
-
-
2
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
715548
Nunn
Metabolism of pentose sugars i ...
Sulfolobus solfataricus, Sulfolobus solfataricus P2
J. Biol. Chem.
285
33701-33709
2010
-
-
1
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
696125
Allan
Role of plant glyoxylate reduc ...
Arabidopsis thaliana
Biochem. J.
423
15-22
2009
-
-
-
-
1
-
-
-
2
-
-
3
-
3
-
-
-
-
-
3
-
-
4
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
2
-
-
3
-
-
-
-
-
3
-
-
4
1
-
-
-
-
-
-
-
-
1
2
2
1
-
-
684838
Shinoda
A highly specific glyoxylate r ...
Paracoccus sp.
Biochem. Biophys. Res. Commun.
355
782-787
2007
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
1
-
-
1
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
685349
Fauvart
Identification of a novel glyo ...
Rhizobium etli, Rhizobium etli CFN42
Biochim. Biophys. Acta
1774
1092-1098
2007
-
-
1
-
-
-
-
4
-
-
3
6
-
6
-
-
1
-
-
-
-
-
11
1
1
-
-
4
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
4
-
-
3
6
-
-
-
1
-
-
-
-
11
1
1
-
-
4
1
1
-
-
-
-
-
-
-
-
685901
Hoover
Characteristics of an Arabidop ...
Arabidopsis thaliana
Can. J. Bot.
85
883-895
2007
-
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
1
-
-
3
1
1
2
1
1
1
-
2
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
3
1
1
2
1
1
1
-
2
1
1
-
-
-
-
-
-
-
-
655702
Cregeen
Molecular analysis of the glyo ...
Homo sapiens
Hum. Mutat.
22
497
2003
-
1
-
-
3
-
-
-
-
-
-
-
-
2
-
-
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-
1
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1
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-
3
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-
-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727414
Ohshima
A novel hyperthermophilic arch ...
Thermococcus litoralis, Thermococcus litoralis DSM 5473
Eur. J. Biochem.
268
4740-4747
2001
-
-
1
-
-
-
5
3
-
-
3
2
-
10
-
-
1
-
-
-
1
-
6
1
2
-
1
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
5
-
3
-
-
3
2
-
-
-
1
-
-
1
-
6
1
2
-
1
-
1
-
-
-
-
-
-
-
-
-
286417
Rumsby
Identification and expression ...
Homo sapiens
Biochim. Biophys. Acta
1446
383-388
1999
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286416
Giafi
Kinetic analysis and tissue di ...
Homo sapiens
Ann. Clin. Biochem.
35
104-109
1998
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286418
Givan
The enzymic reduction of glyox ...
Hordeum vulgare, Nicotiana tabacum, Petroselinum crispum, Pisum sativum, Spinacia oleracea, Zea mays
Plant Physiol.
100
552-556
1992
-
-
-
-
1
-
6
5
9
-
5
7
-
10
-
-
1
-
-
5
2
-
12
2
-
-
-
-
-
2
-
6
-
-
-
-
-
-
6
-
1
-
-
6
-
5
9
-
5
7
-
-
-
1
-
5
2
-
12
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
286419
Betsche
Identification and characteria ...
Cyanophora paradoxa, Pisum sativum
Plant Physiol.
98
887-893
1992
-
-
-
-
-
-
4
-
5
-
-
6
-
6
-
-
2
1
-
1
10
-
8
-
-
-
-
-
2
1
-
4
-
-
-
-
-
-
4
-
-
-
-
4
-
-
5
-
-
6
-
-
-
2
-
1
10
-
8
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
286409
Yokota
Occurrence and subcellular dis ...
Euglena gracilis
Agric. Biol. Chem.
45
15-22
1981
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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286408
Hullin
Glyoxylate reductase, two form ...
Pseudomonas fluorescens
Methods Enzymol.
41B
343-348
1975
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2
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1
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286414
Bergmeyer
-
Glyoxylate reductase ...
Spinacia oleracea
Methods Enzym. Anal. , 3rd Ed. (Bergmeyer, H. U. , ed. )
2
126-328
1974
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1
1
3
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286410
Suzuki
Studies on peroxisomes. IV. in ...
Rattus norvegicus
J. Biochem.
73
1033-1038
1973
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286413
Cerff
Glyceraldehyde 3-phosphate deh ...
Sinapis alba
Plant Physiol.
51
76-81
1973
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286411
Zelitch
-
Glycolic acid oxidase and glyo ...
Nicotiana tabacum, Spinacia oleracea
Methods Enzymol.
1
528-535
1955
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3
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286412
Zelitch
The isolation and action of cr ...
Nicotiana tabacum
J. Biol. Chem.
216
553-575
1955
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1
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286415
Zelitch
Oxidation and reduction of gly ...
Spinacia oleracea
J. Biol. Chem.
201
719-726
1953
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