Cloned (Comment) | Organism |
---|---|
gene gxrA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21 | Rhizobium etli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
phenylpyruvate | pH 7.0, 37°C | Rhizobium etli | |
2.5 | - |
glyoxylate | pH 7.0, 37°C | Rhizobium etli | |
6.8 | - |
Hydroxypyruvate | pH 7.0, 37°C | Rhizobium etli | |
40 | - |
2-oxobutyrate | pH 7.0, 37°C | Rhizobium etli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
2 * 37000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 38420, recombinant His-tagged enzyme, MALDI-TOF mass spectrometry | Rhizobium etli |
38420 | - |
2 * 37000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 38420, recombinant His-tagged enzyme, MALDI-TOF mass spectrometry | Rhizobium etli |
77200 | - |
recombinant His-tagged enzyme, gel filtration | Rhizobium etli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + NADH | Rhizobium etli | GxrA | glycolate + NAD+ | - |
ir | |
glyoxylate + NADH | Rhizobium etli CFN 42 | GxrA | glycolate + NAD+ | - |
ir | |
additional information | Rhizobium etli | in the endosymbiont, a 3-phosphoglycerate dehydrogenase exhibits glyoxylate/hydroxypyruvate reductase activity, substrate specificity, overview | ? | - |
? | |
additional information | Rhizobium etli CFN 42 | in the endosymbiont, a 3-phosphoglycerate dehydrogenase exhibits glyoxylate/hydroxypyruvate reductase activity, substrate specificity, overview | ? | - |
? | |
phenylpyruvate + NADH | Rhizobium etli | GxrA | phenyllactate + NAD+ | - |
ir | |
phenylpyruvate + NADH | Rhizobium etli CFN 42 | GxrA | phenyllactate + NAD+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizobium etli | - |
strain CFN42, gene gxrA | - |
Rhizobium etli CFN 42 | - |
strain CFN42, gene gxrA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration | Rhizobium etli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutyrate + NADH | GxrA | Rhizobium etli | isovalerate + NAD+ | - |
? | |
glyoxylate + NADH | GxrA | Rhizobium etli | glycolate + NAD+ | - |
ir | |
glyoxylate + NADH | GxrA | Rhizobium etli CFN 42 | glycolate + NAD+ | - |
ir | |
hydroxypyruvate + NADH | GxrA | Rhizobium etli | D-glycerate + NAD+ | - |
? | |
hydroxypyruvate + NADH | GxrA | Rhizobium etli CFN 42 | D-glycerate + NAD+ | - |
? | |
additional information | in the endosymbiont, a 3-phosphoglycerate dehydrogenase exhibits glyoxylate/hydroxypyruvate reductase activity, substrate specificity, overview | Rhizobium etli | ? | - |
? | |
additional information | no activity of GxrA with 2-oxoglutarate, 2-oxo-D-gluconate, and indole in the reductive direction, as well as with glycolate, D-phenyllactate, and D-gluconate in the oxidative direction | Rhizobium etli | ? | - |
? | |
additional information | in the endosymbiont, a 3-phosphoglycerate dehydrogenase exhibits glyoxylate/hydroxypyruvate reductase activity, substrate specificity, overview | Rhizobium etli CFN 42 | ? | - |
? | |
additional information | no activity of GxrA with 2-oxoglutarate, 2-oxo-D-gluconate, and indole in the reductive direction, as well as with glycolate, D-phenyllactate, and D-gluconate in the oxidative direction | Rhizobium etli CFN 42 | ? | - |
? | |
phenylpyruvate + NADH | GxrA | Rhizobium etli | phenyllactate + NAD+ | - |
ir | |
phenylpyruvate + NADH | GxrA | Rhizobium etli CFN 42 | phenyllactate + NAD+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 38420, recombinant His-tagged enzyme, MALDI-TOF mass spectrometry | Rhizobium etli |
Synonyms | Comment | Organism |
---|---|---|
GHPR | - |
Rhizobium etli |
glyoxylate/hydroxypyruvate reductase | - |
Rhizobium etli |
GxrA | a 3-phosphoglycerate dehydrogenase exhibiting glyoxylate/hydroxypyruvate reductase activity | Rhizobium etli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rhizobium etli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
Hydroxypyruvate | pH 7.0, 37°C | Rhizobium etli | |
0.37 | - |
phenylpyruvate | pH 7.0, 37°C | Rhizobium etli | |
0.5 | - |
2-oxobutyrate | pH 7.0, 37°C | Rhizobium etli | |
1.35 | - |
glyoxylate | pH 7.0, 37°C | Rhizobium etli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
with substrate phenylpyruvate | Rhizobium etli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | - |
Rhizobium etli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | is not replacable by NADPH with GxrA | Rhizobium etli |