Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant lacking cytosolic hydroxypyruvate-metabolizing activity (HPR-1) | Hordeum vulgare |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Acetohydroxamate | inhibits only the cytosolic enzyme type with preference for glyoxylate as substrate | Pisum sativum | |
Acetohydroxamate | inhibits only the cytosolic enzyme type with preference for glyoxylate as substrate | Spinacia oleracea | |
oxalate | inhibits only the cytosolic enzyme type with preference for hydroxypyruvate as substrate | Pisum sativum | |
oxalate | inhibits only the cytosolic enzyme type with preference for hydroxypyruvate as substrate | Spinacia oleracea | |
Tartronate | inhibits only the cytosolic enzyme type with preference for hydroxypyruvate as substrate | Pisum sativum | |
Tartronate | inhibits only the cytosolic enzyme type with preference for hydroxypyruvate as substrate | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | 0.11 | glyoxylate | NADPH, cytosolic enzyme | Spinacia oleracea | |
0.07 | 0.12 | Hydroxypyruvate | NADH, peroxisomal enzyme type with preference for hydroxypyruvate and NADH as substrates | Spinacia oleracea | |
0.8 | - |
Hydroxypyruvate | NADPH, cytosolic enzyme type with preference for hydroxypyruvate and NADPH as substrates | Spinacia oleracea | |
1.1 | - |
glyoxylate | NADPH, cytosolic enzyme type with preference for hydroxypyruvate as substrate | Spinacia oleracea | |
10 | 25 | glyoxylate | NADH, peroxisomal enzyme type with preference for hydroxypyruvate and NADH as substrates | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | utilizes NADH and NADPH equally | Spinacia oleracea | 9507 | - |
chloroplast | utilizes NADH and NADPH equally | Pisum sativum | 9507 | - |
cytosol | - |
Hordeum vulgare | 5829 | - |
cytosol | NADP+/NADPH preferring type | Spinacia oleracea | 5829 | - |
cytosol | NADP+/NADPH preferring type | Pisum sativum | 5829 | - |
peroxisome | - |
Hordeum vulgare | 5777 | - |
peroxisome | NAD+/NADH preferring type | Spinacia oleracea | 5777 | - |
peroxisome | NAD+/NADH preferring type | Pisum sativum | 5777 | - |
peroxisome | NAD+/NADH preferring type | Petroselinum crispum | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
4 * 33000, cytosolic enzyme | Spinacia oleracea |
38000 | - |
2 * 38000, cytosolic enzyme with preference for hydroxypyruvate as substrate | Spinacia oleracea |
70000 | - |
cytosolic enzyme with preference for hydroxypyruvate as substrate | Spinacia oleracea |
90000 | - |
peroxisomal enzyme with preference for hydroxypyruvate as substrate | Spinacia oleracea |
125000 | - |
cytosolic enzyme | Spinacia oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycolate + NAD+ | Spinacia oleracea | - |
glyoxylate + NADH | - |
r | |
glycolate + NAD+ | Pisum sativum | - |
glyoxylate + NADH | - |
r | |
glyoxylate + NADH | Spinacia oleracea | - |
glycolate + NAD+ | - |
r | |
glyoxylate + NADH | Pisum sativum | - |
glycolate + NAD+ | - |
r | |
hydroxypyruvate + NADH | Pisum sativum | - |
D-glycerate + NAD+ | - |
r | |
hydroxypyruvate + NADH | Spinacia oleracea | located exclusively in the peroxisomes, prefers NAD+/NADH | D-glycerate + NAD+ | - |
r | |
hydroxypyruvate + NADH | Petroselinum crispum | located exclusively in the peroxisomes, prefers NAD+/NADH | D-glycerate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
barley | - |
Nicotiana tabacum | - |
tobacco | - |
Petroselinum crispum | - |
parsley | - |
Pisum sativum | - |
- |
- |
Spinacia oleracea | - |
spinach | - |
Zea mays | - |
maize | - |
Purification (Comment) | Organism |
---|---|
- |
Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
leaf | - |
Pisum sativum | - |
leaf | - |
Zea mays | - |
leaf | - |
Nicotiana tabacum | - |
leaf | mutant without cytosolic hydroxypyruvate-metabolizing activity | Hordeum vulgare | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.05 | 0.23 | NADPH, cytosolic enzyme with preference for hydroxypyruvate as substrate | Spinacia oleracea |
0.3 | 1.5 | NADH, peroxisomal enzyme type with preference for hydroxypyruvate and NADH as substrates | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycolate + NAD+ | - |
Spinacia oleracea | glyoxylate + NADH | - |
r | |
glycolate + NAD+ | - |
Pisum sativum | glyoxylate + NADH | - |
r | |
glyoxylate + NADH | - |
Spinacia oleracea | glycolate + NAD+ | - |
r | |
glyoxylate + NADH | - |
Pisum sativum | glycolate + NAD+ | - |
r | |
glyoxylate + NADH | equilibrium is very far in direction of glycolate formation | Spinacia oleracea | glycolate + NAD+ | - |
r | |
glyoxylate + NADH | equilibrium is very far in direction of glycolate formation | Pisum sativum | glycolate + NAD+ | - |
r | |
glyoxylate + NADH | equilibrium is very far in direction of glycolate formation | Nicotiana tabacum | glycolate + NAD+ | - |
r | |
hydroxypyruvate + NADH | - |
Spinacia oleracea | D-glycerate + NAD+ | - |
r | |
hydroxypyruvate + NADH | - |
Pisum sativum | D-glycerate + NAD+ | - |
r | |
hydroxypyruvate + NADH | - |
Petroselinum crispum | D-glycerate + NAD+ | - |
r | |
hydroxypyruvate + NADH | located exclusively in the peroxisomes, prefers NAD+/NADH | Spinacia oleracea | D-glycerate + NAD+ | - |
r | |
hydroxypyruvate + NADH | located exclusively in the peroxisomes, prefers NAD+/NADH | Petroselinum crispum | D-glycerate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38000, cytosolic enzyme with preference for hydroxypyruvate as substrate | Spinacia oleracea |
tetramer | 4 * 33000, cytosolic enzyme | Spinacia oleracea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.8 | 7.4 | the optimal pH for hydroxypyruvate reduction is more acidic than that for glyoxylate reduction | Spinacia oleracea |
5.8 | 7.4 | the optimal pH for hydroxypyruvate reduction is more acidic than that for glyoxylate reduction | Pisum sativum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pisum sativum | |
NAD+ | - |
Nicotiana tabacum | |
NAD+ | enzyme of peroxisomes and chloroplasts prefer NAD+, enzymes of the cytosol prefer NADP+ | Spinacia oleracea | |
NADH | - |
Pisum sativum | |
NADH | - |
Nicotiana tabacum | |
NADH | enzyme of peroxisomes and chloroplasts prefer NADH, enzymes of the cytosol prefer NADPH | Spinacia oleracea |