BRENDA - Enzyme Database show
show all sequences of 1.1.1.255

Characterization of NAD-dependent mannitol dehydrogenase from celery as affected by ions, chelators, reducing agents and metabolites

Stoop, J.M.H.; Williamson, J.D.; Conkling, M.A.; MacKay, J.J.; Pharr, D.M.; Plant Sci. 131, 43-51 (1998)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
dose dependent decrease in enzyme activity
Apium graveolens
1,4-dithioerythritol
1.09 mM, 50% inhibition, partially reversed by diamide
Apium graveolens
1,4-dithiothreitol
0.57 mM, 50% inhibition, reversed by diamide
Apium graveolens
2-mercaptoethanol
30 mM, 50% inhibition, partially reversed by diamide
Apium graveolens
ADP
Ki: 0.24 mM at pH 7.0
Apium graveolens
AMP
Ki: 0.50-0.80 mM at pH 9.5, Ki = 0.64 mM at pH 7.0
Apium graveolens
ATP
Ki: 1.10 mM at pH 7.0
Apium graveolens
additional information
EDTA and EGTA did not affect enzyme activity
Apium graveolens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00127
-
NADH
-
Apium graveolens
0.14
-
NAD+
at pH 9.5
Apium graveolens
0.23
-
NAD+
at pH 7.0
Apium graveolens
64
-
D-mannitol
at pH 9.5
Apium graveolens
400
-
D-mannitol
at pH 7.0
Apium graveolens
745
-
D-mannose
-
Apium graveolens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Apium graveolens
-
celery; L. var. dulce Mill. Pers.
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.9
-
at pH 9.5
Apium graveolens
2.2
-
at pH 7.0
Apium graveolens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-mannitol + NAD+
-
286404
Apium graveolens
D-mannose + NADH + H+
-
286404
Apium graveolens
r
additional information
no cinnamyl alcohol dehydrogenase activity
286404
Apium graveolens
?
-
-
-
-
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
-
D-mannose reduction
Apium graveolens
9.5
-
D-mannitol oxidation
Apium graveolens
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Apium graveolens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Apium graveolens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
dose dependent decrease in enzyme activity
Apium graveolens
1,4-dithioerythritol
1.09 mM, 50% inhibition, partially reversed by diamide
Apium graveolens
1,4-dithiothreitol
0.57 mM, 50% inhibition, reversed by diamide
Apium graveolens
2-mercaptoethanol
30 mM, 50% inhibition, partially reversed by diamide
Apium graveolens
ADP
Ki: 0.24 mM at pH 7.0
Apium graveolens
AMP
Ki: 0.50-0.80 mM at pH 9.5, Ki = 0.64 mM at pH 7.0
Apium graveolens
ATP
Ki: 1.10 mM at pH 7.0
Apium graveolens
additional information
EDTA and EGTA did not affect enzyme activity
Apium graveolens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00127
-
NADH
-
Apium graveolens
0.14
-
NAD+
at pH 9.5
Apium graveolens
0.23
-
NAD+
at pH 7.0
Apium graveolens
64
-
D-mannitol
at pH 9.5
Apium graveolens
400
-
D-mannitol
at pH 7.0
Apium graveolens
745
-
D-mannose
-
Apium graveolens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.9
-
at pH 9.5
Apium graveolens
2.2
-
at pH 7.0
Apium graveolens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-mannitol + NAD+
-
286404
Apium graveolens
D-mannose + NADH + H+
-
286404
Apium graveolens
r
additional information
no cinnamyl alcohol dehydrogenase activity
286404
Apium graveolens
?
-
-
-
-
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
-
D-mannose reduction
Apium graveolens
9.5
-
D-mannitol oxidation
Apium graveolens
Other publictions for EC 1.1.1.255
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
700868
Cheng
Salicylic acid stimulates secr ...
Apium graveolens
Planta
230
1093-1103
2009
1
-
1
-
-
-
-
-
1
-
1
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686483
Mustorp
Detection of celery (Apium gra ...
Apium graveolens
Eur. Food Res. Technol.
226
771-778
2008
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656997
Jennings
Constitutive expression of a c ...
Apium graveolens Dulce Group
Plant J.
32
41-49
2002
-
1
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286404
Stoop
-
Characterization of NAD-depend ...
Apium graveolens
Plant Sci.
131
43-51
1998
-
-
-
-
-
-
8
6
-
-
-
-
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
1
-
-
-
-
8
-
6
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
286407
Jennings
Roles for mannitol and mannito ...
Nicotiana tabacum
Proc. Natl. Acad. Sci. USA
95
15129-15133
1998
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286405
Yamamoto
Subcellular localization of ce ...
Apium graveolens
Plant Physiol.
115
1397-1403
1997
-
-
-
-
-
-
-
-
4
-
1
-
-
2
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286406
Prata
Sugar repression of mannitol d ...
Apium graveolens
Plant Physiol.
114
307-314
1997
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286399
Stoop
-
Substrate stereospecificity of ...
Apium graveolens
Phytochemistry
43
1145-1150
1996
-
-
-
-
-
-
2
-
-
-
1
9
-
1
-
-
-
-
-
2
1
1
11
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
1
9
-
-
-
-
-
2
1
1
11
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286400
Stoop
Purification of NAD-dependent ...
Apium graveolens
Plant Physiol.
108
1219-1225
1995
-
-
-
-
-
-
-
-
-
-
1
1
-
5
-
-
1
-
-
2
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
2
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286401
Williamson
Sequence analysis of a mannito ...
Apium graveolens
Proc. Natl. Acad. Sci. USA
92
7148-7152
1995
-
-
-
-
-
-
-
-
-
-
-
1
-
8
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286402
Stoop
Effect of different carbon sou ...
Apium graveolens
Plant Physiol.
103
1001-1008
1993
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286403
Stoop
Partial purification and chara ...
Apium graveolens
Arch. Biochem. Biophys.
298
612-619
1992
-
-
-
-
-
-
2
2
-
-
-
1
-
3
-
-
1
-
-
3
1
1
3
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
2
-
-
-
1
-
-
-
1
-
3
1
1
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-