BRENDA - Enzyme Database show
show all sequences of 1.1.1.255

Substrate stereospecificity of the NAD-dependent mannitol dehydrogenase from celery

Stoop, J.M.H.; Chilton, W.S.; Pharr, D.M.; Phytochemistry 43, 1145-1150 (1996)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
D-mannose
competitive inhibition, Ki: 200 mM
Apium graveolens
D-threose
competitive inhibition, Ki: 18 mM
Apium graveolens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
1 * 40000, SDS-PAGE
Apium graveolens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-arabinitol + NAD+
Apium graveolens
-
D-lyxose + NADH + H+
-
Apium graveolens
r
D-iditol + NAD+
Apium graveolens
-
D-idose + NADH
-
Apium graveolens
r
D-mannitol + NAD+
Apium graveolens
highest activity, aldoses having 2S chirality are not reducible, with the exception of D-idose, marked preference for aldopentose and aldohexose, same stereospecificity in either direction with the same absolute configuration at C-2
D-mannose + NADH + H+
-
Apium graveolens
r
D-talose + NADH
Apium graveolens
-
D-altritol + NAD+
-
Apium graveolens
ir
D-threitol + NAD+
Apium graveolens
25% activity
D-threose + NADH
-
Apium graveolens
r
i-erythreitol + NAD+
Apium graveolens
16% activity
? + NADH
-
Apium graveolens
?
i-ribitol + NAD+
Apium graveolens
-
L-ribose + NADH
-
Apium graveolens
r
L-arabinitol + NAD+
Apium graveolens
-
L-arabinose + NADH
-
Apium graveolens
r
L-iditol + NAD+
Apium graveolens
less than 10% activity
L-idose + NADH
-
Apium graveolens
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Apium graveolens
-
celery
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Apium graveolens
-
culture condition:mannitol-grown cell
-
Apium graveolens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.32
-
D-mannitol
Apium graveolens
Storage Stability
Storage Stability
Organism
-80°C, stable for several months
Apium graveolens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinitol + NAD+
-
286399
Apium graveolens
D-lyxose + NADH + H+
-
286399
Apium graveolens
r
D-glucitol + NAD+
not oxidized by higher plants
286399
Apium graveolens
D-gulose + NADH
-
286399
Apium graveolens
r
D-iditol + NAD+
-
286399
Apium graveolens
D-idose + NADH
-
286399
Apium graveolens
r
D-mannitol + NAD+
highest activity, aldoses having 2S chirality are not reducible, with the exception of D-idose, marked preference for aldopentose and aldohexose, same stereospecificity in either direction with the same absolute configuration at C-2
286399
Apium graveolens
D-mannose + NADH + H+
-
286399
Apium graveolens
r
D-talose + NADH
-
286399
Apium graveolens
D-altritol + NAD+
-
286399
Apium graveolens
ir
D-threitol + NAD+
25% activity
286399
Apium graveolens
D-threose + NADH
-
286399
Apium graveolens
r
i-erythreitol + NAD+
16% activity
286399
Apium graveolens
? + NADH
-
286399
Apium graveolens
?
i-galactitol + NAD+
not oxidized by higher plants
286399
Apium graveolens
L-galactose + NADH
-
286399
Apium graveolens
r
i-ribitol + NAD+
-
286399
Apium graveolens
L-ribose + NADH
-
286399
Apium graveolens
r
L-arabinitol + NAD+
-
286399
Apium graveolens
L-arabinose + NADH
-
286399
Apium graveolens
r
L-iditol + NAD+
less than 10% activity
286399
Apium graveolens
L-idose + NADH
-
286399
Apium graveolens
r
Subunits
Subunits
Commentary
Organism
monomer
1 * 40000, SDS-PAGE
Apium graveolens
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Apium graveolens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Apium graveolens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
D-mannose
competitive inhibition, Ki: 200 mM
Apium graveolens
D-threose
competitive inhibition, Ki: 18 mM
Apium graveolens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
1 * 40000, SDS-PAGE
Apium graveolens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-arabinitol + NAD+
Apium graveolens
-
D-lyxose + NADH + H+
-
Apium graveolens
r
D-iditol + NAD+
Apium graveolens
-
D-idose + NADH
-
Apium graveolens
r
D-mannitol + NAD+
Apium graveolens
highest activity, aldoses having 2S chirality are not reducible, with the exception of D-idose, marked preference for aldopentose and aldohexose, same stereospecificity in either direction with the same absolute configuration at C-2
D-mannose + NADH + H+
-
Apium graveolens
r
D-talose + NADH
Apium graveolens
-
D-altritol + NAD+
-
Apium graveolens
ir
D-threitol + NAD+
Apium graveolens
25% activity
D-threose + NADH
-
Apium graveolens
r
i-erythreitol + NAD+
Apium graveolens
16% activity
? + NADH
-
Apium graveolens
?
i-ribitol + NAD+
Apium graveolens
-
L-ribose + NADH
-
Apium graveolens
r
L-arabinitol + NAD+
Apium graveolens
-
L-arabinose + NADH
-
Apium graveolens
r
L-iditol + NAD+
Apium graveolens
less than 10% activity
L-idose + NADH
-
Apium graveolens
r
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Apium graveolens
-
culture condition:mannitol-grown cell
-
Apium graveolens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.32
-
D-mannitol
Apium graveolens
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, stable for several months
Apium graveolens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinitol + NAD+
-
286399
Apium graveolens
D-lyxose + NADH + H+
-
286399
Apium graveolens
r
D-glucitol + NAD+
not oxidized by higher plants
286399
Apium graveolens
D-gulose + NADH
-
286399
Apium graveolens
r
D-iditol + NAD+
-
286399
Apium graveolens
D-idose + NADH
-
286399
Apium graveolens
r
D-mannitol + NAD+
highest activity, aldoses having 2S chirality are not reducible, with the exception of D-idose, marked preference for aldopentose and aldohexose, same stereospecificity in either direction with the same absolute configuration at C-2
286399
Apium graveolens
D-mannose + NADH + H+
-
286399
Apium graveolens
r
D-talose + NADH
-
286399
Apium graveolens
D-altritol + NAD+
-
286399
Apium graveolens
ir
D-threitol + NAD+
25% activity
286399
Apium graveolens
D-threose + NADH
-
286399
Apium graveolens
r
i-erythreitol + NAD+
16% activity
286399
Apium graveolens
? + NADH
-
286399
Apium graveolens
?
i-galactitol + NAD+
not oxidized by higher plants
286399
Apium graveolens
L-galactose + NADH
-
286399
Apium graveolens
r
i-ribitol + NAD+
-
286399
Apium graveolens
L-ribose + NADH
-
286399
Apium graveolens
r
L-arabinitol + NAD+
-
286399
Apium graveolens
L-arabinose + NADH
-
286399
Apium graveolens
r
L-iditol + NAD+
less than 10% activity
286399
Apium graveolens
L-idose + NADH
-
286399
Apium graveolens
r
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 40000, SDS-PAGE
Apium graveolens
Other publictions for EC 1.1.1.255
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
700868
Cheng
Salicylic acid stimulates secr ...
Apium graveolens
Planta
230
1093-1103
2009
1
-
1
-
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1
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1
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4
-
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1
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1
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1
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1
1
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1
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1
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686483
Mustorp
Detection of celery (Apium gra ...
Apium graveolens
Eur. Food Res. Technol.
226
771-778
2008
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1
1
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1
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656997
Jennings
Constitutive expression of a c ...
Apium graveolens Dulce Group
Plant J.
32
41-49
2002
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1
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-
-
-
-
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9
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286404
Stoop
-
Characterization of NAD-depend ...
Apium graveolens
Plant Sci.
131
43-51
1998
-
-
-
-
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8
6
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-
-
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1
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-
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2
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2
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-
-
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-
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2
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1
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1
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8
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6
-
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-
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2
-
2
-
-
-
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-
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2
-
-
-
-
-
-
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-
286407
Jennings
Roles for mannitol and mannito ...
Nicotiana tabacum
Proc. Natl. Acad. Sci. USA
95
15129-15133
1998
-
-
-
-
-
-
-
-
-
-
-
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4
-
-
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3
-
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3
-
-
-
-
-
-
-
-
-
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-
-
-
-
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-
286405
Yamamoto
Subcellular localization of ce ...
Apium graveolens
Plant Physiol.
115
1397-1403
1997
-
-
-
-
-
-
-
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4
-
1
-
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2
-
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4
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4
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1
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4
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286406
Prata
Sugar repression of mannitol d ...
Apium graveolens
Plant Physiol.
114
307-314
1997
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-
-
-
-
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-
-
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2
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286399
Stoop
-
Substrate stereospecificity of ...
Apium graveolens
Phytochemistry
43
1145-1150
1996
-
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-
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2
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1
9
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1
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2
1
1
11
1
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286400
Stoop
Purification of NAD-dependent ...
Apium graveolens
Plant Physiol.
108
1219-1225
1995
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5
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286401
Williamson
Sequence analysis of a mannito ...
Apium graveolens
Proc. Natl. Acad. Sci. USA
92
7148-7152
1995
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286402
Stoop
Effect of different carbon sou ...
Apium graveolens
Plant Physiol.
103
1001-1008
1993
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286403
Stoop
Partial purification and chara ...
Apium graveolens
Arch. Biochem. Biophys.
298
612-619
1992
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