BRENDA - Enzyme Database show
show all sequences of 1.1.1.254

Purification and properties of a new enzyme, D-carnitine dehydrogenase from Agrobacterium sp. 525a

Setyahida, S.; Ueyama, T.; Arimoto, T.; Mori, N.; Kitamoto, Y.; Biosci. Biotechnol. Biochem. 61, 1055-1058 (1997)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoate)
D-carnitine dehydrogenase, 1 mM, complete inactivation
Agrobacterium sp. 525a
Ag+
D-carnitine dehydrogenase, 1 mM, complete inactivation
Agrobacterium sp. 525a
Cu2+
D-carnitine dehydrogenase, 1 mM, complete inactivation
Agrobacterium sp. 525a
Hg2+
D-carnitine dehydrogenase, 1 mM, complete inactivation
Agrobacterium sp. 525a
N-ethylmaleimide
D-carnitine dehydrogenase, 1 mM, 77% inactivation after 3 min
Agrobacterium sp. 525a
p-chloromercuribenzoate
D-carnitine dehydrogenase, 0.1 mM, complete inactivation
Agrobacterium sp. 525a
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.06
-
NADH
D-carnitine dehydrogenase
Agrobacterium sp. 525a
0.07
-
NAD+
D-carnitine dehydrogenase
Agrobacterium sp. 525a
0.8
-
3-dehydrocarnitine
D-carnitine dehydrogenase
Agrobacterium sp. 525a
3.1
-
D-carnitine
D-carnitine dehydrogenase
Agrobacterium sp. 525a
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
D-carnitine dehydrogenase, 0.5 mM, 17% increase in activity, 1.0 mM 30% increase in activity, 5.0 mM, 35% increase in activity
Agrobacterium sp. 525a
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
3 * 29000, D-carnitine dehydrogenase, SDS-PAGE
Agrobacterium sp. 525a
86000
-
gel filtration, D-carnitine dehydrogenase
Agrobacterium sp. 525a
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-3-hydroxy-4-trimethylaminobutyrate + NAD+
Agrobacterium sp. 525a
-
3-dehydrocarnitine + NADH + H+
-
Agrobacterium sp. 525a
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Agrobacterium sp. 525a
-
-
-
Purification (Commentary)
Commentary
Organism
DEAE-Toyopearl, ammonium sulfate, Sephadex G-75, 5'-AMP-Sepharose 4B, Mono Q, TSK-gel G3000 SW, D-carnitine dehydrogenase
Agrobacterium sp. 525a
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
30.7
-
D-carnitine dehydrogenase
Agrobacterium sp. 525a
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-3-hydroxy-4-trimethylaminobutyrate + NAD+
-
285735
Agrobacterium sp. 525a
3-dehydrocarnitine + NADH + H+
-
285735
Agrobacterium sp. 525a
r
(S)-3-hydroxy-4-trimethylaminobutyrate + NAD+
D-carnitine dehydrogenase
285735
Agrobacterium sp. 525a
3-dehydrocarnitine + NADH + H+
-
285735
Agrobacterium sp. 525a
r
3-hydroxy-2-butanone + NAD+
enzyme specific for D-carnitine
285735
Agrobacterium sp. 525a
2,3-butandione + NADH
-
285735
Agrobacterium sp. 525a
?
DL-4-amino-3-hydroxy-n-butyrate + NAD+
D-carnitine dehydrogenase
285735
Agrobacterium sp. 525a
DL-4-amino-3-keto-n-butyrate + NADH
-
285735
Agrobacterium sp. 525a
?
Subunits
Subunits
Commentary
Organism
trimer
3 * 29000, D-carnitine dehydrogenase, SDS-PAGE
Agrobacterium sp. 525a
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
D-carnitine dehydrogenase
Agrobacterium sp. 525a
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
D-carnitine dehydrogenase, enzyme is rapidly inactivated above 40°C
Agrobacterium sp. 525a
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7
D-carnitine dehydrogenase, reduction of 3-dehydrocarnitine
Agrobacterium sp. 525a
9.3
-
D-carnitine dehydrogenase, oxidation of D-carnitine
Agrobacterium sp. 525a
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
9
D-carnitine dehydrogenase
Agrobacterium sp. 525a
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Agrobacterium sp. 525a
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Agrobacterium sp. 525a
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoate)
D-carnitine dehydrogenase, 1 mM, complete inactivation
Agrobacterium sp. 525a
Ag+
D-carnitine dehydrogenase, 1 mM, complete inactivation
Agrobacterium sp. 525a
Cu2+
D-carnitine dehydrogenase, 1 mM, complete inactivation
Agrobacterium sp. 525a
Hg2+
D-carnitine dehydrogenase, 1 mM, complete inactivation
Agrobacterium sp. 525a
N-ethylmaleimide
D-carnitine dehydrogenase, 1 mM, 77% inactivation after 3 min
Agrobacterium sp. 525a
p-chloromercuribenzoate
D-carnitine dehydrogenase, 0.1 mM, complete inactivation
Agrobacterium sp. 525a
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.06
-
NADH
D-carnitine dehydrogenase
Agrobacterium sp. 525a
0.07
-
NAD+
D-carnitine dehydrogenase
Agrobacterium sp. 525a
0.8
-
3-dehydrocarnitine
D-carnitine dehydrogenase
Agrobacterium sp. 525a
3.1
-
D-carnitine
D-carnitine dehydrogenase
Agrobacterium sp. 525a
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
D-carnitine dehydrogenase, 0.5 mM, 17% increase in activity, 1.0 mM 30% increase in activity, 5.0 mM, 35% increase in activity
Agrobacterium sp. 525a
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
3 * 29000, D-carnitine dehydrogenase, SDS-PAGE
Agrobacterium sp. 525a
86000
-
gel filtration, D-carnitine dehydrogenase
Agrobacterium sp. 525a
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-3-hydroxy-4-trimethylaminobutyrate + NAD+
Agrobacterium sp. 525a
-
3-dehydrocarnitine + NADH + H+
-
Agrobacterium sp. 525a
r
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE-Toyopearl, ammonium sulfate, Sephadex G-75, 5'-AMP-Sepharose 4B, Mono Q, TSK-gel G3000 SW, D-carnitine dehydrogenase
Agrobacterium sp. 525a
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
30.7
-
D-carnitine dehydrogenase
Agrobacterium sp. 525a
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-3-hydroxy-4-trimethylaminobutyrate + NAD+
-
285735
Agrobacterium sp. 525a
3-dehydrocarnitine + NADH + H+
-
285735
Agrobacterium sp. 525a
r
(S)-3-hydroxy-4-trimethylaminobutyrate + NAD+
D-carnitine dehydrogenase
285735
Agrobacterium sp. 525a
3-dehydrocarnitine + NADH + H+
-
285735
Agrobacterium sp. 525a
r
3-hydroxy-2-butanone + NAD+
enzyme specific for D-carnitine
285735
Agrobacterium sp. 525a
2,3-butandione + NADH
-
285735
Agrobacterium sp. 525a
?
DL-4-amino-3-hydroxy-n-butyrate + NAD+
D-carnitine dehydrogenase
285735
Agrobacterium sp. 525a
DL-4-amino-3-keto-n-butyrate + NADH
-
285735
Agrobacterium sp. 525a
?
Subunits (protein specific)
Subunits
Commentary
Organism
trimer
3 * 29000, D-carnitine dehydrogenase, SDS-PAGE
Agrobacterium sp. 525a
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
D-carnitine dehydrogenase
Agrobacterium sp. 525a
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
D-carnitine dehydrogenase, enzyme is rapidly inactivated above 40°C
Agrobacterium sp. 525a
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7
D-carnitine dehydrogenase, reduction of 3-dehydrocarnitine
Agrobacterium sp. 525a
9.3
-
D-carnitine dehydrogenase, oxidation of D-carnitine
Agrobacterium sp. 525a
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
9
D-carnitine dehydrogenase
Agrobacterium sp. 525a
Other publictions for EC 1.1.1.254
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
654037
Kluettermann
-
Synthesis of poly-beta-hydroxy ...
Agrobacterium tumefaciens
Acta Biotechnol.
22
261-269
2002
-
1
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285734
Obon
Enzymatic cycling assay for D- ...
Agrobacterium sp.
Anal. Biochem.
274
34-39
1999
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9587
Setyahadi
-
Production of L-carnitine from ...
Agrobacterium sp.
J. Mol. Catal. , B Enzym.
4
205-209
1998
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9588
Hanschmann
-
Conversion of D-carnitine into ...
Agrobacterium sp.
Biotechnol. Lett.
19
679-682
1997
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9589
Hanschmann
Purification and characterizat ...
Agrobacterium sp.
Biochim. Biophys. Acta
1337
133-142
1997
-
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17
5
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2
2
2
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4
-
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1
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1
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6
1
1
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1
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2
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1
1
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1
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17
-
5
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2
2
2
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1
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1
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6
1
1
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1
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2
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1
-
-
-
-
-
-
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285735
Setyahida
Purification and properties of ...
Agrobacterium sp. 525a
Biosci. Biotechnol. Biochem.
61
1055-1058
1997
-
-
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-
-
6
4
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1
2
1
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2
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1
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1
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4
1
1
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1
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2
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1
1
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1
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6
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4
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1
2
1
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4
1
1
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1
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2
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1
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