BRENDA - Enzyme Database show
show all sequences of 1.1.1.252

Deoxygenation of polyphenols by ascomycetes: kinetic behavior of the NADPH-dependent naphthol dehydrogenase and inhibition by tricyclazole and its analogues

Viviani, F.; Vors, J.P.; Gaudry, M.; Marquet, A.; Bull. Soc. Chim. Fr. 130, 395-404 (1993)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,3,8-Trihydroxynaphthalene
competitive to 1,3,6,8-tetrahydroxynaphthalene
Magnaporthe oryzae
Tricyclazole
noncompetitive with respect to 1,3,6,8-tetrahydroxynaphthalene, uncompetitive with respect to 1,3,8-trihydroxynaphthalene
Magnaporthe oryzae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0087
-
NADPH
-
Magnaporthe oryzae
0.079
-
1,3,6,8-Tetrahydroxynaphthalene
-
Magnaporthe oryzae
0.22
-
Scytalone
-
Magnaporthe oryzae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Magnaporthe oryzae
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,3,6,8-Tetrahydroxynaphthalene + NADPH
r
9575
Magnaporthe oryzae
Scytalone + NADP+
-
-
-
-
1,3,6,8-Tetrahydroxynaphthalene + NADPH
spefific for NADPH
9575
Magnaporthe oryzae
Scytalone + NADP+
-
-
-
-
1,3,8-trihydroxynaphthalene + NADPH + H+
specific for NADPH
9575
Magnaporthe oryzae
vermelone + NADP+
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
reduction of 1,3,6,8-tetrahydroxynaphthalene
Magnaporthe oryzae
7.5
-
oxidation of scytalone
Magnaporthe oryzae
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.2
6.5
pH 4.2: about 40% of maximal activity, pH 6.5: about 30% of maximal activity
Magnaporthe oryzae
7
8
pH 7.0: about 95% of maximal activity, pH 8.0: about 80% of maximal activity
Magnaporthe oryzae
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Magnaporthe oryzae
NADPH
-
Magnaporthe oryzae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Magnaporthe oryzae
NADPH
-
Magnaporthe oryzae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,3,8-Trihydroxynaphthalene
competitive to 1,3,6,8-tetrahydroxynaphthalene
Magnaporthe oryzae
Tricyclazole
noncompetitive with respect to 1,3,6,8-tetrahydroxynaphthalene, uncompetitive with respect to 1,3,8-trihydroxynaphthalene
Magnaporthe oryzae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0087
-
NADPH
-
Magnaporthe oryzae
0.079
-
1,3,6,8-Tetrahydroxynaphthalene
-
Magnaporthe oryzae
0.22
-
Scytalone
-
Magnaporthe oryzae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,3,6,8-Tetrahydroxynaphthalene + NADPH
r
9575
Magnaporthe oryzae
Scytalone + NADP+
-
-
-
-
1,3,6,8-Tetrahydroxynaphthalene + NADPH
spefific for NADPH
9575
Magnaporthe oryzae
Scytalone + NADP+
-
-
-
-
1,3,8-trihydroxynaphthalene + NADPH + H+
specific for NADPH
9575
Magnaporthe oryzae
vermelone + NADP+
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
reduction of 1,3,6,8-tetrahydroxynaphthalene
Magnaporthe oryzae
7.5
-
oxidation of scytalone
Magnaporthe oryzae
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.2
6.5
pH 4.2: about 40% of maximal activity, pH 6.5: about 30% of maximal activity
Magnaporthe oryzae
7
8
pH 7.0: about 95% of maximal activity, pH 8.0: about 80% of maximal activity
Magnaporthe oryzae
Other publictions for EC 1.1.1.252
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723949
Schaetzle
Tetrahydroxynaphthalene reduct ...
Magnaporthe grisea
Angew. Chem. Int. Ed. Engl.
51
2643-2646
2012
-
-
-
-
1
-
-
-
-
-
-
1
-
2
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-
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-
14
-
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1
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1
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1
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1
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-
14
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
697274
Brunskole
Trihydroxynaphthalene reductas ...
Curvularia lunata
Chem. Biol. Interact.
178
259-267
2009
-
1
1
-
-
-
27
1
-
-
-
-
-
7
-
-
1
-
-
-
1
-
1
-
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-
-
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-
1
14
-
14
-
1
1
1
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-
-
14
27
14
1
-
-
-
-
-
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-
1
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-
1
-
1
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697275
Stojan
Simultaneous binding of coenzy ...
Curvularia lunata
Chem. Biol. Interact.
178
268-273
2009
-
-
1
-
-
-
2
-
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-
2
-
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1
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1
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1
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1
1
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2
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1
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1
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697279
Kristan
Two homologous fungal carbonyl ...
Curvularia lunata
Chem. Biol. Interact.
178
295-302
2009
-
-
1
-
-
-
11
-
-
-
-
1
-
4
-
-
1
-
-
-
-
-
12
-
1
-
-
-
1
-
-
-
-
-
10
-
-
1
-
-
-
-
10
11
-
-
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-
-
1
-
-
-
1
-
-
-
-
12
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
713194
Zhang
-
Resistance development in rice ...
Magnaporthe grisea
Pestic. Biochem. Physiol.
94
43-47
2009
-
1
-
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-
-
1
-
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-
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-
1
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685550
Brunskole
Towards the first inhibitors o ...
Curvularia lunata
Bioorg. Med. Chem.
16
5881-5889
2008
-
1
1
-
-
-
7
-
-
-
-
1
-
4
-
-
1
1
-
-
-
-
2
1
-
-
-
-
1
-
-
1
6
-
-
-
1
1
1
-
-
-
-
7
6
-
-
-
-
1
-
-
-
1
-
-
-
-
2
1
-
-
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-
1
-
-
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688618
Rostkowski
Analysis of conformer stabilit ...
Fungi
J. Phys. Chem. B
111
8314-8320
2007
-
-
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-
-
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-
2
-
1
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4
1
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1
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1
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2
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4
1
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-
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-
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-
656846
Tsuji
Evidence for involvement of tw ...
Colletotrichum lagenaria
Mycol. Res.
107
854-860
2003
-
-
1
-
-
-
-
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1
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1
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3
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1
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1
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3
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-
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-
-
-
-
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-
-
9577
Thompson
2,3-Dihydro-2,5-dihydroxy-4H-b ...
Magnaporthe grisea
Anal. Biochem.
256
1-6
1998
-
-
-
-
-
-
-
1
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1
-
1
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4
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1
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1
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1
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4
-
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1
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-
-
-
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-
9571
Thompson
Trihydroxynaphthalene reductas ...
Magnaporthe grisea
Biochemistry
36
1852-1860
1997
-
-
-
-
-
-
1
6
-
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1
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3
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6
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3
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3
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3
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1
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6
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1
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6
-
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3
-
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-
9578
Andersson
A flexible lid controls access ...
Magnaporthe grisea
FEBS Lett.
400
173-176
1997
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1
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3
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9572
Andersson
Crystallization and preliminar ...
Magnaporthe grisea
Proteins Struct. Funct. Genet.
24
525-527
1996
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1
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1
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2
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1
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2
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2
1
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1
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1
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9573
Perpetua
Cloning and characterization o ...
Colletotrichum lagenaria
Mol. Plant Microbe Interact.
9
323-329
1996
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1
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1
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7
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2
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1
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1
1
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1
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2
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9576
Andersson
Crystal structure of the terna ...
Magnaporthe grisea
Structure
4
1161-1170
1996
-
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1
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1
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1
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4
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3
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3
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9570
Vidal-Cros
Polyhydroxynaphthalene reducta ...
Magnaporthe grisea
Eur. J. Biochem.
219
985-992
1994
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1
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3
1
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4
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1
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1
1
3
1
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1
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1
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1
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1
1
3
1
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9574
Ichinose
Post-aromatic deoxygenation in ...
Phialocephala lagerbergii
Chem. Pharm. Bull.
41
2015-2021
1993
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9575
Viviani
-
Deoxygenation of polyphenols b ...
Magnaporthe oryzae
Bull. Soc. Chim. Fr.
130
395-404
1993
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2
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9569
Wheeler
-
The inhibition of melanin bios ...
Magnaporthe oryzae
Exp. Mycol.
12
151-160
1988
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1
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8
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