BRENDA - Enzyme Database show
show all sequences of 1.1.1.252

Two homologous fungal carbonyl reductases with different substrate specificities

Kristan, K.; Brunskole, M.; Stojan, J.; Rizner, T.L.; Chem. Biol. Interact. 178, 295-302 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed as a glutathione S-transferase (GST)-fusion protein in Escherichia coli BL21 cells
Curvularia lunata
Inhibitors
Inhibitors
Commentary
Organism
Structure
3,7-dihydroxyflavone
-
Curvularia lunata
3-hydroxyflavone
-
Curvularia lunata
5,7-dihydroxyflavone
potent inhibitor of 3HNR
Curvularia lunata
5-hydroxyflavone
-
Curvularia lunata
5-methoxyflavone
-
Curvularia lunata
5-methyl-(1,2,4)-triazolo[3,4-b]benzothiazole
tricyclazole, specific inhibitor of 3HNR
Curvularia lunata
apigenin
potent inhibitor of 3HNR
Curvularia lunata
Biochanin A
-
Curvularia lunata
kaempferol
potent inhibitor of 3HNR
Curvularia lunata
luteolin
potent inhibitor of 3HNR
Curvularia lunata
naringenin
-
Curvularia lunata
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1,3,8-trihydroxynaphthalene + NADPH + H+
Curvularia lunata
-
vermelone + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Curvularia lunata
-
-
-
Purification (Commentary)
Commentary
Organism
by affinity binding followed by cleavage with thrombin
Curvularia lunata
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,3,8-trihydroxynaphthalene + NADPH + H+
-
697279
Curvularia lunata
vermelone + NADP+
-
-
-
?
1,4-androstadiene-3,17-dione + NADPH + H+
poor substrate of 3HNR
697279
Curvularia lunata
? + NADP+
-
-
-
?
2,(5alpha)-androstene-17-one + NADPH + H+
-
697279
Curvularia lunata
? + NADP+
-
-
-
?
2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one + NADP+
-
697279
Curvularia lunata
4,5-dihydroxy-2H-benzopyran-2-one + NADPH + H+
-
-
-
?
4-androstene-3,17-dione + NADPH + H+
poor substrate of 3HNR
697279
Curvularia lunata
? + NADP+
-
-
-
?
4-estrene-3,17-dione + NADPH + H+
poor substrate of 3HNR
697279
Curvularia lunata
? + NADP+
-
-
-
?
5-androstene-3beta-ol-17-one + NADPH + H+
-
697279
Curvularia lunata
? + NADP+
-
-
-
?
5alpha-androstane-3,17-dione + NADPH + H+
best steroid substrate for 3HNR
697279
Curvularia lunata
? + NADP+
-
-
-
?
5alpha-androstane-3-one + NADPH + H+
-
697279
Curvularia lunata
? + NADP+
-
-
-
?
5alpha-androstane-3alpha-ol-17-one + NADPH + H+
-
697279
Curvularia lunata
? + NADP+
-
-
-
?
5alpha-androstane-3beta-ol-17-one + NADPH + H+
-
697279
Curvularia lunata
? + NADP+
-
-
-
?
additional information
no activity with estrone
697279
Curvularia lunata
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
30
optimal temperature for oxidation of 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
Curvularia lunata
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
optimal pH for oxidation of 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
Curvularia lunata
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.0031
-
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
Curvularia lunata
apigenin
0.0058
-
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
Curvularia lunata
5,7-dihydroxyflavone
0.0068
-
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
Curvularia lunata
luteolin
0.0079
-
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
Curvularia lunata
kaempferol
0.012
-
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
Curvularia lunata
Biochanin A
0.025
-
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
Curvularia lunata
3,7-dihydroxyflavone
0.025
-
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
Curvularia lunata
3-hydroxyflavone
0.025
-
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
Curvularia lunata
5-hydroxyflavone
0.025
-
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
Curvularia lunata
5-methoxyflavone
0.026
-
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
Curvularia lunata
naringenin
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed as a glutathione S-transferase (GST)-fusion protein in Escherichia coli BL21 cells
Curvularia lunata
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.0031
-
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
Curvularia lunata
apigenin
0.0058
-
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
Curvularia lunata
5,7-dihydroxyflavone
0.0068
-
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
Curvularia lunata
luteolin
0.0079
-
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
Curvularia lunata
kaempferol
0.012
-
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
Curvularia lunata
Biochanin A
0.025
-
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
Curvularia lunata
3,7-dihydroxyflavone
0.025
-
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
Curvularia lunata
3-hydroxyflavone
0.025
-
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
Curvularia lunata
5-hydroxyflavone
0.025
-
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
Curvularia lunata
5-methoxyflavone
0.026
-
at 0.04 mM 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
Curvularia lunata
naringenin
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3,7-dihydroxyflavone
-
Curvularia lunata
3-hydroxyflavone
-
Curvularia lunata
5,7-dihydroxyflavone
potent inhibitor of 3HNR
Curvularia lunata
5-hydroxyflavone
-
Curvularia lunata
5-methoxyflavone
-
Curvularia lunata
5-methyl-(1,2,4)-triazolo[3,4-b]benzothiazole
tricyclazole, specific inhibitor of 3HNR
Curvularia lunata
apigenin
potent inhibitor of 3HNR
Curvularia lunata
Biochanin A
-
Curvularia lunata
kaempferol
potent inhibitor of 3HNR
Curvularia lunata
luteolin
potent inhibitor of 3HNR
Curvularia lunata
naringenin
-
Curvularia lunata
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1,3,8-trihydroxynaphthalene + NADPH + H+
Curvularia lunata
-
vermelone + NADP+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
by affinity binding followed by cleavage with thrombin
Curvularia lunata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,3,8-trihydroxynaphthalene + NADPH + H+
-
697279
Curvularia lunata
vermelone + NADP+
-
-
-
?
1,4-androstadiene-3,17-dione + NADPH + H+
poor substrate of 3HNR
697279
Curvularia lunata
? + NADP+
-
-
-
?
2,(5alpha)-androstene-17-one + NADPH + H+
-
697279
Curvularia lunata
? + NADP+
-
-
-
?
2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one + NADP+
-
697279
Curvularia lunata
4,5-dihydroxy-2H-benzopyran-2-one + NADPH + H+
-
-
-
?
4-androstene-3,17-dione + NADPH + H+
poor substrate of 3HNR
697279
Curvularia lunata
? + NADP+
-
-
-
?
4-estrene-3,17-dione + NADPH + H+
poor substrate of 3HNR
697279
Curvularia lunata
? + NADP+
-
-
-
?
5-androstene-3beta-ol-17-one + NADPH + H+
-
697279
Curvularia lunata
? + NADP+
-
-
-
?
5alpha-androstane-3,17-dione + NADPH + H+
best steroid substrate for 3HNR
697279
Curvularia lunata
? + NADP+
-
-
-
?
5alpha-androstane-3-one + NADPH + H+
-
697279
Curvularia lunata
? + NADP+
-
-
-
?
5alpha-androstane-3alpha-ol-17-one + NADPH + H+
-
697279
Curvularia lunata
? + NADP+
-
-
-
?
5alpha-androstane-3beta-ol-17-one + NADPH + H+
-
697279
Curvularia lunata
? + NADP+
-
-
-
?
additional information
no activity with estrone
697279
Curvularia lunata
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
30
optimal temperature for oxidation of 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
Curvularia lunata
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
optimal pH for oxidation of 2,3-dihydro-2,5-dihydroxy-4H-benzopyran-4-one
Curvularia lunata
Other publictions for EC 1.1.1.252
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723949
Schaetzle
Tetrahydroxynaphthalene reduct ...
Magnaporthe grisea
Angew. Chem. Int. Ed. Engl.
51
2643-2646
2012
-
-
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
697274
Brunskole
Trihydroxynaphthalene reductas ...
Curvularia lunata
Chem. Biol. Interact.
178
259-267
2009
-
1
1
-
-
-
27
1
-
-
-
-
-
7
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
14
-
14
-
1
1
1
-
-
-
14
27
14
1
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697275
Stojan
Simultaneous binding of coenzy ...
Curvularia lunata
Chem. Biol. Interact.
178
268-273
2009
-
-
1
-
-
-
2
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697279
Kristan
Two homologous fungal carbonyl ...
Curvularia lunata
Chem. Biol. Interact.
178
295-302
2009
-
-
1
-
-
-
11
-
-
-
-
1
-
4
-
-
1
-
-
-
-
-
12
-
1
-
-
-
1
-
-
-
-
-
10
-
-
1
-
-
-
-
10
11
-
-
-
-
-
1
-
-
-
1
-
-
-
-
12
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
713194
Zhang
-
Resistance development in rice ...
Magnaporthe grisea
Pestic. Biochem. Physiol.
94
43-47
2009
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685550
Brunskole
Towards the first inhibitors o ...
Curvularia lunata
Bioorg. Med. Chem.
16
5881-5889
2008
-
1
1
-
-
-
7
-
-
-
-
1
-
4
-
-
1
1
-
-
-
-
2
1
-
-
-
-
1
-
-
1
6
-
-
-
1
1
1
-
-
-
-
7
6
-
-
-
-
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
688618
Rostkowski
Analysis of conformer stabilit ...
Fungi
J. Phys. Chem. B
111
8314-8320
2007
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
1
-
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-
1
-
-
-
-
-
-
-
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-
2
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656846
Tsuji
Evidence for involvement of tw ...
Colletotrichum lagenaria
Mycol. Res.
107
854-860
2003
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
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-
1
1
-
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9577
Thompson
2,3-Dihydro-2,5-dihydroxy-4H-b ...
Magnaporthe grisea
Anal. Biochem.
256
1-6
1998
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
1
-
-
-
-
-
-
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-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
9571
Thompson
Trihydroxynaphthalene reductas ...
Magnaporthe grisea
Biochemistry
36
1852-1860
1997
-
-
-
-
-
-
1
6
-
-
-
1
-
3
-
-
-
-
-
-
-
-
6
-
-
-
-
3
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
1
-
6
-
-
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
9578
Andersson
A flexible lid controls access ...
Magnaporthe grisea
FEBS Lett.
400
173-176
1997
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
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1
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-
-
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-
-
-
-
-
-
-
-
-
-
-
-
9572
Andersson
Crystallization and preliminar ...
Magnaporthe grisea
Proteins Struct. Funct. Genet.
24
525-527
1996
-
-
-
1
-
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1
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2
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1
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2
-
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2
1
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1
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-
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-
-
1
-
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-
-
-
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-
-
-
-
-
-
-
-
9573
Perpetua
Cloning and characterization o ...
Colletotrichum lagenaria
Mol. Plant Microbe Interact.
9
323-329
1996
-
-
1
-
-
-
-
-
-
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-
1
-
7
-
-
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-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
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-
1
1
-
-
-
-
-
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