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show all sequences of 1.1.1.250

Identification, purification, and characterization of a D-arabinitol-specific dehydrogenase from Candida tropicalis

Quong, M.W.; Miyada, C.G.; Switchenko, A.C.; Goodman, T.C.; Biochem. Biophys. Res. Commun. 196, 1323-1329 (1993)

Data extracted from this reference:

Application
Application
Commentary
Organism
analysis
the specificity of the enzyme makes it useful for the development of a simple and specific method for the measurement of D-arabinitol, a metabolite of the pathogenic Candida spp. which has been described as a marker for disseminated candidiasis
Candida tropicalis
General Stability
General Stability
Organism
Mg2+ enhances the stability
Candida tropicalis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
NAD+
-
Candida tropicalis
39.8
-
D-arabinitol
-
Candida tropicalis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
1-5 mM, 50% increase of activity
Candida tropicalis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
x * 31000, SDS-PAGE
Candida tropicalis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Candida tropicalis
-
-
-
Purification (Commentary)
Commentary
Organism
-
Candida tropicalis
Reaction
Reaction
Commentary
Organism
D-arabinitol + NAD+ = D-ribulose + NADH + H+
in the reverse reaction the enzyme catalyzes transfer of 4(S) hydrogen of NADH
Candida tropicalis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
96
-
-
Candida tropicalis
Storage Stability
Storage Stability
Organism
-80░C, stable
Candida tropicalis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinitol + NAD+
r
9566
Candida tropicalis
D-ribulose + NADH + H+
-
9566
Candida tropicalis
-
D-xylulose + NADH
1% of the activity with D-ribulose
9566
Candida tropicalis
?
-
-
-
-
Galactitol + NAD+
1.6% of the activity with D-arabinitol
9566
Candida tropicalis
?
-
-
-
-
L-Xylulose + NADH
3% of the activity with D-ribulose
9566
Candida tropicalis
?
-
-
-
-
Xylitol + NAD+
5% of the activity with D-arabinitol
9566
Candida tropicalis
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 31000, SDS-PAGE
Candida tropicalis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
11
oxidation of D-arabinitol
Candida tropicalis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
8
11
pH 8.0: about 40% of maximal activity, pH 8.5: about 60% of maximal activity, pH 10-11: maximal activity
Candida tropicalis
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
11
-
unstable above
Candida tropicalis
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Candida tropicalis
NADH
-
Candida tropicalis
Application (protein specific)
Application
Commentary
Organism
analysis
the specificity of the enzyme makes it useful for the development of a simple and specific method for the measurement of D-arabinitol, a metabolite of the pathogenic Candida spp. which has been described as a marker for disseminated candidiasis
Candida tropicalis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Candida tropicalis
NADH
-
Candida tropicalis
General Stability (protein specific)
General Stability
Organism
Mg2+ enhances the stability
Candida tropicalis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
NAD+
-
Candida tropicalis
39.8
-
D-arabinitol
-
Candida tropicalis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
1-5 mM, 50% increase of activity
Candida tropicalis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31000
-
x * 31000, SDS-PAGE
Candida tropicalis
Purification (Commentary) (protein specific)
Commentary
Organism
-
Candida tropicalis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
96
-
-
Candida tropicalis
Storage Stability (protein specific)
Storage Stability
Organism
-80░C, stable
Candida tropicalis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinitol + NAD+
r
9566
Candida tropicalis
D-ribulose + NADH + H+
-
9566
Candida tropicalis
-
D-xylulose + NADH
1% of the activity with D-ribulose
9566
Candida tropicalis
?
-
-
-
-
Galactitol + NAD+
1.6% of the activity with D-arabinitol
9566
Candida tropicalis
?
-
-
-
-
L-Xylulose + NADH
3% of the activity with D-ribulose
9566
Candida tropicalis
?
-
-
-
-
Xylitol + NAD+
5% of the activity with D-arabinitol
9566
Candida tropicalis
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 31000, SDS-PAGE
Candida tropicalis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
11
oxidation of D-arabinitol
Candida tropicalis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
8
11
pH 8.0: about 40% of maximal activity, pH 8.5: about 60% of maximal activity, pH 10-11: maximal activity
Candida tropicalis
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
11
-
unstable above
Candida tropicalis
Other publictions for EC 1.1.1.250
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721370
Kallnik
Properties of recombinant Stre ...
Thermotoga maritima
Appl. Microbiol. Biotechnol.
90
1285-1293
2011
-
-
1
-
-
-
-
10
-
3
2
-
-
1
-
-
-
-
-
1
-
-
3
1
1
1
2
6
2
1
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
10
-
3
2
-
-
-
-
-
-
1
-
-
3
1
1
1
2
6
2
1
-
-
-
-
-
-
6
6
9565
Wong
D-Arabitol metabolism in Candi ...
Candida albicans
J. Bacteriol.
175
6314-6320
1993
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9566
Quong
Identification, purification, ...
Candida tropicalis
Biochem. Biophys. Res. Commun.
196
1323-1329
1993
-
1
-
-
-
1
-
2
-
1
1
-
-
1
-
-
1
1
-
-
1
1
5
1
-
-
-
-
1
1
1
2
-
-
-
-
1
-
2
-
-
1
-
-
-
2
-
1
1
-
-
-
-
1
-
-
1
1
5
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-