BRENDA - Enzyme Database show
show all sequences of 1.1.1.250

Properties of recombinant Strep-tagged and untagged hyperthermophilic D-arabitol dehydrogenase from Thermotoga maritima

Kallnik, V.; Schulz, C.; Schultz, C.; Schweiger, P.; Deppenmeier, U.; Appl. Microbiol. Biotechnol. 90, 1285-1293 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene tm0297, located in an operon with tm0298 encoding a mannitol dehydrogenase, phylogenetic analysis, expression of the Strep-tagged enzyme in Escherichia coli
Thermotoga maritima
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.027
-
NADH
with D-ribulose, pH 7.5, 85C, recombinant Strep-tagged enzyme
Thermotoga maritima
0.031
-
NADH
with D-ribulose, pH 7.5, 85C, recombinant detagged enzyme
Thermotoga maritima
0.059
-
NAD+
with D-arabinitol, pH 8.5, 85C, recombinant detagged enzyme
Thermotoga maritima
0.069
-
NAD+
with D-arabinitol, pH 8.5, 85C, recombinant Strep-tagged enzyme
Thermotoga maritima
2.6
-
D-arabinitol
pH 8.5, 85C, recombinant detagged enzyme
Thermotoga maritima
7.1
-
D-arabinitol
pH 8.5, 85C, recombinant Strep-tagged enzyme
Thermotoga maritima
7.2
-
D-ribulose
pH 7.5, 85C, recombinant detagged enzyme
Thermotoga maritima
13.9
-
D-ribulose
pH 7.5, 85C, recombinant Strep-tagged enzyme
Thermotoga maritima
20
-
D-xylitol
pH 8.5, 85C, recombinant Strep-tagged enzyme
Thermotoga maritima
21.7
-
D-xylitol
pH 8.5, 85C, recombinant detagged enzyme
Thermotoga maritima
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
K+
twofold activation at 60 mM, contributes to the overall stability of the protein and ise not required for catalytic activity
Thermotoga maritima
Mg2+
twofold activation at 55 mM, contributes to the overall stability of the protein and is not required for catalytic activity
Thermotoga maritima
additional information
no effect on the activity by CaCl2 or NaCl
Thermotoga maritima
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27600
-
6 * 27600, SDS-PAGE
Thermotoga maritima
164000
-
native PAGE
Thermotoga maritima
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermotoga maritima
-
gene tm0297
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
Thermotoga maritima cannot use D-arabitol as a sole carbon source. Optimal growth temperature of Thermotoga maritima is 80C
Thermotoga maritima
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinitol + NAD+
-
721370
Thermotoga maritima
D-ribulose + NADH + H+
-
-
-
r
D-xylitol + NAD+
-
721370
Thermotoga maritima
D-xylulose + NADH + H+
-
-
-
r
additional information
the enzyme exclusively catalyzed the NAD(H)-dependent oxidoreduction of D-arabitol, D-xylitol, D-ribulose, or D-xylulose
721370
Thermotoga maritima
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homohexamer
6 * 27600, SDS-PAGE
Thermotoga maritima
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
85
-
-
Thermotoga maritima
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
50
85
inactive below 50C, optimal activity at 85C
Thermotoga maritima
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
additional information
-
the addition of the Strep-tag led to a destabilization of tmArDH resulting in the loss of thermostability
Thermotoga maritima
85
-
rapid inactivation of the recombinant Strep-tagged enzyme, while the detagged enzyme retains over 90% activity after 90 min at 85C
Thermotoga maritima
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
11
-
D-arabinitol
pH 8.5, 85C, recombinant Strep-tagged enzyme
Thermotoga maritima
11.2
-
D-xylitol
pH 8.5, 85C, recombinant Strep-tagged enzyme
Thermotoga maritima
16.6
-
D-xylitol
pH 8.5, 85C, recombinant detagged enzyme
Thermotoga maritima
18.7
-
D-arabinitol
pH 8.5, 85C, recombinant detagged enzyme
Thermotoga maritima
165.6
-
D-ribulose
pH 7.5, 85C, recombinant Strep-tagged enzyme
Thermotoga maritima
193.2
-
D-ribulose
pH 7.5, 85C, recombinant detagged enzyme
Thermotoga maritima
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
reduction reaction
Thermotoga maritima
8.5
-
oxidation reaction
Thermotoga maritima
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.5
9.5
40% and 10% of maximal activity at pH 5.5 and pH 8.5, no activity below pH 4.5 and above pH 9.5
Thermotoga maritima
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
dependent on
Thermotoga maritima
NADH
dependent on
Thermotoga maritima
Cloned(Commentary) (protein specific)
Commentary
Organism
gene tm0297, located in an operon with tm0298 encoding a mannitol dehydrogenase, phylogenetic analysis, expression of the Strep-tagged enzyme in Escherichia coli
Thermotoga maritima
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
dependent on
Thermotoga maritima
NADH
dependent on
Thermotoga maritima
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.027
-
NADH
with D-ribulose, pH 7.5, 85C, recombinant Strep-tagged enzyme
Thermotoga maritima
0.031
-
NADH
with D-ribulose, pH 7.5, 85C, recombinant detagged enzyme
Thermotoga maritima
0.059
-
NAD+
with D-arabinitol, pH 8.5, 85C, recombinant detagged enzyme
Thermotoga maritima
0.069
-
NAD+
with D-arabinitol, pH 8.5, 85C, recombinant Strep-tagged enzyme
Thermotoga maritima
2.6
-
D-arabinitol
pH 8.5, 85C, recombinant detagged enzyme
Thermotoga maritima
7.1
-
D-arabinitol
pH 8.5, 85C, recombinant Strep-tagged enzyme
Thermotoga maritima
7.2
-
D-ribulose
pH 7.5, 85C, recombinant detagged enzyme
Thermotoga maritima
13.9
-
D-ribulose
pH 7.5, 85C, recombinant Strep-tagged enzyme
Thermotoga maritima
20
-
D-xylitol
pH 8.5, 85C, recombinant Strep-tagged enzyme
Thermotoga maritima
21.7
-
D-xylitol
pH 8.5, 85C, recombinant detagged enzyme
Thermotoga maritima
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
K+
twofold activation at 60 mM, contributes to the overall stability of the protein and ise not required for catalytic activity
Thermotoga maritima
Mg2+
twofold activation at 55 mM, contributes to the overall stability of the protein and is not required for catalytic activity
Thermotoga maritima
additional information
no effect on the activity by CaCl2 or NaCl
Thermotoga maritima
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27600
-
6 * 27600, SDS-PAGE
Thermotoga maritima
164000
-
native PAGE
Thermotoga maritima
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
Thermotoga maritima cannot use D-arabitol as a sole carbon source. Optimal growth temperature of Thermotoga maritima is 80C
Thermotoga maritima
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinitol + NAD+
-
721370
Thermotoga maritima
D-ribulose + NADH + H+
-
-
-
r
D-xylitol + NAD+
-
721370
Thermotoga maritima
D-xylulose + NADH + H+
-
-
-
r
additional information
the enzyme exclusively catalyzed the NAD(H)-dependent oxidoreduction of D-arabitol, D-xylitol, D-ribulose, or D-xylulose
721370
Thermotoga maritima
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homohexamer
6 * 27600, SDS-PAGE
Thermotoga maritima
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
85
-
-
Thermotoga maritima
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
50
85
inactive below 50C, optimal activity at 85C
Thermotoga maritima
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
additional information
-
the addition of the Strep-tag led to a destabilization of tmArDH resulting in the loss of thermostability
Thermotoga maritima
85
-
rapid inactivation of the recombinant Strep-tagged enzyme, while the detagged enzyme retains over 90% activity after 90 min at 85C
Thermotoga maritima
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
11
-
D-arabinitol
pH 8.5, 85C, recombinant Strep-tagged enzyme
Thermotoga maritima
11.2
-
D-xylitol
pH 8.5, 85C, recombinant Strep-tagged enzyme
Thermotoga maritima
16.6
-
D-xylitol
pH 8.5, 85C, recombinant detagged enzyme
Thermotoga maritima
18.7
-
D-arabinitol
pH 8.5, 85C, recombinant detagged enzyme
Thermotoga maritima
165.6
-
D-ribulose
pH 7.5, 85C, recombinant Strep-tagged enzyme
Thermotoga maritima
193.2
-
D-ribulose
pH 7.5, 85C, recombinant detagged enzyme
Thermotoga maritima
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
reduction reaction
Thermotoga maritima
8.5
-
oxidation reaction
Thermotoga maritima
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.5
9.5
40% and 10% of maximal activity at pH 5.5 and pH 8.5, no activity below pH 4.5 and above pH 9.5
Thermotoga maritima
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.56
-
D-xylitol
pH 8.5, 85C, recombinant Strep-tagged enzyme
Thermotoga maritima
0.74
-
D-xylitol
pH 8.5, 85C, recombinant detagged enzyme
Thermotoga maritima
1.5
-
D-arabinitol
pH 8.5, 85C, recombinant Strep-tagged enzyme
Thermotoga maritima
7.2
-
D-arabinitol
pH 8.5, 85C, recombinant detagged enzyme
Thermotoga maritima
12
-
D-ribulose
pH 7.5, 85C, recombinant Strep-tagged enzyme
Thermotoga maritima
27
-
D-ribulose
pH 7.5, 85C, recombinant detagged enzyme
Thermotoga maritima
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.56
-
D-xylitol
pH 8.5, 85C, recombinant Strep-tagged enzyme
Thermotoga maritima
0.74
-
D-xylitol
pH 8.5, 85C, recombinant detagged enzyme
Thermotoga maritima
1.5
-
D-arabinitol
pH 8.5, 85C, recombinant Strep-tagged enzyme
Thermotoga maritima
7.2
-
D-arabinitol
pH 8.5, 85C, recombinant detagged enzyme
Thermotoga maritima
12
-
D-ribulose
pH 7.5, 85C, recombinant Strep-tagged enzyme
Thermotoga maritima
27
-
D-ribulose
pH 7.5, 85C, recombinant detagged enzyme
Thermotoga maritima
Other publictions for EC 1.1.1.250
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721370
Kallnik
Properties of recombinant Stre ...
Thermotoga maritima
Appl. Microbiol. Biotechnol.
90
1285-1293
2011
-
-
1
-
-
-
-
10
-
3
2
-
-
1
-
-
-
-
-
1
-
-
3
1
1
1
2
6
2
1
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
10
-
3
2
-
-
-
-
-
-
1
-
-
3
1
1
1
2
6
2
1
-
-
-
-
-
-
6
6
9565
Wong
D-Arabitol metabolism in Candi ...
Candida albicans
J. Bacteriol.
175
6314-6320
1993
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9566
Quong
Identification, purification, ...
Candida tropicalis
Biochem. Biophys. Res. Commun.
196
1323-1329
1993
-
1
-
-
-
1
-
2
-
1
1
-
-
1
-
-
1
1
-
-
1
1
5
1
-
-
-
-
1
1
1
2
-
-
-
-
1
-
2
-
-
1
-
-
-
2
-
1
1
-
-
-
-
1
-
-
1
1
5
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-