Cloned (Comment) | Organism |
---|---|
isozyme VvSDH1, sequence comparison and phylogenetic analysis and tree, recombinant expression | Vitis vinifera |
isozyme VvSDH2, sequence comparison and phylogenetic analysis and tree, recombinant expression | Vitis vinifera |
isozyme VvSDH3, sequence comparison and phylogenetic analysis and tree, recombinant expression | Vitis vinifera |
isozyme VvSDH4, sequence comparison and phylogenetic analysis and tree, recombinant expression | Vitis vinifera |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
NADP+ | pH 7.0, 30°C, recombinant isozyme VvSDH3 | Vitis vinifera | |
0.027 | - |
shikimate | pH 9.0, 30°C, recombinant isozyme VvSDH3 | Vitis vinifera | |
0.038 | - |
NADP+ | pH 9.0, 30°C, recombinant isozyme VvSDH3 | Vitis vinifera | |
0.046 | - |
3-dehydroshikimate | pH 7.0, 30°C, recombinant isozyme VvSDH3 | Vitis vinifera | |
0.0538 | - |
NADP+ | pH 9.0, 30°C, recombinant isozyme VvSDH4 | Vitis vinifera | |
0.07 | - |
shikimate | pH 9.0, 30°C, recombinant isozyme VvSDH1 | Vitis vinifera | |
0.114 | - |
NADP+ | pH 9.0, 30°C, recombinant isozyme VvSDH1 | Vitis vinifera | |
0.1468 | - |
shikimate | pH 9.0, 30°C, recombinant isozyme VvSDH4 | Vitis vinifera | |
0.155 | - |
shikimate | pH 7.0, 30°C, recombinant isozyme VvSDH1 | Vitis vinifera | |
0.157 | - |
3-dehydroshikimate | pH 7.0, 30°C, recombinant isozyme VvSDH1 | Vitis vinifera | |
0.217 | - |
NADP+ | pH 7.0, 30°C, recombinant isozyme VvSDH1 | Vitis vinifera | |
0.422 | - |
shikimate | pH 7.0, 30°C, recombinant isozyme VvSDH3 | Vitis vinifera | |
186 | - |
3-dehydroshikimate | pH 7.0, 30°C, recombinant isozyme VvSDH4 | Vitis vinifera |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | isozyme VvSDH3 contains a putative chloroplastic transit peptide | Vitis vinifera | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
shikimate + NADP+ | Vitis vinifera | - |
3-dehydroshikimate + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vitis vinifera | A0A168S2B8 | - |
- |
Vitis vinifera | A0A168S2G5 | - |
- |
Vitis vinifera | A0A168S2H6 | - |
- |
Vitis vinifera | D7TUX1 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
berry | - |
Vitis vinifera | - |
fruit | VvSDH isozymes expression is analysed in the main berry tissues (skin, pulp, and seeds) at three development stages (green stage, veraison, and maturity) to determine their spatial and temporal expression patterns, overview | Vitis vinifera | - |
pericarp | high expression is observed for isozyme VvSDH1 before veraison but its maximum expression level is reached after veraison | Vitis vinifera | - |
pericarp | the VvSDH3 expression level rises during the green stage. At veraison, its expression drops and stays very low throughout the ripening stage | Vitis vinifera | - |
pericarp | VvSDH2 expression is relatively stable during grape development, except for a peak of expression before veraison | Vitis vinifera | - |
pericarp | VvSDH4 expression is relatively stable during grape development, except for a peak of expression before veraison | Vitis vinifera | - |
seed | - |
Vitis vinifera | - |
seed | immature, highest expression level of VvSDH3 | Vitis vinifera | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | isozyme VvSDH1 also produces gallate from shikimate with NADP+ | Vitis vinifera | ? | - |
? | |
additional information | isozyme VvSDH2 does not produce gallate from shikimate | Vitis vinifera | ? | - |
? | |
additional information | isozyme VvSDH3 also produces gallate from shikimate with NADP+ with low activity | Vitis vinifera | ? | - |
? | |
additional information | isozyme VvSDH4 also produces gallate from shikimate with NADP+ with low activity | Vitis vinifera | ? | - |
? | |
shikimate + NADP+ | - |
Vitis vinifera | 3-dehydroshikimate + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
SDH | - |
Vitis vinifera |
VvSDH1 | - |
Vitis vinifera |
VvSDH2 | - |
Vitis vinifera |
VvSDH3 | - |
Vitis vinifera |
VvSDH4 | - |
Vitis vinifera |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Vitis vinifera |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.16 | - |
shikimate | pH 9.0, 30°C, recombinant isozyme VvSDH3 | Vitis vinifera | |
7.56 | - |
shikimate | pH 7.0, 30°C, recombinant isozyme VvSDH3 | Vitis vinifera | |
8.62 | - |
3-dehydroshikimate | pH 7.0, 30°C, recombinant isozyme VvSDH3 | Vitis vinifera | |
11.72 | - |
shikimate | pH 7.0, 30°C, recombinant isozyme VvSDH1 | Vitis vinifera | |
19.78 | - |
NADP+ | pH 7.0, 30°C, recombinant isozyme VvSDH3 | Vitis vinifera | |
26.11 | - |
NADP+ | pH 9.0, 30°C, recombinant isozyme VvSDH3 | Vitis vinifera | |
26.12 | - |
shikimate | pH 9.0, 30°C, recombinant isozyme VvSDH4 | Vitis vinifera | |
28.89 | - |
NADP+ | pH 9.0, 30°C, recombinant isozyme VvSDH4 | Vitis vinifera | |
30.7 | - |
NADP+ | pH 7.0, 30°C, recombinant isozyme VvSDH1 | Vitis vinifera | |
94.55 | - |
shikimate | pH 9.0, 30°C, recombinant isozyme VvSDH1 | Vitis vinifera | |
95.85 | - |
3-dehydroshikimate | pH 7.0, 30°C, recombinant isozyme VvSDH1 | Vitis vinifera | |
239 | - |
NADP+ | pH 9.0, 30°C, recombinant isozyme VvSDH1 | Vitis vinifera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
shikimate dehydrogenase assay at, 3-dehydroshikimate production | Vitis vinifera |
9 | - |
shikimate dehydrogenase assay at, gallate production | Vitis vinifera |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Vitis vinifera | |
NADPH | - |
Vitis vinifera |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetic analysis of VvSDH isozymes, isozyme VvSDH1 belongs to group I, and clusters with four characterized DQD/SDHs: AtSDH, Poptr1, JrSDH, and NtSDH1, group members share about 75% sequence identity | Vitis vinifera |
evolution | phylogenetic analysis of VvSDH isozymes, isozyme VvSDH2 belongs to group II, and clusters with Poptr2 and Poptr3, two characterized DQD/SDHs, and NtSDH2, group members share about 71% sequence identity | Vitis vinifera |
evolution | phylogenetic analysis of VvSDH isozymes, isozyme VvSDH3 belongs to group III, group members share about 85% sequence identity, four of them from different species (VvSDH3, CasSDH2, FvSDH1, and EgSDH3) accumulate gallic acid-based tannins | Vitis vinifera |
evolution | phylogenetic analysis of VvSDH isozymes, isozyme VvSDH4 belongs to group IV, and clusters with with Poptr5, a DQD/SDH characterized in Populus trichocarpa, group members share about 77% sequence identity | Vitis vinifera |
metabolism | in plants, the shikimate pathway provides aromatic amino acids that are used to generate numerous secondary metabolites, including phenolic compounds. In this pathway, shikimate dehydrogenases catalyse the reversible dehydrogenation of 3-dehydroshikimate to shikimate | Vitis vinifera |
metabolism | in plants, the shikimate pathway provides aromatic amino acids that are used to generate numerous secondary metabolites, including phenolic compounds. In this pathway, shikimate dehydrogenases catalyse the reversible dehydrogenation of 3-dehydroshikimate to shikimate. Gallic acid metabolism in grape berry tissues along development, overview | Vitis vinifera |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18 | - |
shikimate | pH 7.0, 30°C, recombinant isozyme VvSDH3 | Vitis vinifera | |
76 | - |
shikimate | pH 7.0, 30°C, recombinant isozyme VvSDH1 | Vitis vinifera | |
178 | - |
shikimate | pH 9.0, 30°C, recombinant isozyme VvSDH4 | Vitis vinifera | |
186 | - |
3-dehydroshikimate | pH 7.0, 30°C, recombinant isozyme VvSDH3 | Vitis vinifera | |
189 | - |
shikimate | pH 9.0, 30°C, recombinant isozyme VvSDH3 | Vitis vinifera | |
262 | - |
NADP+ | pH 7.0, 30°C, recombinant isozyme VvSDH1 | Vitis vinifera | |
537 | - |
NADP+ | pH 9.0, 30°C, recombinant isozyme VvSDH4 | Vitis vinifera | |
611 | - |
3-dehydroshikimate | pH 7.0, 30°C, recombinant isozyme VvSDH1 | Vitis vinifera | |
680 | - |
NADP+ | pH 9.0, 30°C, recombinant isozyme VvSDH3 | Vitis vinifera | |
862 | - |
NADP+ | pH 7.0, 30°C, recombinant isozyme VvSDH3 | Vitis vinifera | |
1051 | - |
shikimate | pH 9.0, 30°C, recombinant isozyme VvSDH1 | Vitis vinifera | |
1113 | - |
NADP+ | pH 9.0, 30°C, recombinant isozyme VvSDH1 | Vitis vinifera |