BRENDA - Enzyme Database show
show all sequences of 1.1.1.25

Functional analysis of the essential bifunctional tobacco enzyme 3-dehydroquinate dehydratase/shikimate dehydrogenase in transgenic tobacco plants

Ding, L.; Hofius, D.; Hajirezaei, M.R.; Fernie, A.R.; Boernke, F.; Sonnewald, U.; J. Exp. Bot. 58, 2053-2067 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
DNA and amino acid sequence determination and analysis; DNA and amino acid sequence determination and analysis, expression of Hs6-tagged isozyme 1 in Escherichia coli, transfection of Nicotina plants; expression in Escherichia coli; expression in Escherichia coli
Nicotiana tabacum
Engineering
Amino acid exchange
Commentary
Organism
additional information
cytosolic isoform cannot complement loss of the plastidial isoform; expression of endogenous DHD/SHD-1 is suppressed by RNAi in transgenic tobacco plants, the transgenic lines with less than 40% of wild-type activity display severe growth retardation and reduced content of aromatic amino acids and downstream products such as cholorogenic acid and lignin, but accumulation of dehydroquinate and shikimate, possibly due to existence of a parallel extra-plastidic shikimate pathway into which dehydroquinate is diverted with a second gene DHD/SHD-2 in tobacco lacking a plastidic targeting sequence, the cytosolic shikimate synthesis cannot complement loss of the plastidial pathway, phenotype, overview; expression of endogenous DHD/SHD-1 is suppressed by RNAi in transgenic tobacco plants, the transgenic lines with less than 40% of wild-type activity display severe growth retardation and reduced content of aromatic amino acids and downstream products such as cholorogenic acid and lignin, but accumulation of dehydroquinate and shikimate, possibly due to existence of a parallel extra-plastidic shikimate pathway into which dehydroquinate is diverted with a second gene DHD/SHD-2 in tobacco lacking a plastidic targeting sequence, the cytosolic shikimate synthesis cannot complement loss of the plastidial pathway, phenotype, overview; suppression of shikimate dehydrogenase activity by RNAi. Transgenic lines with less than 40% of wild-type activity display severe growth retardation and reduced content of aromatic amino acids and downstream products such as cholorogenic acid and lignin. Dehydroquinate, the substrate of the enzyme, accumulates, and due to a second, cytosolic enzyme, the product, shikimate accumulates
Nicotiana tabacum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.031
-
NADP+
22°C, pH 9.0; 22°C, pH 9.0
Nicotiana tabacum
0.13
-
shikimate
22°C, pH 9.0; 22°C, pH 9.0
Nicotiana tabacum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
DHD/SHD-1 containing a plastidic targeting sequence
Nicotiana tabacum
9507
-
cytosol
DHD/SHD-2; exclusively cytosolic
Nicotiana tabacum
5829
-
plastid
-
Nicotiana tabacum
9536
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-quinate + NADP+
Nicotiana tabacum
-
3-dehydroquinate + NADPH + H+
-
-
r
additional information
Nicotiana tabacum
the cytosolic shikimate synthesis cannot complement loss of the plastidial pathway
?
-
-
-
shikimate + NAD+
Nicotiana tabacum
-
3-dehydroshikimate + NADH + H+
-
-
r
shikimate + NADP+
Nicotiana tabacum
-
3-dehydroshikimate + NADPH + H+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Nicotiana tabacum
-
-
-
Nicotiana tabacum
Q6PUF9
DHD/SHD-1; plastidic isozyme
-
Nicotiana tabacum
Q6PUG0
cytosolic isoform; DHD/SHD-2; cytosolic isozyme
-
Purification (Commentary)
Commentary
Organism
recombinant protein; recombinant protein
Nicotiana tabacum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
;
Nicotiana tabacum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-dehydroshikimate + NADPH
-
681134
Nicotiana tabacum
shikimate + NADP+
-
-
-
?
L-quinate + NADP+
-
681134
Nicotiana tabacum
3-dehydroquinate + NADPH + H+
-
-
-
r
additional information
the cytosolic shikimate synthesis cannot complement loss of the plastidial pathway
681134
Nicotiana tabacum
?
-
-
-
-
additional information
the enzyme also shows 3-dehydroquinate dehydratase activity, EC 4.2.1.10
681134
Nicotiana tabacum
?
-
-
-
-
shikimate + NAD+
-
681134
Nicotiana tabacum
3-dehydroshikimate + NADH + H+
-
-
-
r
shikimate + NADP+
-
681134
Nicotiana tabacum
3-dehydroshikimate + NADPH + H+
-
-
-
?
shikimate + NADP+
-
681134
Nicotiana tabacum
3-dehydroshikimate + NADPH + H+
-
-
-
r
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at room temperature
Nicotiana tabacum
30
45
-
Nicotiana tabacum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
7.2
formation of shikimate
Nicotiana tabacum
9
9.4
formation of 3-dehydroshikimate
Nicotiana tabacum
9
-
assay at; assay at
Nicotiana tabacum
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
;
Nicotiana tabacum
NADPH
;
Nicotiana tabacum
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA and amino acid sequence determination and analysis, expression of Hs6-tagged isozyme 1 in Escherichia coli, transfection of Nicotina plants
Nicotiana tabacum
DNA and amino acid sequence determination and analysis; expression in Escherichia coli
Nicotiana tabacum
expression in Escherichia coli
Nicotiana tabacum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Nicotiana tabacum
NADPH
-
Nicotiana tabacum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
cytosolic isoform cannot complement loss of the plastidial isoform; expression of endogenous DHD/SHD-1 is suppressed by RNAi in transgenic tobacco plants, the transgenic lines with less than 40% of wild-type activity display severe growth retardation and reduced content of aromatic amino acids and downstream products such as cholorogenic acid and lignin, but accumulation of dehydroquinate and shikimate, possibly due to existence of a parallel extra-plastidic shikimate pathway into which dehydroquinate is diverted with a second gene DHD/SHD-2 in tobacco lacking a plastidic targeting sequence, the cytosolic shikimate synthesis cannot complement loss of the plastidial pathway, phenotype, overview
Nicotiana tabacum
additional information
expression of endogenous DHD/SHD-1 is suppressed by RNAi in transgenic tobacco plants, the transgenic lines with less than 40% of wild-type activity display severe growth retardation and reduced content of aromatic amino acids and downstream products such as cholorogenic acid and lignin, but accumulation of dehydroquinate and shikimate, possibly due to existence of a parallel extra-plastidic shikimate pathway into which dehydroquinate is diverted with a second gene DHD/SHD-2 in tobacco lacking a plastidic targeting sequence, the cytosolic shikimate synthesis cannot complement loss of the plastidial pathway, phenotype, overview
Nicotiana tabacum
additional information
suppression of shikimate dehydrogenase activity by RNAi. Transgenic lines with less than 40% of wild-type activity display severe growth retardation and reduced content of aromatic amino acids and downstream products such as cholorogenic acid and lignin. Dehydroquinate, the substrate of the enzyme, accumulates, and due to a second, cytosolic enzyme, the product, shikimate accumulates
Nicotiana tabacum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.031
-
NADP+
22°C, pH 9.0
Nicotiana tabacum
0.13
-
shikimate
22°C, pH 9.0
Nicotiana tabacum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
DHD/SHD-1 containing a plastidic targeting sequence
Nicotiana tabacum
9507
-
cytosol
DHD/SHD-2; exclusively cytosolic
Nicotiana tabacum
5829
-
plastid
-
Nicotiana tabacum
9536
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-quinate + NADP+
Nicotiana tabacum
-
3-dehydroquinate + NADPH + H+
-
-
r
additional information
Nicotiana tabacum
the cytosolic shikimate synthesis cannot complement loss of the plastidial pathway
?
-
-
-
shikimate + NAD+
Nicotiana tabacum
-
3-dehydroshikimate + NADH + H+
-
-
r
shikimate + NADP+
Nicotiana tabacum
-
3-dehydroshikimate + NADPH + H+
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant protein
Nicotiana tabacum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Nicotiana tabacum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-dehydroshikimate + NADPH
-
681134
Nicotiana tabacum
shikimate + NADP+
-
-
-
?
L-quinate + NADP+
-
681134
Nicotiana tabacum
3-dehydroquinate + NADPH + H+
-
-
-
r
additional information
the cytosolic shikimate synthesis cannot complement loss of the plastidial pathway
681134
Nicotiana tabacum
?
-
-
-
-
additional information
the enzyme also shows 3-dehydroquinate dehydratase activity, EC 4.2.1.10
681134
Nicotiana tabacum
?
-
-
-
-
shikimate + NAD+
-
681134
Nicotiana tabacum
3-dehydroshikimate + NADH + H+
-
-
-
r
shikimate + NADP+
-
681134
Nicotiana tabacum
3-dehydroshikimate + NADPH + H+
-
-
-
?
shikimate + NADP+
-
681134
Nicotiana tabacum
3-dehydroshikimate + NADPH + H+
-
-
-
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at room temperature
Nicotiana tabacum
30
45
-
Nicotiana tabacum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
7.2
formation of shikimate
Nicotiana tabacum
9
9.4
formation of 3-dehydroshikimate
Nicotiana tabacum
9
-
assay at
Nicotiana tabacum
Other publictions for EC 1.1.1.25
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724089
Kubota
Characterization of shikimate ...
Corynebacterium glutamicum, Corynebacterium glutamicum JCM 18229
Appl. Microbiol. Biotechnol.
97
8139-8149
2013
-
-
1
-
-
-
2
6
-
-
1
-
-
5
-
-
-
-
-
-
-
-
12
1
-
-
-
6
4
-
-
5
-
-
-
-
-
2
6
-
-
-
-
4
-
6
-
-
2
-
-
-
-
-
-
-
-
-
12
2
-
-
-
6
4
-
-
-
1
1
1
1
6
6
725977
Lee
High-resolution structure of s ...
Thermotoga maritima, Thermotoga maritima ATCC 43589
Mol. Cells
33
229-233
2012
-
-
-
1
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
721203
Lee
Overexpression, crystallizatio ...
Archaeoglobus fulgidus, Archaeoglobus fulgidus ATCC 49558
Acta Crystallogr. Sect. F
67
1556-1558
2011
-
1
1
1
-
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
723859
Lee
Overexpression, crystallizatio ...
Thermotoga maritima, Thermotoga maritima ATCC 43589
Acta Crystallogr. Sect. F
67
824-826
2011
-
-
1
1
-
-
-
-
-
-
2
-
-
5
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704148
Cao
Effect of BTH on anthocyanin c ...
Fragaria x ananassa
J. Agric. Food Chem.
58
5801-5805
2010
2
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
2
3
2
2
-
2
-
-
-
1
-
-
2
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
3
2
2
-
2
-
-
-
1
-
-
-
-
1
1
-
-
-
697894
Han
X-ray crystallographic and enz ...
Staphylococcus epidermidis RP62A
FEBS J.
276
1125-1139
2009
-
-
1
1
1
-
-
5
-
-
2
-
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
3
1
1
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
5
-
-
2
-
-
-
-
1
-
-
-
-
2
1
-
-
-
3
1
1
-
-
-
-
-
-
-
-
698320
Rodrigues
Homogeneous recombinant Mycoba ...
Mycobacterium tuberculosis
Int. J. Biol. Macromol.
45
200-205
2009
-
-
1
-
6
-
-
2
1
-
1
-
-
2
-
-
1
-
-
-
2
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
2
1
-
1
-
-
-
-
1
-
-
2
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
699631
Barcellos
Structural studies of shikimat ...
Bacillus anthracis
J. Mol. Model.
15
147-155
2009
-
-
-
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
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-
-
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-
-
1
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
711616
Rodrigues
The conserved Lysine69 residue ...
Mycobacterium tuberculosis, no activity in Homo sapiens
BMC Res. Notes
2
227
2009
-
1
-
-
1
-
-
5
-
-
-
1
-
4
-
-
-
1
-
-
-
-
2
1
1
-
-
2
1
-
-
2
-
-
-
-
1
-
2
-
1
-
-
-
-
5
-
-
-
1
-
-
-
-
-
-
-
-
2
1
1
-
-
2
1
-
-
-
-
2
2
-
4
4
684153
Schoepe
1.6 A structure of an NAD(+)-d ...
Corynebacterium glutamicum
Acta Crystallogr. Sect. D
64
803-809
2008
-
-
-
1
-
-
-
2
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
2
-
-
-
2
-
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-
-
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2
1
-
-
-
-
-
2
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-
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-
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-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
689956
Arcuri
Structural studies of shikimat ...
Mycobacterium tuberculosis
Proteins
72
720-730
2008
-
-
-
1
-
-
-
-
-
-
-
-
-
7
-
-
-
-
1
-
-
-
-
-
-
-
1
-
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-
-
-
-
-
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1
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-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
694093
Singh
A phylogenomic analysis of the ...
Pseudomonas putida, Pseudomonas putida KT2240, Pseudomonas putida KT2440
Mol. Biol. Evol.
25
2221-2232
2008
-
-
2
-
-
-
-
16
-
-
-
8
-
8
-
-
2
-
-
-
-
2
14
-
1
-
-
22
1
-
-
3
-
-
-
-
-
7
7
-
-
-
-
-
-
16
-
-
-
8
-
-
-
7
-
-
-
2
14
-
4
-
-
22
4
-
-
-
-
-
-
-
-
-
681134
Ding
Functional analysis of the ess ...
Nicotiana tabacum
J. Exp. Bot.
58
2053-2067
2007
-
-
1
-
1
-
-
2
3
-
-
6
-
3
-
-
1
-
-
1
-
-
11
-
2
-
-
-
3
-
-
2
-
-
-
-
-
3
4
-
3
-
-
-
-
4
3
-
-
6
-
-
-
2
-
2
-
-
11
-
4
-
-
-
4
-
-
-
-
-
-
-
-
-
684670
Fonseca
Kinetic and chemical mechanism ...
Mycobacterium tuberculosis
Arch. Biochem. Biophys.
457
123-133
2007
-
-
-
-
-
-
2
2
-
-
-
-
-
2
-
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
685190
Gan
Structural and biochemical ana ...
Aquifex aeolicus
Biochemistry
46
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2007
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686243
Singh
-
The DHQ-dehydroshikimate-SDH-s ...
Arabidopsis thaliana
Cryst. Growth Des.
7
2153-2160
2007
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1
1
8
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16
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1
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16
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Bagautdinov
Crystal structures of shikimat ...
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2007
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667097
Schoepe
Cloning, expression, purificat ...
Corynebacterium glutamicum
Acta Crystallogr. Sect. F
F62
635-637
2006
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1
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667700
Singh
Structure of Arabidopsis dehyd ...
Arabidopsis thaliana
Biochemistry
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2006
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1
1
10
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18
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10
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1
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3
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1
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18
1
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668019
Adachi
Purification and properties of ...
Gluconobacter oxydans, Gluconobacter oxydans IFO 3244
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2786-2789
2006
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2
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6
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1
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2
2
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2
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668576
Han
Biochemical characterization a ...
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3
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1
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1
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3
1
1
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670786
Fonseca
Functional shikimate dehydroge ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Protein Expr. Purif.
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429-437
2006
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Zhang
Expression, purification and p ...
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2
1
1
1
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1
2
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Singh
Crystal structure of a novel s ...
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2
1
1
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2
1
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655639
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A thermostable shikimate 5-deh ...
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238
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1
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2
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2
1
2
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655854
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The crystal structure of shiki ...
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2003
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2
1
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657359
Padyana
Crystal structure of shikimate ...
Methanocaldococcus jannaschii
Structure
11
1005-1013
2003
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1
1
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4
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1
2
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670901
Vogan
Shikimate dehydrogenase struct ...
Methanocaldococcus jannaschii
Structure
11
902-903
2003
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1
1
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657254
Magalhaes
Cloning and expression of func ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Protein Expr. Purif.
26
59-64
2002
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1
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1
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1
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286396
Maclean
Crystallization and preliminar ...
Escherichia coli
Acta Crystallogr. Sect. D
56
512-515
2000
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1
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1
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286398
Diaz
-
Shikimate dehydrogenase from p ...
Capsicum annuum
Physiol. Plant.
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147-152
1997
4
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2
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Lourenco
Purification and properties of ...
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1991
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5
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286386
Chaudhuri
The purification of shikimate ...
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Lemos Silva
Inhibition of shikimate dehydr ...
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Lourenco
-
Partial purification and some ...
Solanum lycopersicum
Phytochemistry
23
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2
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3
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8
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2
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1
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1
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1
1
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5466
Polley
Purification and characterizat ...
Physcomitrella patens
Biochim. Biophys. Acta
526
259-266
1978
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1
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1
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286388
Koshiba
Purification of two forms of t ...
Vigna mungo
Biochim. Biophys. Acta
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4
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1
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Jacobson
Purification and stability of ...
Neurospora crassa
Biochim. Biophys. Acta
289
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1972
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286390
Higuchi
-
Changes in activity of shikima ...
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1967
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1
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286389
Sanderson
5-Dehydroshikimate reductase i ...
Camellia sinensis
Biochem. J.
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Aromatic biosynthesis in highe ...
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Aromatic biosynthesis. XIV. 5- ...
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